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The map label for this gene is ycbF

Identifier: 30062481

GI number: 30062481

Start: 994951

End: 995688

Strand: Direct

Name: ycbF

Synonym: S1010

Alternate gene names: 30062481

Gene position: 994951-995688 (Clockwise)

Preceding gene: 30062480

Following gene: 30062482

Centisome position: 21.63

GC content: 50.95

Gene sequence:

>738_bases
ATGCCAGCGCGACATTTGTACTTCATTATGACTAATACGTGGAACAGATTAGCGCTCTTGATTTTCGCCGTTTTATCGCT
GCTGGTGGCGGGTGAATTGCAGGCAGGAGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATAT
CTATTTTACTGACTAATACCAGTCAGGAATCTTGGCTTATTAACAGCAAAATCAATCGCCCAACGCGTTGGGCCGGGGGG
GAAGCGTCGACAGTGCCAGCACTATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACTACAGGCACGTTGCG
CTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAACGCTATTTGAGTTAAGCATTGCCAGCGTGCCATCCG
GCAAAGTTGAAAATCAGAGCGTAAAAGTGGCGATGCGCTCGGTATTTAAACTGTTCTGGCGACCCGAAGGTTTGCCGGGC
GACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTACTA
CATTAACCTGATTCAGGTGAGCGTAAATGGTAAAGCGTTAAGTAATGCGGGAGTCGTGCCGCCTAAAAGCCAGCGTCAGA
CAAACTGGTGTCAGGTAATAGCACCGTGTCATGTCGCCTGGCGGACGATAAATGATTACGGCGGGTTGAGTGCAAAGAAG
GAGCAAAATCTGCCCTGA

Upstream 100 bases:

>100_bases
CAGGCGAGTAAAGACTACAGCCTACATAGCGGTAAGGTGCCATTAACTTTTTATGCGCAATTGCGACCTGTCAATAGCGA
TGTGCAGTCGGGTAAAGTGA

Downstream 100 bases:

>100_bases
AACAGGTTCTGAAAACGTTTGCGTTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCC
CGGTTTGCACACCGGGAATC

Product: putativi pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNTSQESWLINSKINRPTRWAGG
EASTVPALLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQVIAPCHVAWRTINDYGGLSAKK
EQNLP

Sequences:

>Translated_245_residues
MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNTSQESWLINSKINRPTRWAGG
EASTVPALLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQVIAPCHVAWRTINDYGGLSAKK
EQNLP
>Mature_244_residues
PARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNTSQESWLINSKINRPTRWAGGE
ASTVPALLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGD
PLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQVIAPCHVAWRTINDYGGLSAKKE
QNLP

Specific function: Could be required for the biogenesis of a putative fimbria [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI87081806, Length=236, Percent_Identity=97.8813559322034, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790771, Length=223, Percent_Identity=33.1838565022422, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787171, Length=222, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786743, Length=240, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786333, Length=242, Percent_Identity=30.9917355371901, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789532, Length=228, Percent_Identity=26.7543859649123, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082203, Length=216, Percent_Identity=26.3888888888889, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786936, Length=184, Percent_Identity=32.6086956521739, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788428, Length=220, Percent_Identity=25.9090909090909, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788677, Length=176, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 27056; Mature: 26924

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNT
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCEEEEEEECC
SQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGTTGTLRLLRTESDILPVDR
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCEEEEEECCCCCCCCCH
ETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQGVQL
HHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEE
TNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQVIAPCHVAWRTINDYGGLSAKK
CCCCCEEEEEEEEEECCCEECCCCCCCCCCCCCCCCEEEEECHHEEEEEHHCCCCCCCCC
EQNLP
CCCCC
>Mature Secondary Structure 
PARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRFIFPADRESISILLTNT
CCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCEEEEEEECC
SQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGTTGTLRLLRTESDILPVDR
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCEEEEEECCCCCCCCCH
ETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQGVQL
HHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEE
TNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQVIAPCHVAWRTINDYGGLSAKK
CCCCCEEEEEEEEEECCCEECCCCCCCCCCCCCCCCEEEEECHHEEEEEHHCCCCCCCCC
EQNLP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2992959; 7984428 [H]