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The map label for this gene is rutB [H]

Identifier: 30062548

GI number: 30062548

Start: 1064463

End: 1065197

Strand: Reverse

Name: rutB [H]

Synonym: S1084

Alternate gene names: 30062548

Gene position: 1065197-1064463 (Counterclockwise)

Preceding gene: 30062549

Following gene: 30062547

Centisome position: 23.16

GC content: 55.78

Gene sequence:

>735_bases
GTGCCGCGCCCATCTCTCTGCGCTGACTCAGGAGGTGGCATGATGACAACCTTAACCGCTCGACCGGAAGCCATTACCTT
CGATCCGCAGCAAAGTGCGCAGATCGTGGTGGATATGCAAAACGCTTATGCCACGCCAGGCGGCTACTTAGATCTCGCCG
GGTTTGATGTCTCAACCACTCGCCCGGTCATTGCCAACATTCAAACCGCTGTGACCGCAGCGCGAGCGGCAGGGATGTTG
ATCATCTGGTTTCAAAATGGCTGGGATGAACAGTATGTCGAGGCTGGCGGCCCCGGCTCACCGAATTTTCATAAATCGAA
CGCCCTGAAAACCATGCGTAAGCAGCCGCAGCTGCAGGGGAAATTGCTGGCGAAAGGCTCCTGGGATTATCAACTGGTGG
ATGAACTGGTGCCGCAGCCTGGCGATATTGTGCTGCCGAAGCCGCGCTACAGCGGTTTCTTCAATACGCCGCTGGACAGC
ATTTTACGCAGCCGTGGAATACGCCATCTGGTTTTCACCGGTATCGCTACCAACGTCTGCGTCGAATCGACGCTACGCGA
TGGCTTTTTTCTGGAGCATTTCGGCGTGGTGCTGGAAGACGCAACACACCAGGCGGGGCCGGAATTTGCACAGAAAGCCG
CGTTGTTCAATATCGAAACCTTTTTTGGCTGGGTCAGCGACGTCGAAACGTTCTGCGACGCGCTTTCTCCCACGTCCTTT
GCTCGTATCGCTTAA

Upstream 100 bases:

>100_bases
AAGTCGCAAGCGTGCCTGGTGCAGAAGGCGTGCTGTTGACCTTCGATGATTTTCTGTCGGGAATCGAAACCTTCGGCGAG
CGCATTCAACCACTGATGCA

Downstream 100 bases:

>100_bases
GGAGTTTAGCGATGCCAAAATCCGTAATTATTCCCGCTGGCAGCAGCGCACCGCTGGCCCCCTTCGTTCCCGGCACGCTG
GCTGATGGCGTGGTGTATGT

Product: putative synthetase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MPRPSLCADSGGGMMTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGML
IIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDS
ILRSRGIRHLVFTGIATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSF
ARIA

Sequences:

>Translated_244_residues
MPRPSLCADSGGGMMTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGML
IIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDS
ILRSRGIRHLVFTGIATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSF
ARIA
>Mature_243_residues
PRPSLCADSGGGMMTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLI
IWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSI
LRSRGIRHLVFTGIATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFA
RIA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=230, Percent_Identity=98.2608695652174, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 26578; Mature: 26447

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPSLCADSGGGMMTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVSTT
CCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCCC
RPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQG
CHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC
KLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVC
EEEECCCCCHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHH
VESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSF
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCH
ARIA
HHCC
>Mature Secondary Structure 
PRPSLCADSGGGMMTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVSTT
CCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCCC
RPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQG
CHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC
KLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVC
EEEECCCCCHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHH
VESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSF
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCH
ARIA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA