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The map label for this gene is gef

Identifier: 30061586

GI number: 30061586

Start: 15401

End: 15610

Strand: Reverse

Name: gef

Synonym: S0017

Alternate gene names: 30061586

Gene position: 15610-15401 (Counterclockwise)

Preceding gene: 30061589

Following gene: 30061583

Centisome position: 0.34

GC content: 51.43

Gene sequence:

>210_bases
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Upstream 100 bases:

>100_bases
TAGCGGCGGGTGCTTGAGGCTGTCTGTCTCAGGCATTAGCTGAACGGCAGATAGAGAAAAGCCCCGAGTGATATTTTACC
ATCAACCCGAGGCCTCCTAT

Downstream 100 bases:

>100_bases
GAGCAACGGCGGGGAGTGATCCCCGCCACTTTACAGGTGCTCGCATATCTTCAACGCACCCTATTTTTACCCAGGCCTGC
CCACGGGCAGGCTTTTGGGG

Product: membrane toxin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 69

Protein sequence:

>69_residues
MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVFTAYESE

Sequences:

>Translated_69_residues
MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVFTAYESE
>Mature_69_residues
MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVFTAYESE

Specific function: Overlapping regulatory peptide whose translation enables hokC expression (Probable)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hok/gef family

Homologues:

Organism=Escherichia coli, GI1786200, Length=69, Percent_Identity=100, Blast_Score=139, Evalue=4e-35,
Organism=Escherichia coli, GI48994874, Length=50, Percent_Identity=100, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI1787845, Length=50, Percent_Identity=82, Blast_Score=86, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOKC_ECOLI (P33236)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_000681.1
- RefSeq:   NP_414559.1
- ProteinModelPortal:   P33236
- EnsemblBacteria:   EBESCT00000004815
- EnsemblBacteria:   EBESCT00000018269
- GeneID:   944756
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5879
- KEGG:   eco:b0018
- EchoBASE:   EB1999
- EcoGene:   EG12074
- GeneTree:   EBGT00050000009513
- HOGENOM:   HBG473224
- OMA:   HKVMIVA
- ProtClustDB:   CLSK879539
- BioCyc:   EcoCyc:EG10373-MONOMER
- Genevestigator:   P33236
- InterPro:   IPR000021
- InterPro:   IPR018084
- PRINTS:   PR00281
- ProDom:   PD005979

Pfam domain/function: PF01848 HOK_GEF

EC number: NA

Molecular weight: Translated: 7742; Mature: 7742

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00556 HOK_GEF

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xfe2f4ec)-;

Cys/Met content:

4.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
AVFTAYESE
EEEEEECCH
>Mature Secondary Structure
MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
AVFTAYESE
EEEEEECCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1943701; 9278503; 10361310