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The map label for this gene is pth

Identifier: 29349996

GI number: 29349996

Start: 6010959

End: 6011528

Strand: Reverse

Name: pth

Synonym: BT_4588

Alternate gene names: 29349996

Gene position: 6011528-6010959 (Counterclockwise)

Preceding gene: 29349997

Following gene: 29349995

Centisome position: 96.03

GC content: 43.16

Gene sequence:

>570_bases
ATGATTAAATATCTAATTGTCGGACTAGGAAACATTGGACCCGAATATCATGAAACCCGGCATAATATTGGTTTCATGAC
AGTAGAGGCATTGGCAAGAATCAACAATGCTCCTCCCTTTATTGACGGACGCTACGGATTTACCACTAGTTTTTCGATCA
AAGGACGCCAGTTAATCCTGCTCAAACCGTCAACTTTCATGAACCTGAGCGGACTGGCCGTTCGCTACTGGATGCAAAAA
GAAAACATCCCTTTGGAAAATGTGTTAATAGTAGTAGACGATCTGGCTCTCCCCTTCGGCACTCTGCGTCTGAAAGGAAA
AGGAAGTGATGCCGGACATAATGGTCTGAAGCACATAGCAGCCATTTTAGGAACTCAGAACTATGCCCGTTTACGCTTTG
GTATCGGAAATGACTTCCCCAAAGGCGGACAGATTGATTATGTACTCGGACACTTTACTGACGAAGATCGCAAGACAATG
GATGAAAGACTGGAAACAGCCGGAGAAATCATTAAAAGTTTCTGTCTGGCAGGTATTGATATTACCATGAACCAGTTCAA
TAAGAAATAG

Upstream 100 bases:

>100_bases
GCAGATTAATGCAGATTAAAGCAGATGTTCAGTCTGCATTAGTTTGCGTTAATTTGCGTTTCTCTTTTTATATTCACGAA
CTGTTTTTTACGAAAAAACA

Downstream 100 bases:

>100_bases
GAGTATGTACAATTGACTATTTCACAGCGTATATATGCAAACGGTCATTAAGTACTCCACCATGACACATGGTACACTGT
TAATTAATAAATTATCAATA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MIKYLIVGLGNIGPEYHETRHNIGFMTVEALARINNAPPFIDGRYGFTTSFSIKGRQLILLKPSTFMNLSGLAVRYWMQK
ENIPLENVLIVVDDLALPFGTLRLKGKGSDAGHNGLKHIAAILGTQNYARLRFGIGNDFPKGGQIDYVLGHFTDEDRKTM
DERLETAGEIIKSFCLAGIDITMNQFNKK

Sequences:

>Translated_189_residues
MIKYLIVGLGNIGPEYHETRHNIGFMTVEALARINNAPPFIDGRYGFTTSFSIKGRQLILLKPSTFMNLSGLAVRYWMQK
ENIPLENVLIVVDDLALPFGTLRLKGKGSDAGHNGLKHIAAILGTQNYARLRFGIGNDFPKGGQIDYVLGHFTDEDRKTM
DERLETAGEIIKSFCLAGIDITMNQFNKK
>Mature_189_residues
MIKYLIVGLGNIGPEYHETRHNIGFMTVEALARINNAPPFIDGRYGFTTSFSIKGRQLILLKPSTFMNLSGLAVRYWMQK
ENIPLENVLIVVDDLALPFGTLRLKGKGSDAGHNGLKHIAAILGTQNYARLRFGIGNDFPKGGQIDYVLGHFTDEDRKTM
DERLETAGEIIKSFCLAGIDITMNQFNKK

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=172, Percent_Identity=30.8139534883721, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1787455, Length=189, Percent_Identity=37.037037037037, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_BACTN (Q89YZ2)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_813499.1
- ProteinModelPortal:   Q89YZ2
- SMR:   Q89YZ2
- GeneID:   1072118
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4588
- NMPDR:   fig|226186.1.peg.4586
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   BTHE226186:BT_4588-MONOMER
- BRENDA:   3.1.1.29
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21090; Mature: 21090

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKYLIVGLGNIGPEYHETRHNIGFMTVEALARINNAPPFIDGRYGFTTSFSIKGRQLIL
CEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCEEEE
LKPSTFMNLSGLAVRYWMQKENIPLENVLIVVDDLALPFGTLRLKGKGSDAGHNGLKHIA
ECCCCCCCCCCCEEEEEECCCCCCHHHEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHH
AILGTQNYARLRFGIGNDFPKGGQIDYVLGHFTDEDRKTMDERLETAGEIIKSFCLAGID
HHHCCCCCEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
ITMNQFNKK
EEHHCCCCC
>Mature Secondary Structure
MIKYLIVGLGNIGPEYHETRHNIGFMTVEALARINNAPPFIDGRYGFTTSFSIKGRQLIL
CEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCEEEE
LKPSTFMNLSGLAVRYWMQKENIPLENVLIVVDDLALPFGTLRLKGKGSDAGHNGLKHIA
ECCCCCCCCCCCEEEEEECCCCCCHHHEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHH
AILGTQNYARLRFGIGNDFPKGGQIDYVLGHFTDEDRKTMDERLETAGEIIKSFCLAGID
HHHCCCCCEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
ITMNQFNKK
EEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928