Click here to switch to the map view.

The map label for this gene is dadX

Identifier: 30062707

GI number: 30062707

Start: 1225471

End: 1226541

Strand: Direct

Name: dadX

Synonym: S1267

Alternate gene names: 30062707

Gene position: 1225471-1226541 (Clockwise)

Preceding gene: 30062706

Following gene: 30062711

Centisome position: 26.64

GC content: 57.7

Gene sequence:

>1071_bases
ATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCCGCGCCGCA
CGCGCGCGTCTGGTCGGTGGTAAAAGCGAACGCTTACGGGCATGGTATTGAGCGTATCTGGAGCGCGCTCGGGGCCACCG
ATGGCTTTGCATTACTTAACCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAGGGGCCGATCCTGATGCTGGAA
GGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAGCTCAA
AGCACTGCAAAATGCGCGGCTAAAAGCACCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCC
AGTCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCAATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTT
GCTGAAGCGGAACATCCTGATGGAATTTCCGGCGCGATGGCGCGTATTGAGCAGGCGGCGGAGGGGCTGGAGTGTCGGCG
TTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATG
GCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATT
GGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCAT
TGTCGCCGCAGGATACGCCGACGGTTATCCGCGCCATGCGCCTACCGGTGCTCCTGTTTTAGTGGACGGCGTGCGCACCA
TGACGGTGGGGACCGTCTCGATGGATATGCTGGCGGTTGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGTT
GAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGATGTGCGCACT
GGCGTTACGCGTCCCGGTTGTGACAGTGTAA

Upstream 100 bases:

>100_bases
AGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCG
CACACAGCTAAGGAAACGAG

Downstream 100 bases:

>100_bases
CTTGTTGTAAGCCGGATCGGAGGCAGCGTCTTCTGGTTGCGAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTGT
TAATGTGACAGAGCCATTGC

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Sequences:

>Translated_356_residues
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
>Mature_355_residues
TRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLEG
FFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHFA
EAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEIIG
VQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVE
LWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=356, Percent_Identity=98.876404494382, Blast_Score=723, Evalue=0.0,
Organism=Escherichia coli, GI1790487, Length=346, Percent_Identity=42.1965317919075, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 38801; Mature: 38670

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLN
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
YLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
HHHHHHHHHCCCCEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGAPVLVDGVRTMTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure 
TRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLN
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEE
YLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
HHHHHHHHHCCCCEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGAPVLVDGVRTMTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]