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The map label for this gene is ybiY

Identifier: 30062307

GI number: 30062307

Start: 802830

End: 803756

Strand: Reverse

Name: ybiY

Synonym: S0817

Alternate gene names: 30062307

Gene position: 803756-802830 (Counterclockwise)

Preceding gene: 30062309

Following gene: 30062306

Centisome position: 17.48

GC content: 54.26

Gene sequence:

>927_bases
ATGTTTGAACGAAACAGAGAGGCAACTATGATTTTCAATATTCAGCGCTACTCGACCCATGATGGCCCCGGTATCCGCAC
GGTCGTATTTCTTAAAGGCTGTTCGCTGGGCTGCCGCTGGTGTCAGAACCCGGAAAGCCGCGCCCGTACGCAGGATCTGC
TGTATGACGCACGACTGTGTCTGGAAGGCTGCGAGCTGTGCGCTAAGGCCGCGCCGGAAGTGATTGAGCGCGCGCTGAAT
GGTTTGCTTATTCATCGGGAAAAGTTAACCCCGGAGCATCTGACGGCGTTAACCGACTGCTGTCCGACACAGGCGTTAAC
CGTGTGTGGTGAAGTGAAAAGCGTTGAGGAGATCATGGCGACCGTTCTGCGCGATAAACCGTTTTACGATCGCAGCGGCG
GCGGCTTAACGCTTTCGGGTGGTGAACCCTTCATGCAGCCGGAAATGGCGACGGCGCTGCTGCAAGCCTGCCACGAGGCA
GGCATTCATACTGCGGTAGAAACCTGTCTGCATGTGCCGTGGAAATATATCGCCCCTTCTCTGCCCTATATCGATCTGTT
TCTTGCCGATTTAAAACACGTTGCCGACGCGCCGTTTAAACAGTGGACCGACGGTAACGCCGCCAGGGTGCTGGATAACC
TGAAAAAACTCGCCGCAGCGGGCAAAAAAATCATTATCCGCGTGCCGCTGATTCAGGGCTTTAATGCCGACGAAACCTCT
GTAAAAGCCATTACCGATTTTGCCGCCGACGAGCTGCACGTTGGCGAAATTCATTTTCTGCCCTACCACACGCTGGGCAT
CAACAAATATCACTTACTTAATCTGCCCTATGACGCCCCGGAAAAACCGCTTGATGCGCCAGAGCTGCTCGATTTTGCCC
AGCAGTATGCCTGCCAGAAAGGGTTAACCGCGACCTTACGAGGATAA

Upstream 100 bases:

>100_bases
CATCCTCAAAATGTACTTTTTGTCTGAATGTAGACTATCACGGGAAAACTCTCTGAACAGTTGACGCACATCAATCTAAC
TTTCATTCGAAAGTATTTTA

Downstream 100 bases:

>100_bases
CAACCATGACCACACTGAAACTGGACACGCTCAGCGACCGCATTAAAGCGCACAAAAATGCGCTGGTGCATATTGTGAAG
CCGCCAGTCTGTACCGAGCG

Product: putative pyruvate formate-lyase 2 activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3 [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPEVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Sequences:

>Translated_308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPEVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG
>Mature_308_residues
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLCLEGCELCAKAAPEVIERALN
GLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEA
GIHTAVETCLHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQKGLTATLRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=98.9966555183946, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1790389, Length=308, Percent_Identity=35.7142857142857, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1787130, Length=171, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790839, Length=236, Percent_Identity=25.4237288135593, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 34130; Mature: 34130

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLC
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHH
LEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMA
HHHHHHHHHHHHHHHHHHHHHHEEEHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETCLHVPWKYIAPS
HHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCC
LPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
CCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHH
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQK
HHHHHHHHCCCEEECEEEEECEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATLRG
CCEEECCC
>Mature Secondary Structure
MFERNREATMIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDLLYDARLC
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHH
LEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGEVKSVEEIMA
HHHHHHHHHHHHHHHHHHHHHHEEEHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETCLHVPWKYIAPS
HHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCC
LPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIRVPLIQGFNADETS
CCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHH
VKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLDFAQQYACQK
HHHHHHHHCCCEEECEEEEECEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GLTATLRG
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]