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The map label for this gene is insF1

Identifier: 30062696

GI number: 30062696

Start: 1213072

End: 1213926

Strand: Direct

Name: insF1

Synonym: S1254

Alternate gene names: 30062696

Gene position: 1213072-1213926 (Clockwise)

Preceding gene: 30062695

Following gene: 30062697

Centisome position: 26.37

GC content: 55.2

Gene sequence:

>855_bases
ATGAAGTATGTCTTTATTGAAAAACATCAGGCTGAGTTCAGCATCAAAGCAATGTGCCGCGTGCTCCGGGTGGCCCGCAG
CGGCTGGTATACGTGGTGTCAGCGGCGGACAAGGATAAGCACGCGTCAGCAGTTCCGCCAACACTGCGACAGCGTTGTCC
TCGCGGCTTTTACCCGGTCAAAACAGCGTTACGGTGCCCCACGCCTGACGGATGAACTGCGTGCTCAGGGTTACCCCTTT
AACGTAAAAACCGTGGCGGCAAGCCTGCGCCGTCAGGGACTGAGGGCAAAGGCCTCCCGGAAGTTCAGCCCGGTCAGCTA
CCGCGCACACGGCCTGCCTGTGTCAGAAAATCTGTTGGAGCAGGATTTTTACGCCAGTGGCCCGAACCAGAAGTGGGCAG
GAGACATCACGTACTTACGTACAGATGAAGGCTGGCTGTATCTGGCAGTGGTCATTGACCTGTGGTCACGTGCCGTTATT
GGCTGGTCAATGTCGCCACGCATGACGGCGCAACTGGCCTGCGATGCCCTGCAGATGGCGCTGTGGCGGCGTAAGAGGCC
CCGGAACGTTATCGTTCACACGGACCGTGGAGGCCAGTACTGTTCAGCAGATTATCAGGCGCAACTGAAGCGGCATAATC
TGCGTGGAAGTATGAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAA
TGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCG
GTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACTAG

Upstream 100 bases:

>100_bases
TGTCTACCGAGATTGCACGTCTCAAACGCCAGCTGGCAGAACGGGATGAAGAGCTGGCTATCCTCCAAAAGGCCGCGACA
TACTTCGCGAAGCGCCTGAA

Downstream 100 bases:

>100_bases
AACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACTATGGCCTTTGCTGAACTCAACATTGACATTCTTACC
CGCAGCAAAGCTCGTGGAGG

Product: IS3 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFEN

Sequences:

>Translated_284_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFEN
>Mature_284_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFEN

Specific function: Involved in the transposition of the insertion sequence IS3

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain

Homologues:

Organism=Homo sapiens, GI310129419, Length=242, Percent_Identity=33.8842975206612, Blast_Score=147, Evalue=8e-36,
Organism=Escherichia coli, GI1788405, Length=284, Percent_Identity=100, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI1787263, Length=284, Percent_Identity=100, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI1786753, Length=284, Percent_Identity=100, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI1786570, Length=284, Percent_Identity=100, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI1786490, Length=284, Percent_Identity=100, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI1789981, Length=272, Percent_Identity=30.8823529411765, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): INSF1_ECOLI (P0CF79)

Other databases:

- EMBL:   X02311
- EMBL:   U73857
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64756
- RefSeq:   AP_000954.1
- RefSeq:   AP_001023.1
- RefSeq:   AP_001186.1
- RefSeq:   AP_001655.1
- RefSeq:   AP_002686.1
- RefSeq:   NP_414833.1
- RefSeq:   NP_414906.1
- RefSeq:   NP_415073.1
- RefSeq:   NP_415545.1
- RefSeq:   NP_416593.1
- ProteinModelPortal:   P0CF79
- SMR:   P0CF79
- EnsemblBacteria:   EBESCT00000004084
- EnsemblBacteria:   EBESCT00000015377
- GeneID:   944948
- GeneID:   945036
- GeneID:   945587
- GeneID:   946629
- GeneID:   947988
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0293
- KEGG:   eco:b0299
- EchoBASE:   EB4710
- EcoGene:   EG40006
- eggNOG:   COG2801
- GeneTree:   EBGT00070000031687
- HOGENOM:   HBG757639
- GO:   GO:0032196
- InterPro:   IPR001584
- InterPro:   IPR012337

Pfam domain/function: PF00665 rve; SSF53098 RNaseH_fold

EC number: NA

Molecular weight: Translated: 33114; Mature: 33114

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRS
CCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLE
HHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCEEECCCCHHHHHHH
QDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMA
HHHHCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
HHHCCCCCEEEEEECCCCCEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFEN
EECCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRS
CCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLE
HHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCEEECCCCHHHHHHH
QDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMA
HHHHCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
HHHCCCCCEEEEEECCCCCEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFEN
EECCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503