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The map label for this gene is mraW

Identifier: 30061649

GI number: 30061649

Start: 88199

End: 89140

Strand: Direct

Name: mraW

Synonym: S0081

Alternate gene names: 30061649

Gene position: 88199-89140 (Clockwise)

Preceding gene: 30061648

Following gene: 30061650

Centisome position: 1.92

GC content: 55.2

Gene sequence:

>942_bases
ATGATGGAAAACTATAAACATACTACGGTGCTGCTGGATGAAGCCGTTAATGGCCTCAATATCCGTCCTGATGGCATCTA
CATTGATGGGACTTTTGGTCGCGGTGGTCACTCACGTCTGATCCTCTCGCAGCTTGGCGAAGAGGGGCGTTTGCTGGCGA
TCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCCACGGACCTTTCTCC
GCGCTGGGCGAATACGTTGCCGAGCGCGATCTTATTGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACC
GCAACTTGATGATGCTGAACGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGC
AGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGGTGAAGAGCATTTT
GCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGACCCGCACCAAAGAACTGGCGGAAGTCGT
GGCTGCTGCAACGCCGGTGAAAGATAAGTTTAAACATCCCGCGACCCGTACCTTCCAGGCGGTGCGCATTTGGGTAAACA
GTGAACTGGAGGAGATAGAGCAGGCGCTAAAAAGCTCGCTCAACGTGCTGGCCCCGGGTGGGCGGCTTTCGATCATCAGC
TTCCACTCGCTGGAAGACCGTATTGTGAAACGTTTTATGCGTGAAAACAGCCGCGGTCCGCAAGTTCCGGCAGGGTTACC
GATGACTGAAGAGCAGCTCAAAAAACTGGGTGGCCGTCAGCTGCGAGCACTAGGCAAGTTAATGCCGGGCGAAGAAGAGG
TGGCTGAGAACCCTCGTGCCCGTAGTTCAGTTCTGCGTATTGCAGAGAGGACGAACGCATGA

Upstream 100 bases:

>100_bases
GATGAAACAACCTGGCATCAACAGGTCAAGGAAGATATCGACGCAGAGCAGTTGGCTACCGGAGACTTATCGGAGCGACT
GCAGGACTTGTCTCTATAAA

Downstream 100 bases:

>100_bases
TCAGCAGAGTGACAGAAGCTCTAAGCAAAGTTAAAGGATCGATGGGAAGCCACGAGCGCCATGCATTGCCTGGTGTTATC
GGTGACGATCTTTTGCGATT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFS
ALGEYVAERDLIGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLNVLAPGGRLSIIS
FHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKLGGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA

Sequences:

>Translated_313_residues
MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFS
ALGEYVAERDLIGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLNVLAPGGRLSIIS
FHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKLGGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA
>Mature_313_residues
MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFS
ALGEYVAERDLIGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLNVLAPGGRLSIIS
FHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKLGGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=39.7626112759644, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=42.1875, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=99.6805111821086, Blast_Score=632, Evalue=0.0,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=40.8408408408408, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=40.8408408408408, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34859; Mature: 34859

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQA
CCCCCCHHHEEHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCE
IAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDLGVSSPQLDDAERGFSFMR
EEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCHHHHC
DGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHFAKRIARAIVERNREQPMTRT
CCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHH
KELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLNVLAPGGRLSIIS
HHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCEEEEE
FHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKLGGRQLRALGKLMPGEEEVAENPRA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCHHHHHCCCHH
RSSVLRIAERTNA
HHHHHHHHHHCCC
>Mature Secondary Structure
MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQA
CCCCCCHHHEEHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCE
IAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDLGVSSPQLDDAERGFSFMR
EEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCHHHHC
DGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHFAKRIARAIVERNREQPMTRT
CCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHH
KELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLNVLAPGGRLSIIS
HHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCEEEEE
FHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKLGGRQLRALGKLMPGEEEVAENPRA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCHHHHHCCCHH
RSSVLRIAERTNA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA