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The map label for this gene is insB1 [H]

Identifier: 30063303

GI number: 30063303

Start: 1909479

End: 1909688

Strand: Reverse

Name: insB1 [H]

Synonym: S1973

Alternate gene names: 30063303

Gene position: 1909688-1909479 (Counterclockwise)

Preceding gene: 30063304

Following gene: 30063302

Centisome position: 41.52

GC content: 48.1

Gene sequence:

>210_bases
ATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCAT
TGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGTACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGC
TGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
GATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGG
CCTTTTAGGTCGTGGTATGG

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCAAAAAAAGCAACAGCGTATTTTGCACAGGAGTCGCTGAAAAATACGCGTTAATCGAACAATGGCG
ACAACAATTTCCCATTGAAG

Product: IS1 orfB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_69_residues
MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_68_residues
TDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=69, Percent_Identity=98.5507246376812, Blast_Score=139, Evalue=4e-35,
Organism=Escherichia coli, GI1788203, Length=69, Percent_Identity=98.5507246376812, Blast_Score=139, Evalue=4e-35,
Organism=Escherichia coli, GI1786203, Length=69, Percent_Identity=98.5507246376812, Blast_Score=139, Evalue=4e-35,
Organism=Escherichia coli, GI1787223, Length=69, Percent_Identity=97.1014492753623, Blast_Score=137, Evalue=1e-34,
Organism=Escherichia coli, GI1786470, Length=69, Percent_Identity=97.1014492753623, Blast_Score=136, Evalue=2e-34,
Organism=Escherichia coli, GI1786459, Length=69, Percent_Identity=97.1014492753623, Blast_Score=136, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 8338; Mature: 8207

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIG
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
HYLNIKHYQ
HHHCCCCCC
>Mature Secondary Structure 
TDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIG
CCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
HYLNIKHYQ
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]