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The map label for this gene is ynfL

Identifier: 30063110

GI number: 30063110

Start: 1682957

End: 1683868

Strand: Reverse

Name: ynfL

Synonym: S1748

Alternate gene names: 30063110

Gene position: 1683868-1682957 (Counterclockwise)

Preceding gene: 30063113

Following gene: 30063109

Centisome position: 36.61

GC content: 51.86

Gene sequence:

>912_bases
ATGAATATTGAACTTCGTCATCTGCGTTACTTTGTTGCTGTTGCGGAAGAGCTGCATTTTGGGCGCGCGGCTGCCCGCCT
GAATATTTCGCAACCGCCGCTAAGTCAGCAGATTCAGGCACTGGAGCAACAAATCGGTGCCCGACTGCTGGCACGAACTA
ATCGCAGTGTATTGCTGACGGCAGCAGGAAAACAGTTTCTTGCAGATAGTCGGCAAATCCTGTCTATGGTGGATGACGCT
GCCGCTCGCGCTGAAAGACTGCATCAGGGTGAAGCGGGGGAGTTGCGTATTGGTTTTACTTCGTCCGCTCCTTTTATTCG
GGCGGTGTCCGATACGTTATCGCTGTTTCGCCGTGATTATCCTGATGTCCATTTACAAACCCGCGAAATGAACACTCGCG
AGCAAATCGCTCCGCTCATTGAAGGAACGCTGGATATGGGATTGCTGCGTAACACAGCGTTACCGGAGACGCTTGAACAC
GCAGTCATCGTCCATGAACCGCTTATGGCGATGATCCCGTACGATCATCCCCTGGCAAATAATCCGAGTGTAACACTGGC
TGAACTGGCTGAACTGGCTGAACTGGCGAAAGAACCCTTTGTCTTTTTTGATCCGCACGTCGGGACTGGGCTGTATGACG
ATATTCTCGGACTGATGCGACGTTACCATTTGACGCCCGTCATCACTCAGGAGGTGGGCGAGGCAATGACCATCATCGGT
CTGGTTTCCGCCGGTCTGGGTGTTTCAATTTTGCCTGCGTCATTTAAACGTGTTCAGCTCAACGAAATGCGCTGGGTGCC
GATTGCTGAAGAGGATGCGGTTTCTGAAATGTGGTTGGTCTGGCCGAAACATCATGAACAAAGTCCAGCTGCGCGTAACT
TTCGTATTCATCTGCTGAATGCTCTCAGGTGA

Upstream 100 bases:

>100_bases
TCCTTGCTAAATATGCCTGTGGATCAGGCTTATACATAGGGTAGGAAAATCGAATTGTTCTGTCTAATATATTAATAATC
TCAAATAAGATGTTTTAAAT

Downstream 100 bases:

>100_bases
GGGAAATTTCAGCGAAAAAGCCCGAAAAATGTGCTGTTAATCACATGCCTAAGTAAAAATTTGACGACACGTATTGAAGT
GCTTCACCATAGCCTACAGA

Product: putative LYSR-type transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIG
LVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR

Sequences:

>Translated_303_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIG
LVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
>Mature_303_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIG
LVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=303, Percent_Identity=97.029702970297, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=35.6164383561644, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI2367136, Length=263, Percent_Identity=37.2623574144487, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790399, Length=254, Percent_Identity=31.1023622047244, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693105, Length=301, Percent_Identity=26.578073089701, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788748, Length=253, Percent_Identity=28.4584980237154, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI157672245, Length=298, Percent_Identity=29.1946308724832, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081904, Length=191, Percent_Identity=32.4607329842932, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=27.906976744186, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790208, Length=255, Percent_Identity=28.6274509803922, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786713, Length=175, Percent_Identity=30.8571428571429, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787530, Length=275, Percent_Identity=26.1818181818182, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=94, Percent_Identity=43.6170212765958, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789506, Length=287, Percent_Identity=23.3449477351916, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787806, Length=291, Percent_Identity=23.7113402061856, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=261, Percent_Identity=25.2873563218391, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=307, Percent_Identity=24.1042345276873, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=32.3076923076923, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=163, Percent_Identity=32.5153374233129, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082132, Length=305, Percent_Identity=24.2622950819672, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33922; Mature: 33922

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50931 HTH_LYSR ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPYDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCC
NPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIG
CCCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLN
HHHCCCCCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCCEEEEEH
ALR
HCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPYDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCC
NPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIG
CCCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLN
HHHCCCCCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCCEEEEEH
ALR
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]