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The map label for this gene is focA

Identifier: 30062439

GI number: 30062439

Start: 939996

End: 940853

Strand: Reverse

Name: focA

Synonym: S0963

Alternate gene names: 30062439

Gene position: 940853-939996 (Counterclockwise)

Preceding gene: 30062440

Following gene: 30062438

Centisome position: 20.46

GC content: 48.6

Gene sequence:

>858_bases
GTGAAAGCTGACAACCCTTTTGATCTTTTACTTCCTGCTGCAATGGCCAAAGTGGCCGAAGAGGCGGGTGTCTATAAAGC
AACGAAACATCCGCTTAAGACTTTCTATCTGGCGATTACCGCCGGTGTTTTCATCTCAATCGCATTCGTCTTCTATATCA
CAGCAACCACTGGCACAGGCACAATGCCCTTCGGCATGGCAAAACTGGTTGGCGGCATTTGCTTCTCTCTGGGGCTGATT
CTTTGTGTTGTCTGCGGAGCCGATCTCTTTACTTCCACAGTGTTGATTGTTGTTGCTAAGGCGAGTGGGCGCATCACCTG
GTGTCAGTTGGCTAAAAACTGGCTAAATGTCTATTTTGGCAACCTGGTCGGCGCACTGCTGTTTGTACTTTTAATGTGGC
TTTCCGGCGAGTATATGACCGCAAATGGTCAATGGGGACTAAACGTCCTACAAACCGCCGACCACAAAGTGCACCATACT
TTTATTGAGGCCGTCTGTCTTGGTATCCTGGCAAACCTGATGGTATGTCTGGCAGTATGGATGAGTTATTCTGGCCGCAG
CCTGATGGACAAAGCGTTCATTATGGTGCTGCCGGTCGCGATGTTTGTTGCCAGCGGTTTTGAGCACAGTATCGCAAACA
TGTTTATGATCCCGATGGGTATTGTAATCCGCGACTTCGCATCTCCGGAATTCTGGACCGCTGTCGGTTCTGCACCGGAA
AATTTTTCTCACCTGACCGTGATGAACTTCATCACTGATAACCTGATCCCGGTTACGATCGGTAATATTATCGGCGGTGG
TTTGTTGGTTGGGTTGACATACTGGGTCATTTACCTGCGTGAAAACGATCACCATTAA

Upstream 100 bases:

>100_bases
TTACGGGCCTATAAGCCAGGCGAGATATGATCTATATCAATTTCTCATCTATAATGCTTTGTTAGTATCTCGTCGCCGAC
TTAATAAAGAGAGAGTTAGT

Downstream 100 bases:

>100_bases
TGGTTGTCGAAGTACGCAGTAAATAAAAAATCCACTTAAGAAGGTAGGTGTTACATGTCCGAGCTTAATGAAAAGTTAGC
CACAGCCTGGGAAGGTTTTA

Product: formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH

Sequences:

>Translated_285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
>Mature_285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=285, Percent_Identity=99.6491228070175, Blast_Score=575, Evalue=1e-166,
Organism=Escherichia coli, GI1788837, Length=282, Percent_Identity=50.354609929078, Blast_Score=280, Evalue=9e-77,
Organism=Escherichia coli, GI87082249, Length=272, Percent_Identity=29.0441176470588, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321779, Length=207, Percent_Identity=29.4685990338164, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 31037; Mature: 31037

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKASGRITWCQLAKNWLNVYFG
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHH
NLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
HCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKASGRITWCQLAKNWLNVYFG
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHH
NLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
HCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]