Definition Shigella flexneri 2a str. 2457T, complete genome.
Accession NC_004741
Length 4,599,354

Click here to switch to the map view.

The map label for this gene is rmf

Identifier: 30062490

GI number: 30062490

Start: 1006747

End: 1006914

Strand: Direct

Name: rmf

Synonym: S1019

Alternate gene names: 30062490

Gene position: 1006747-1006914 (Clockwise)

Preceding gene: 30062489

Following gene: 30062493

Centisome position: 21.89

GC content: 54.76

Gene sequence:

>168_bases
ATGAAGAGACAAAAACGAGATCGCCTGGAACGGGCACATCAACGTGGTTATCAGGCCGGCATCGCCGGACGCTCAAAAGA
AATGTGTCCCTATCAGACGCTGAGTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAA
TGGCCTGA

Upstream 100 bases:

>100_bases
TTGACGGCAGTTATGATTCGCGGTATTGCTTAACTGTGATTGCACATTTAGTAATCACTGTTTTCTTTTCCACCAGAAAC
CAGTATGAGGGAAACGAGGC

Downstream 100 bases:

>100_bases
TTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATGATAATCAGAAGGCAG
ACGTATCCTGGAACAGACCG

Product: ribosome modulation factor

Products: NA

Alternate protein names: Protein E [H]

Number of amino acids: Translated: 55; Mature: 55

Protein sequence:

>55_residues
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGWREAMADRVVMA

Sequences:

>Translated_55_residues
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGWREAMADRVVMA
>Mature_55_residues
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGWREAMADRVVMA

Specific function: Associates with 70S ribosomes and converts them to a dimeric form (100S ribosomes) which appears during the transition from the exponential growth phase to the stationary phase of E.coli cells [H]

COG id: COG3130

COG function: function code J; Ribosome modulation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787186, Length=55, Percent_Identity=98.1818181818182, Blast_Score=112, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007040
- InterPro:   IPR023200
- ProDom:   PD051650 [H]

Pfam domain/function: PF04957 RMF [H]

EC number: NA

Molecular weight: Translated: 6481; Mature: 6481

Theoretical pI: Translated: 11.41; Mature: 11.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
7.3 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
7.3 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGWREAMADRVVMA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGWREAMADRVVMA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]