Click here to switch to the map view.

The map label for this gene is recE [H]

Identifier: 30062410

GI number: 30062410

Start: 910640

End: 911284

Strand: Reverse

Name: recE [H]

Synonym: S0929

Alternate gene names: 30062410

Gene position: 911284-910640 (Counterclockwise)

Preceding gene: 30062411

Following gene: 161486520

Centisome position: 19.81

GC content: 49.92

Gene sequence:

>645_bases
ATGAGCACAAAACCACTCTTCCTGTTACGGAAAGCGAAAAAATCATCCGGTGAACCTGACGTCGTCCTGTGGGCAAGCGA
CGATTTTGAATCGACCAGCACCACTCTGGACTATCTGCTCGTTAAGTCAGGTAAAAAACTGAGCAACTATTTTAAAGCTG
TTGCCACAAATTTTCCTGTCGTTAATGACCTTCCCCCTGAAGGTGAGATCGATTTTACCTGGAGTGAACGCTATCAACTC
AGCAAAGACTCCATGACATGGGAACTAAAACCGGGAGCAGCGCCAGACGACGTTCACCACCAGGATAATGCTCAAGAAAC
CAAAGAACTGGCGGGAGGCCAGGAAGAAAACGCGCAGGCAGACGCCCACGAGGATTGCCAGGATTGCGAAGTCTCTGTAG
CCACTTTGCGGTTCACACAGCGTCTTCTGCACATTTTTACGTATGCTGCCGGGGATCTGAAATACCTGCATCATGCCACC
CGTGAACAACGCAAACACATTACTGCTCTTGAGATGGATCAGGAAAACAGCTATGTCCAGAATCTGCTGTTGGCCATACG
CGGCATGGCAGAACCGACAACTCTGGATAATGCCGCCCTGCTCCGCCTGACTGATGCAATTAAGGCTGAGGTGTACTGGC
AATAG

Upstream 100 bases:

>100_bases
AAAAAAAGAAAGCCTGCACAGTGTGCAGGCCTGAGTGAAGAACCTGGGACATTTATTCATCACTCGCAGTAATTTTAATC
TGAGTTGAGGTTAAAAAACA

Downstream 100 bases:

>100_bases
CGGACACTACCATTTGTTCTTTTTTTAAGCAGCCATCTGATGATATTTTTCCCTGAAGGCTGCCGGGGAGATATTCCCCA
GACGAGAGTGACGACGCTGA

Product: putative exodeoxyribonuclease VIII of prophage CP-933R

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSGKKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQL
SKDSMTWELKPGAAPDDVHHQDNAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAEVYWQ

Sequences:

>Translated_214_residues
MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSGKKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQL
SKDSMTWELKPGAAPDDVHHQDNAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAEVYWQ
>Mature_213_residues
STKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSGKKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLS
KDSMTWELKPGAAPDDVHHQDNAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHATR
EQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAEVYWQ

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=117, Percent_Identity=82.9059829059829, Blast_Score=200, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 24176; Mature: 24045

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSGKKLSNYFKAVATNFPV
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
VNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQDNAQETKELAGGQEENAQA
CCCCCCCCCEEEEECCCEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
DAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHATREQRKHITALEMDQENSYVQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHH
NLLLAIRGMAEPTTLDNAALLRLTDAIKAEVYWQ
HHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
STKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSGKKLSNYFKAVATNFPV
CCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
VNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQDNAQETKELAGGQEENAQA
CCCCCCCCCEEEEECCCEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
DAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHATREQRKHITALEMDQENSYVQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHH
NLLLAIRGMAEPTTLDNAALLRLTDAIKAEVYWQ
HHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]