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The map label for this gene is prfH

Identifier: 30061853

GI number: 30061853

Start: 304523

End: 304978

Strand: Direct

Name: prfH

Synonym: S0305

Alternate gene names: 30061853

Gene position: 304523-304978 (Clockwise)

Preceding gene: 30061852

Following gene: 30061855

Centisome position: 6.62

GC content: 56.36

Gene sequence:

>456_bases
GTGAAAAAGGCGCTGGACAGGCTAATTAAAGAAGCTGCCCGACAAGACGTCGCGGTAACGGTGCTGGAAACAGAAACAGG
CCGCTACTCTGACACGCTGCGTTCGGCGCTGATTTCTCTGGATGGCGACAACGCATGGGCGTTAAGCGAAAGCTGGTGTG
GCACTATTCAGTGGATTTGCCCGAGCCCGTATCGCCCACATCACGGGCGTAAAAACTGGTTTCTGGGCATTGGGCGTTTT
ACCGCTGATGAGCAGGAGCAATCCGATGCGATCCGGTATGAGACGCTGCGTTCGTCGGGGCCGGGCGGTCAACATGTCAA
TAAAACCGACTCGGCGGTACGCGCCACGCATTTGGCATCCGGTATTAGCGTGAAGGTTCAGTCAGAGCGCAGCCAGCATG
CTAACAAACGGCTGGCGCGATTGCTGATTGCCTGGAAGCTGGATCAGCAGCAATAG

Upstream 100 bases:

>100_bases
GGCTGCGTCCGGTGCTAACGCTCAAAAACAGTGGAGGGAAAAAAGGATGATCTTGCTATAACTCTCCTCAGCGCAGGGGC
CGGAAGAATGTTGTCTCGCA

Downstream 100 bases:

>100_bases
GAAAATAGCGCGGTGCTGAAATCACAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGAACGTTTAC
AGGGATGGCTTTTATCGAAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRF
TADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLDQQQ

Sequences:

>Translated_151_residues
MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRF
TADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLDQQQ
>Mature_151_residues
MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRF
TADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWKLDQQQ

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=60, Percent_Identity=51.6666666666667, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1787462, Length=57, Percent_Identity=56.140350877193, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI2367172, Length=120, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17542784, Length=123, Percent_Identity=34.9593495934959, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321295, Length=57, Percent_Identity=57.8947368421053, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921226, Length=61, Percent_Identity=55.7377049180328, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 16956; Mature: 16956

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWIC
CHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEC
PSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLAS
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
GISVKVQSERSQHANKRLARLLIAWKLDQQQ
CCEEEEECHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDNAWALSESWCGTIQWIC
CHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEC
PSPYRPHHGRKNWFLGIGRFTADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLAS
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
GISVKVQSERSQHANKRLARLLIAWKLDQQQ
CCEEEEECHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]