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The map label for this gene is rutF [H]

Identifier: 30062544

GI number: 30062544

Start: 1062152

End: 1062610

Strand: Reverse

Name: rutF [H]

Synonym: S1080

Alternate gene names: 30062544

Gene position: 1062610-1062152 (Counterclockwise)

Preceding gene: 30062545

Following gene: 30062543

Centisome position: 23.1

GC content: 60.35

Gene sequence:

>459_bases
ATGTCCTGTATGGGCGCGGCGGTCAATATCATCACCACGGACGGTCCAGCCGGGCGCGCCGGGTTCACCGCCAGCGCCGT
CTGCAGCGTGACCGACACGCCGCCCACATTACTGGTGTGCCTGAATCGTGGGGCGTCCGTCTGGCCGGTATTCAATGAAA
ATCGAACGCTGTGTGTAAATACACTGAGCGCCGGGCAGGAGCCGCTTTCAAATCTTTTTGGCGGCAAAACGCCCATGGAA
CACCGCTTTGCCGCCGCCCGCTGGCAGACGGGTGTGACCGGATGCCCGCAACTGGAAGAGGCTCTGGTTTCGTTTGACTG
CCGTATCAGCCAGGTGGTGAGCGTCGGCACCCACGACATTCTGTTTTGCGCCATCGAAGCGATTCATCGTCACACCACAC
CCTACGGGCTGGTGTGGTTCGATCGCAGTTATCACGCGCTGATGCGCCCTGCTTGTTAA

Upstream 100 bases:

>100_bases
CTTTTTGCGCGCCTGCCACGTCTGTCCTTTGAAGAAGCCTGCGGGCTGTTGTAAGGAGCAAACTATGAACATAGTTGATC
AACAAACCTTTCGCGATGCG

Downstream 100 bases:

>100_bases
CCTCCTTAAGGAGACAGCTGATGGCAATGTTCGGTTTTCCTCACTGGCAGTTAAAATCGACCTCTACAGAGAGCGGCGTG
GTCGCGCCGGATGAACGATT

Product: hypothetical protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPME
HRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALMRPAC

Sequences:

>Translated_152_residues
MSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPME
HRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALMRPAC
>Mature_151_residues
SCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEH
RFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALMRPAC

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=152, Percent_Identity=99.3421052631579, Blast_Score=315, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 16358; Mature: 16227

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPVFNENRTLCVN
CCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCEEEEEECCCCEEEEE
TLSAGQEPLSNLFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDI
ECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHCCCHHH
LFCAIEAIHRHTTPYGLVWFDRSYHALMRPAC
HHHHHHHHHHCCCCCEEEEECCCHHHHCCCCC
>Mature Secondary Structure 
SCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPVFNENRTLCVN
CCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCEEEEEECCCCEEEEE
TLSAGQEPLSNLFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDI
ECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHCCCHHH
LFCAIEAIHRHTTPYGLVWFDRSYHALMRPAC
HHHHHHHHHHCCCCCEEEEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA