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The map label for this gene is yjbJ [H]

Identifier: 30061800

GI number: 30061800

Start: 252970

End: 254700

Strand: Direct

Name: yjbJ [H]

Synonym: S0248

Alternate gene names: 30061800

Gene position: 252970-254700 (Clockwise)

Preceding gene: 30061799

Following gene: 30061801

Centisome position: 5.5

GC content: 50.78

Gene sequence:

>1731_bases
ATGGCTAATTACCGTGATTTGTTAGAGCAGGCTGGCGCACGTTACGGTGTGCCAGAAGGGTTGATGACTGCACTGGGTGC
CAAGGAGTCTTCTTACAACCCTGCCGCAGTAAGCTCCGCCGGGGCTGTAGGATTGACTCAGGTCATGCCTGGGACATGGC
GTGATATGGGTTATACCGATGAGCAAATGCAAAACCCCGAATATCAGGCTGACGCTGGCGCGCGCTATCTGGCAAAGATG
TACCAGCAGTTTGGTAACTGGCGTGACTCTCTTCAGGCTTATCACGACGGTCCCGGCAACGTTATGAAGGCAAAGCGTGG
TGAATATACGCCAGGACCTGAAGGCCGCGGTTACGTTGATGATCGCTTTGCTCAATGGGCTGGTGACCCGGTGACAGACT
CAACAGTCGAACAGCGCGCCACCTCTGCAAAGGTACATCCTCAGCAAGACCCTAACAACCCGTTTGCACAACTGGAAGTA
CAGTCATCCGAACAAGTATCGGCATCAGGTGTGCAGTCAGACCCAAATAATCCATTTGCTCAGATTGAGCAGCAGGCAGC
CAGTCAGCAGCCACCTCAACCCGTAAGTTCTGTCGCACCGAAACCTGTTCAGCAACAAACAGTTAATCAGGCCAATAATG
CACCAACACGGGAAGAACCATCATTGATGCAACAAGCTGGCGATTGGCTCACTGGTGGTCAAAGTGCAGGGCAAATTGCA
GAACAGGCTGGTCGTGGTCTGGTAAACATACCATTTGACGTATTGCAGGGTGGCGCAAGTCTGATTAATGCAATCAGTCA
GGGGCTTGGGGGGCCAAAAGTATTGGATGATGTTTATCGTCCAGTAGACAGACCGACAGACCCCTACGCGCAAGCTGGAG
AGTCAATAGGCGGTTATCTTGTTCCTGGAGCAGGTGTAGCTGGAAACATGGTCATTGGTTCTCTCGCTGACGCGGCGAAT
CAACGGGGTGATTTTGCCGAAAATGCCGCTATTAATGCCGGACTTAGCATTGCTACGCATGGCCTGATAAATGGCGTTAC
CCGTGGTGTTCGTGGTGCATCAAATATAATTAGTGGCAATAAAACATCTGCACAGAGAGCGACCACTGCGCCAACAGAAA
CATCACCATTCTCCGGTGATGCCGCTGCAGCAACAAATCCTGCGGTTCATGCCGCAGAGGCAAGAGTAGCACAAGGTGTA
CCAATGACGCCTGCGACGAAGAACCCAGAGGAAGTCGTTCGCACAGTAGCAGCACAAAAAAGGCCAAATCTCGCTTCATC
GCTTGATGAACTAGATATCAATCCTCAGGCTGAAGTTCTGGAGTCTGCTGAAAGGCTTAATGTTGATTCATTACTCCCTT
CACACTTTTCCGGGAACGAGCAATACAAGGCAGTTGAGCAAGCAATCAAGTCCCGTGCGGGTTCTGCTCTACAGGTGCAG
GAAAATGAAGCAATCAGGCAGCTAGCACAGGGCGCGGGTGAGATAATTGATCGCGTTTCCGGTGCAAAAGATGCTCTTGG
TATGAGCGACAAGTTTATTGATACGGTCAATGGAAGAATGTCTGCGCTGATGAAACGAAGCGACCAGCTTTATCGCAATG
TTGAAAAGGCGATGCCTGCAGGTGCAAAAATTGATGCGCCATCAACAAGGTCAATGCTCAAACAGGTGGCAGAAGATCTT
GGCGGGATGAAAAACCTTGACCCTATTGAAAAGAGAGTTGAGGTAGCCTGA

