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The map label for this gene is pntA

Identifier: 30063117

GI number: 30063117

Start: 1690646

End: 1692193

Strand: Reverse

Name: pntA

Synonym: S1756

Alternate gene names: 30063117

Gene position: 1692193-1690646 (Counterclockwise)

Preceding gene: 30063121

Following gene: 30063116

Centisome position: 36.79

GC content: 52.26

Gene sequence:

>1548_bases
ATGGAAGGGAATATCATGCGAATTGGCATACCAAGAGAACGGTTAACCAATGAAACCCGTGTTGCAGCAACGCCAAAAAC
AGTGGAACAGCTGCTGAAACTGGGTTTTACCGTCGCGGTAGAGAGCGGCGCGGGTCAACTGGCAAGTTTTGACGATAAAG
CGTTTGTGCAAGCGGGCGCTGAAATTGTAGAAGGGAATAGCGTCTGGCAGTCAGAGATCATTCTGAAGGTCAATGCGCCG
TTAGATGATGAAATTGCGTTACTGAATCCCGGGACAACGCTGGTGAGTTTTATCTGGCCTGCGCAGAATCCGGAATTAAT
GCAAAAACTTGCGGAACGTAACGTGACCGTGATGGCGATGGACTCTGTGCCGCGTATCTCACGCGCACAATCGCTGGACG
CACTAAGCTCGATGGCGAATATCGCCGGTTATCGCGCCATTGTTGAAGCGGCACATGAATTTGGGCGCTTCTTTACCGGG
CAAATCACTGCGGCCGGGAAAGTGCCACCGGCAAAAGTGATGGTGATTGGTGCCGGTGTTGCAGGTTTGGCCGCCATTGG
CGCAGCAAACAGTCTCGGCGCGATTGTGCGTGCATTCGACACCCGCCCGGAAGTGAAAGAACAAGTTCAAAGTATGGGCG
CGGAATTCCTCGAGCTGGATTTTAAAGAGGAAGCGGGCAGCGGCGATGGCTATGCCAAAGTGATGTCGGACGCGTTCATC
AAAGCGGAAATGGAACTCTTTGCCGCCCAGGCAAAAGAGGTCGATATCATTGTCACCACCGCGCTTATTCCAGGCAAACC
AGCGCCGAAGCTAATTACCCGTGAAATGGTTGACTCCATGAAGGCGGGCAGTGTGATTGTCGACCTGGCAGCCCAAAACG
GTGGCAACTGTGAATACATCGTGCCGGGTGAAATCTTCAGTACGGAAAATGGTGTCAAAGTGATTGGTTATACCGATCTT
CCGGGCCGTCTGCCGACGCAATCCTCACAGCTTTACGGTACTAACCTCGTTAATCTGCTGAAACTGTTGTGCAAAGAGAA
AGACGGCAATATCACTGTTGATTTTGATGATGTGGTGATTCGCGGCGTGACCGTGATCCGTGCGGGCGAAATTACCTGGC
CGGCACCGCCGATTCAGGTATCAGCTCAGCCGCAGGCGGCACAAAAAGCGGCACCGGAAGTGAAAACTGAGGAAAAATGT
GCCTGCTCACCGTGGCGTAAATACGCGTTGATGGCGCTGGCAATCATCCTGTTCGGTTGGCTGTCAAGCGTTGCGCCGAA
AGAGTTTCTTGGGCACTTCACCGTTTTCGCGCTGGCCTGCGTTGTCGGTTATTACGTGGTGTGGAATGTATCGCACGCGC
TGCATACACCGTTGATGTCGGTTACCAACGCGATTTCAGGGATTATTGTTGTCGGAGCACTGTTGCAGATTGGCCAGGGA
GGCTGGGTTAGCTTCCTTAGTTTTATCGCGGTGCTTATAGCCAGCATTAATATTTTCGGTGGCTTCACCGTGACTCAGCG
CATGCTGAAAATGTTCCGCAAAAATTAA

Upstream 100 bases:

>100_bases
GGGCGTTTGTTACTAATTTATTTTAACGGAGTAACATTTAGCTCGTACATGAGCAGCTTGTGTGGCTCCTGACACAGGCA
AACCATCATCAATAAAACCG

Downstream 100 bases:

>100_bases
GGGGTAACATATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCGATCCTGTTTATCTTCAGTCTGGCCGGTC
TTTCGAAACATGAAACGTCT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MEGNIMRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAP
LDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFI
KAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKC
ACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQG
GWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_515_residues
MEGNIMRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAP
LDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFI
KAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKC
ACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQG
GWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_515_residues
MEGNIMRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAP
LDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTG
QITAAGKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFI
KAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDL
PGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKC
ACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQG
GWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=532, Percent_Identity=50.9398496240602, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI122939153, Length=532, Percent_Identity=50.9398496240602, Blast_Score=495, Evalue=1e-140,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=99.0196078431373, Blast_Score=1031, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=525, Percent_Identity=50.2857142857143, Blast_Score=481, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 55135; Mature: 55135

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGNIMRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGA
CCCCEEEECCCHHHHCCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHCH
EIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAM
HHCCCCCEEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCEEEEEE
DSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCH
AGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFI
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCCHHHHHHHHHH
KAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYI
HHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE
VPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVI
ECCCEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHH
RGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCACSPWRKYALMALAIILFGW
HCEEEEEECCEECCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEGNIMRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGA
CCCCEEEECCCHHHHCCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHCH
EIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAM
HHCCCCCEEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCEEEEEE
DSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCH
AGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFI
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCCHHHHHHHHHH
KAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYI
HHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE
VPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVI
ECCCEECCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHH
RGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCACSPWRKYALMALAIILFGW
HCEEEEEECCEECCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GWVSFLSFIAVLIASINIFGGFTVTQRMLKMFRKN
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]