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The map label for this gene is malI

Identifier: 30063135

GI number: 30063135

Start: 1714506

End: 1715534

Strand: Reverse

Name: malI

Synonym: S1777

Alternate gene names: 30063135

Gene position: 1715534-1714506 (Counterclockwise)

Preceding gene: 161486482

Following gene: 30063134

Centisome position: 37.3

GC content: 54.42

Gene sequence:

>1029_bases
ATGGCTACCGCCAAAAAAATAACCATTCATGATGTTGCGCTGGCTGCGGGCGTGTCGGTAAGTACCGTTTCGCTGGTGCT
TAATGGCAAAGGGCGAATCTCTACCGCCACAGGAGAACGCGTTAACGCCGCCATTGAAGAGCTGGGATTTGTGCGCAATC
GCCAGGCGTCGGCGCTGCGCGGCGGGCAAAGCGGCGTCATTGGTTTGATCGTCCGTGATTTATCTGCGCCGTTTTACGCC
GAATTGACGGCCGGATTGACGGAAGCTCTGGAAGCACAGGGACGGATGGTTTTTTTGCTTCACGGCGGTAAAGACGGCGA
GCAGCTGGCACAGCGGTTTTCACTGTTACTGAATCAGGGTGTCGATGGTGTGGTAATTGCCGGGGCTGCAGGAAGCAGCG
ATGACCTGCGGCGGATGGCAGAAGAAAAAGCTATCCCGGTGATTTTCGCTTCCCGTGCCAGTTATCTTGATGATGTTGAT
ACGGTTCGCCCGGACAACATGCAGGCTGCACAGTTGTTGACGGAGCATCTCATTCGCAATGGGCATCAGCGGATCGCCTG
GCTGGGAGGGCAAAGTTCCTCATTAACCCGTGCAGAACGGGTGGGGGGCTATTGTGCAACTCTACTAAAATTTGGCCTGC
CGTTTCACAGCGATTGGGTGTTGGAGTGCACTTCCAGCCAGAAGCAAGCCGCGGAAGCTATCACGGCGCTTTTACGTCAT
AACCCGACCATCAGTGCCGTGGTTTGCTATAACGAAACTATTGCGATGGGGGCATGGTTTGGTTTGCTGAAAGCAGGGCG
GCAAAGCGGGGAAAGCGGAGTCGATCGTTACTTTGAGCAACAGGTTTCGCTGGCGGCATTTACCGATGCGACACCAACCA
CACTTGATGATATACCTGTTACCTGGGCCAGCACGCCAGCGCGGGAACTTGGTATCACACTTGCGGATCGCATGATGCAA
AAAATCACCCATGAAGAGACGCATTCACGCAATCTTATTATTCCCGCCCGGCTCATTGCGGCGAAATAA

Upstream 100 bases:

>100_bases
GTGAGTCATAAATCACATTACGCAACGATAATAGCGGGTATAAGATAAATAAAAGGTAAAACGTTTTATCTGTCACATAA
TCAGGGAGTAGGTCATCTGC

Downstream 100 bases:

>100_bases
TCCTCTCTTTATCTGCTATACTTGGTAGTGTCCTTTCCTCAAGGTTAATGATAAAAAATGTGGCACACAGGCGGTGCCTT
TTATTTTTACTGAAGTTCAC

Product: DNA-binding transcriptional repressor MalI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYA
ELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQ
KITHEETHSRNLIIPARLIAAK

Sequences:

>Translated_342_residues
MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYA
ELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQ
KITHEETHSRNLIIPARLIAAK
>Mature_341_residues
ATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAE
LTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDT
VRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRHN
PTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQK
ITHEETHSRNLIIPARLIAAK

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=342, Percent_Identity=99.7076023391813, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI1790194, Length=325, Percent_Identity=26.1538461538462, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994940, Length=330, Percent_Identity=27.8787878787879, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789068, Length=317, Percent_Identity=28.7066246056782, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=26.2658227848101, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788474, Length=261, Percent_Identity=30.2681992337165, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=28.719723183391, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786540, Length=343, Percent_Identity=26.530612244898, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=26.517571884984, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=23.8805970149254, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790689, Length=258, Percent_Identity=27.906976744186, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790715, Length=335, Percent_Identity=25.3731343283582, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786268, Length=187, Percent_Identity=24.0641711229947, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36652; Mature: 36521

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAAIEELGFVRNRQASALR
CCCCCEEEHHHHHHHCCCCEEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCHHHHC
GGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCC
VDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDTVRPDNMQAAQLLTEHLIRN
CCEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHC
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPV
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
TWASTPARELGITLADRMMQKITHEETHSRNLIIPARLIAAK
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCC
>Mature Secondary Structure 
ATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAAIEELGFVRNRQASALR
CCCCEEEHHHHHHHCCCCEEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCHHHHC
GGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCC
VDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDTVRPDNMQAAQLLTEHLIRN
CCEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHC
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPV
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
TWASTPARELGITLADRMMQKITHEETHSRNLIIPARLIAAK
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503 [H]