Upstream 100 bases:

>100_bases
GGAAGCGCCTCAGGCTTCTATAGATTACCTGAAGCAGCACCCCGAGCTGTCAATCGACTTCATTAATCGCTACGGATATC
TTCCATCTTTGGGGCAGTAA

Downstream 100 bases:

>100_bases
GTTTAACGGACACTCCTTCCTGAAATAGAATGGCATCAGAAGGAGCTAATAATGAGCAGAAAAACCCAACGTGAACCGCC
CCGGGAATCCTGGAGACTAA

Product: putative DNA transfer protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVGLTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKM
YQQFGNWRDSLQAYHDGPGNVMKAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTREEPSLMQQAGDWLTGGQSAGQIA
EQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAAN
QRGDFAENAAINAGLSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAAEARVAQGV
PMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQYKAVEQAIKSRAGSALQVQ
ENEAIRQLAQGAGEIIDRVSGAKDALGMSDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDL
GGMKNLDPIEKRVEVA

Sequences:

>Translated_576_residues
MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVGLTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKM
YQQFGNWRDSLQAYHDGPGNVMKAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTREEPSLMQQAGDWLTGGQSAGQIA
EQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAAN
QRGDFAENAAINAGLSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAAEARVAQGV
PMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQYKAVEQAIKSRAGSALQVQ
ENEAIRQLAQGAGEIIDRVSGAKDALGMSDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDL
GGMKNLDPIEKRVEVA
>Mature_575_residues
ANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVGLTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMY
QQFGNWRDSLQAYHDGPGNVMKAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEVQ
SSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTREEPSLMQQAGDWLTGGQSAGQIAE
QAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQ
RGDFAENAAINAGLSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAAEARVAQGVP
MTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQYKAVEQAIKSRAGSALQVQE
NEAIRQLAQGAGEIIDRVSGAKDALGMSDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLG
GMKNLDPIEKRVEVA

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=142, Percent_Identity=35.2112676056338, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 60803; Mature: 60672

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVGLTQVMPGTWRDMGYTD
CCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHCCCCCCCCCCCH
EQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVMKAKRGEYTPGPEGRGYVD
HHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCHH
DRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEVQSSEQVSASGVQSDPNNPFA
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCHHH
QIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTREEPSLMQQAGDWLTGGQSAGQIA
HHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHH
EQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGESIGGYL
HHHCCCEEECCHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEE
VPGAGVAGNMVIGSLADAANQRGDFAENAAINAGLSIATHGLINGVTRGVRGASNIISGN
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KTSAQRATTAPTETSPFSGDAAAATNPAVHAAEARVAQGVPMTPATKNPEEVVRTVAAQK
CCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
RPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQYKAVEQAIKSRAGSALQVQ
CCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEH
ENEAIRQLAQGAGEIIDRVSGAKDALGMSDKFIDTVNGRMSALMKRSDQLYRNVEKAMPA
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAKIDAPSTRSMLKQVAEDLGGMKNLDPIEKRVEVA
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
ANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVGLTQVMPGTWRDMGYTD
CCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHCCCCCCCCCCCH
EQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVMKAKRGEYTPGPEGRGYVD
HHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCHH
DRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEVQSSEQVSASGVQSDPNNPFA
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCHHH
QIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTREEPSLMQQAGDWLTGGQSAGQIA
HHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHH
EQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGESIGGYL
HHHCCCEEECCHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEE
VPGAGVAGNMVIGSLADAANQRGDFAENAAINAGLSIATHGLINGVTRGVRGASNIISGN
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KTSAQRATTAPTETSPFSGDAAAATNPAVHAAEARVAQGVPMTPATKNPEEVVRTVAAQK
CCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
RPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQYKAVEQAIKSRAGSALQVQ
CCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEH
ENEAIRQLAQGAGEIIDRVSGAKDALGMSDKFIDTVNGRMSALMKRSDQLYRNVEKAMPA
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GAKIDAPSTRSMLKQVAEDLGGMKNLDPIEKRVEVA
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]