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The map label for this gene is ywaE

Identifier: 30024073

GI number: 30024073

Start: 2202406

End: 2202885

Strand: Reverse

Name: ywaE

Synonym: L185851

Alternate gene names: 30024073

Gene position: 2202885-2202406 (Counterclockwise)

Preceding gene: 15674117

Following gene: 15674115

Centisome position: 93.12

GC content: 35.21

Gene sequence:

>480_bases
ATGAAGATTAAATTAGTAGTTGTTGGAAAACTTAAAGAAAAGTATTTAAAAGATGGAATTGCTGAATATGTTAAACGAAT
GGGAACAATGCTGCCACTTGAAATTATTGAGCTAGCAGATGAAAAAATTCCAGATAATGCTTCCGAAAAAGAAGCAGAGG
CATTGAAAAAACGTGAAGGTGAGAAAATTTTATCACGGATTCAAGCAGGAGATCAGTTAGCGATTTTAGCCATTCAAGGA
AAATTAATGTCATCAGAGGAAGTGGCTGATTTTGTGAAGAAAGCTGAAGTTTACGGAACAGGAAATCTTGTTTTTGTAAT
TGGAGGTTCTTTAGGACTTTCAAAGGAAGTCTATCAACGAAGTGATTTGCAAATTTCTTTTGGGCGAATGACACTTCCTC
ATCAACTGATGCGTCTGGTTTTAGTAGAACAAATTTATCGGGCACAAATGATTAATCGAGGTTCGGCTTATCATAAGTAA

Upstream 100 bases:

>100_bases
ACCATTATCCAAGAAAAATGTTCAACTATAAGAGTTCATATGAGATGAGCTTGGCTGGCTAACTTGGACTTGCAATTTGC
GTTTAGAGAGAAAAATAAAA

Downstream 100 bases:

>100_bases
AAATTTGAAAGGAATGAAATGATTAATATTATAGAAGAGTTTCGCCAATATAAGAATGAAGAAAATGCTGAAAAACAAGC
AGCATATTTGCGTCACCAGT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREGEKILSRIQAGDQLAILAIQG
KLMSSEEVADFVKKAEVYGTGNLVFVIGGSLGLSKEVYQRSDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSAYHK

Sequences:

>Translated_159_residues
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREGEKILSRIQAGDQLAILAIQG
KLMSSEEVADFVKKAEVYGTGNLVFVIGGSLGLSKEVYQRSDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSAYHK
>Mature_159_residues
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREGEKILSRIQAGDQLAILAIQG
KLMSSEEVADFVKKAEVYGTGNLVFVIGGSLGLSKEVYQRSDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSAYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=160, Percent_Identity=31.25, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_LACLA (Q9CDS7)

Other databases:

- EMBL:   AE005176
- PIR:   F86891
- RefSeq:   NP_268291.2
- ProteinModelPortal:   Q9CDS7
- SMR:   Q9CDS7
- GeneID:   1115811
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L185851
- NMPDR:   fig|272623.1.peg.2189
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   LLAC272623:L185851-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17838; Mature: 17838

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREG
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EKILSRIQAGDQLAILAIQGKLMSSEEVADFVKKAEVYGTGNLVFVIGGSLGLSKEVYQR
HHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHC
SDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSAYHK
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIKLVVVGKLKEKYLKDGIAEYVKRMGTMLPLEIIELADEKIPDNASEKEAEALKKREG
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EKILSRIQAGDQLAILAIQGKLMSSEEVADFVKKAEVYGTGNLVFVIGGSLGLSKEVYQR
HHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHC
SDLQISFGRMTLPHQLMRLVLVEQIYRAQMINRGSAYHK
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471