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The map label for this gene is thiK

Identifier: 30062639

GI number: 30062639

Start: 1153870

End: 1154694

Strand: Direct

Name: thiK

Synonym: S1190

Alternate gene names: 30062639

Gene position: 1153870-1154694 (Clockwise)

Preceding gene: 30062638

Following gene: 30062640

Centisome position: 25.09

GC content: 52.73

Gene sequence:

>825_bases
GTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTTTCATCCCGTCACGAC
GTTTAATAGTGGGCTTAGTGGCGGTAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATC
CTGATGCGCCGCAGTCCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCAAGCATTGCACCGAAGCCGCAT
TTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGTGCGGTAAAAACGTATTTGCCGGATACCAACGAACTGGC
AGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCCTTACTGGAACTGTACTGGC
AGCAAAGCGATCCGGCGCGGCGGACTGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCAAAGCGCGGGAACCACGACCT
TTACGCTTAAGTCCATTGCATATGGATGTCCACGCCGGAAATTTAGTGCATAGCGCATCAGGGTTAAAACTCATCGACTG
GGAGTATGCTGGAGATGGTGATATCGCGCTGGAACTGGCGGCGGTGTGGGTGGAAAATACTGAACAGCACCGGCAATTGG
TCAATGACTATGCCACTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTG
ATGCTCAAAGGAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGATGACACCTG
GCGGCAGCTATTAATAAAACAATAA

Upstream 100 bases:

>100_bases
TACGTGCTGTACTCCTGCGCCTCTGGCAACGTTAACGCTCCGACCCTACAAATGCAGCTGATGCTGGTGCAGACGGGCGA
AATTATCTGGTCAGGTAAAG

Downstream 100 bases:

>100_bases
GGAGAGCAGTGTGGGTCCAGTAATGTTGGATGTCGAAGGTTACGAACTGGACGCGGAAGAGCGTGAAATACTGGCGCATC
CGCTGGTGGGAGGGCTGATT

Product: thiamine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPH
LYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
MLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ

Sequences:

>Translated_274_residues
MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPH
LYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
MLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
>Mature_273_residues
PFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHL
YLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPL
RLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLLM
LKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate [H]

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787349, Length=274, Percent_Identity=98.9051094890511, Blast_Score=553, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.89 [H]

Molecular weight: Translated: 32352; Mature: 32220

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSA
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHH
FLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFG
HHHHHHHHHHCCHHCCCCCCEEEEEEEHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCCC
WRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSAS
EEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEEECCC
GLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
CCEEEEEEECCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHH
FLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFG
HHHHHHHHHHCCHHCCCCCCEEEEEEEHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCCC
WRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSAS
EEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEEECCC
GLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
CCEEEEEEECCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA