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The map label for this gene is rlmI [H]

Identifier: 30062506

GI number: 30062506

Start: 1020663

End: 1021766

Strand: Reverse

Name: rlmI [H]

Synonym: S1035

Alternate gene names: 30062506

Gene position: 1021766-1020663 (Counterclockwise)

Preceding gene: 161486514

Following gene: 30062505

Centisome position: 22.22

GC content: 51.81

Gene sequence:

>1104_bases
ATGGAAGGTAAAGCCAGCCTCGGTGAAACCATCGATATTGTTGATCATCAGGGAAAATGGTTAGCACGCGGCGCTTATTC
GCCAGCTTCGCAAATCCGGGCGCGCGTCTGGACGTTTGACCCGTCTGAGTCTATCGACATTGCTTTTTTTTCCCGCCGTT
TGCAACAAGCACAAAAATGGCGTGACTGGCTGGCGCAAAAAGATGGCCTCGACAGCTATCGTTTAATCGCCGGAGAATCT
GATGGCCTGCCGGGTATTACTATCGATCGTTTCGGTAATTTTCTGGTGCTGCAACTGCTGAGTGCTGGCGCAGAATATCA
GCGCGCGGCATTAATTAGTGCCCTGCAAACGCTGTACCCGGAATGTGCGATTTACGATCGCAGCGATGTTGCGGTACGTA
AAAAAGAAGGGATGGAGCTGACCCAGGGCCTCGTCACCGGCGAGTTGCCGCCTGCCCTGCTGCCGATTGAAGAACACGGC
ATGAAGCTGCTGGTGGATATTCAGCACGGACACAAAACGGGCTACTATCTGGACCAGCGAGACAGCCGCCTGGCTACCCG
CCGCTACGTTGAAAATAAACGTGTGCTGAACTGTTTCTCCTATACCGGTGGTTTCGCCGTATCGGCACTGATGGGCGGTT
GCAGCCAGGTTGTCAGCGTTGATACCTCCCAGGAAGCGCTGGATATTGCACGGCAGAACGTTGAGCTGAACAAACTGGAT
CTGAGCAAGGCTGAGTTTGTCCGTGATGATGTCTTTAAATTGCTGCGTACTTATCGCGATCGCGGTGAAAAATTTGACGT
TATCGTGATGGACCCGCCGAAGTTTGTTGAGAATAAAAGCCAGTTGATGGGCGCGTGTCGTGGTTATAAAGACATCAACA
TGCTGGCGATTCAGTTGCTGAATGAAGGCGGTATTCTCCTGACTTTCTCCTGTTCCGGTCTGATGACCAGCGATTTATTT
CAGAAAATCATCGCGGATGCCGCAATTGATGCCGGTCGTGATGTACAATTTATAGAGCAGTTCCGTCAGGCAGCCGATCA
TCCGGTGATCGCTACCTACCCGGAAGGGCTATATCTGAAAGGGTTTGCCTGTCGCGTCATGTAA

Upstream 100 bases:

>100_bases
AGAAAAGTAAATTATGAGTGTACGTTTAGTGTTAGCCAAAGGGCGCGAAAAATCATTACTTCGTCGCCATCCGTGGGTCT
TTTCCGGGGCCGTTGCCCGC

Downstream 100 bases:

>100_bases
CTTGAAATGTGGAATTTTACCCTTATATCAAAGGTAAAGATTTCCCGGGAGGTGACTATGATTGCCAGCAAATTCGGTAT
CGGCCAGCAGGTCCGCCATT

Product: putative oxidoreductase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGLVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM

Sequences:

>Translated_367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGLVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
>Mature_367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGLVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=367, Percent_Identity=99.4550408719346, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=241, Percent_Identity=29.4605809128631, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 41054; Mature: 41054

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKW
CCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHH
RDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYP
HHHHHHCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
ECAIYDRSDVAVRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQR
CCEEECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEECCCCCCCEECCC
DSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
CHHHHHHHHHHCCHHEEHHHCCCHHHHHHHHHCHHHHHCCCCCHHHHHHHHHCCCCCEEC
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLL
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHHHHHCCCCHHHHHHHHHH
NEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLK
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEC
GFACRVM
CEEEECC
>Mature Secondary Structure
MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKW
CCCCCCCCCEEEEECCCCCEEECCCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHH
RDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYP
HHHHHHCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
ECAIYDRSDVAVRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQR
CCEEECCCCCHHHHHCCHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEECCCCCCCEECCC
DSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
CHHHHHHHHHHCCHHEEHHHCCCHHHHHHHHHCHHHHHCCCCCHHHHHHHHHCCCCCEEC
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLL
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHHHHHCCCCHHHHHHHHHH
NEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLK
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEC
GFACRVM
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA