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The map label for this gene is apbE [H]

Identifier: 29349968

GI number: 29349968

Start: 5981615

End: 5982631

Strand: Reverse

Name: apbE [H]

Synonym: BT_4560

Alternate gene names: 29349968

Gene position: 5982631-5981615 (Counterclockwise)

Preceding gene: 29349969

Following gene: 29349965

Centisome position: 95.56

GC content: 43.07

Gene sequence:

>1017_bases
ATGAAAACAAAAAAGAGTTTCCTTTGGCTGGCCTTCCTCATCTTGGCAACGATCTGGATATTAGCCAGACGTAACCAAAA
AGCAGAGTTTAATACCGCCAGTGGTTTTGTGTTTGGGACAGTATACAAGATCGCGTATCAACATGACGCTGATCTGAAAC
CGGAGATTGAAGCGGAATTAAAACGTTTCGACCAGTCTCTCTCACCGTTCAATGACAGTTCGGTCATTAGTCGCGTCAAT
CGCAACGAAGAGTTGGTGACAGACAGCTTTTTCCAAACTTGCTTCAACCGTTCTATGGAAATCTCCCGTGAAACAAAAGG
AGCTTTTGACATAACGATAGCCCCTCTTGCCAATGCCTGGGGATTTGGCTTTAAGAAAGGCGCCTTCCCTGATTCATTGA
TGATAGACAGCCTGCTACAGATTACCGGTTACGAAAAAGTGAAGCTGGAAAACGGAAAGGTAGTCAAGCAAGATCCGCGT
GTAATGTTAAGTTGCAGTGCGGTTGCAAAAGGCTATTCGGTAGATGTAATAGCCCGTCTACTCGATCGGAAAGGAATCAA
AAACTATATGGTAGATATAGGTGGTGAAGTAGTAGTAAAAGGTAAAAATGCGACAGGAGATCTGTGGCGTATCGGAATAA
ACAAGCCGTACGACGACTCATTAGCTGTTAAGCAAGATATACAAGTAGTACTCAACCTGACAGATTTAGGTATGGCTACT
TCGGGAAACTACCGCAACTATTATTATAAGGATGGCAAAAAGTATGCTCATACCATCGACCCCAGAACAGGTTATCCGGT
ACAGCACAGTATCCTATCTTCTACTGTGATTGCCGAAGACTGTATGACGGCTGATGCTTTGGCTACCTCTTTCATGGTAA
TGGAACTGGAAGAAGCCGAAAAATTCTGTAAAGCAAATCCTATGATCGATGCCTACTTTATATATAGCGGCGAGAATGGA
GAGTTTAAGACTTACTATACGGATGGTATGAAACGATATATGCCTGATGCCAAATAG

Upstream 100 bases:

>100_bases
CGGCATCATTCTAGTCGCTATCGGATTAAAAATATTGATAGAACATCTATTCTTACAATAATAAGCATTGGTTCCTCTAA
TAAAAAAAACAGAAAAACGT

Downstream 100 bases:

>100_bases
AAAAATGCTTTACAAATAAGCATATAAACGGAGGCTCCATTTCATACGAATAGAGCCTCCGTTTATATAAAACAGCACCT
CTGTTGAAGCCAAACGAAGC

Product: thiamine biosynthesis lipoprotein apbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKTKKSFLWLAFLILATIWILARRNQKAEFNTASGFVFGTVYKIAYQHDADLKPEIEAELKRFDQSLSPFNDSSVISRVN
RNEELVTDSFFQTCFNRSMEISRETKGAFDITIAPLANAWGFGFKKGAFPDSLMIDSLLQITGYEKVKLENGKVVKQDPR
VMLSCSAVAKGYSVDVIARLLDRKGIKNYMVDIGGEVVVKGKNATGDLWRIGINKPYDDSLAVKQDIQVVLNLTDLGMAT
SGNYRNYYYKDGKKYAHTIDPRTGYPVQHSILSSTVIAEDCMTADALATSFMVMELEEAEKFCKANPMIDAYFIYSGENG
EFKTYYTDGMKRYMPDAK

Sequences:

>Translated_338_residues
MKTKKSFLWLAFLILATIWILARRNQKAEFNTASGFVFGTVYKIAYQHDADLKPEIEAELKRFDQSLSPFNDSSVISRVN
RNEELVTDSFFQTCFNRSMEISRETKGAFDITIAPLANAWGFGFKKGAFPDSLMIDSLLQITGYEKVKLENGKVVKQDPR
VMLSCSAVAKGYSVDVIARLLDRKGIKNYMVDIGGEVVVKGKNATGDLWRIGINKPYDDSLAVKQDIQVVLNLTDLGMAT
SGNYRNYYYKDGKKYAHTIDPRTGYPVQHSILSSTVIAEDCMTADALATSFMVMELEEAEKFCKANPMIDAYFIYSGENG
EFKTYYTDGMKRYMPDAK
>Mature_338_residues
MKTKKSFLWLAFLILATIWILARRNQKAEFNTASGFVFGTVYKIAYQHDADLKPEIEAELKRFDQSLSPFNDSSVISRVN
RNEELVTDSFFQTCFNRSMEISRETKGAFDITIAPLANAWGFGFKKGAFPDSLMIDSLLQITGYEKVKLENGKVVKQDPR
VMLSCSAVAKGYSVDVIARLLDRKGIKNYMVDIGGEVVVKGKNATGDLWRIGINKPYDDSLAVKQDIQVVLNLTDLGMAT
SGNYRNYYYKDGKKYAHTIDPRTGYPVQHSILSSTVIAEDCMTADALATSFMVMELEEAEKFCKANPMIDAYFIYSGENG
EFKTYYTDGMKRYMPDAK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=325, Percent_Identity=32.9230769230769, Blast_Score=185, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38106; Mature: 38106

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKKSFLWLAFLILATIWILARRNQKAEFNTASGFVFGTVYKIAYQHDADLKPEIEAEL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEEECCCCCCCCHHHHHH
KRFDQSLSPFNDSSVISRVNRNEELVTDSFFQTCFNRSMEISRETKGAFDITIAPLANAW
HHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
GFGFKKGAFPDSLMIDSLLQITGYEKVKLENGKVVKQDPRVMLSCSAVAKGYSVDVIARL
CCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCCCEEEEEHHHHCCCCHHHHHHH
LDRKGIKNYMVDIGGEVVVKGKNATGDLWRIGINKPYDDSLAVKQDIQVVLNLTDLGMAT
HHHCCHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHEEEEHHHCCEEC
SGNYRNYYYKDGKKYAHTIDPRTGYPVQHSILSSTVIAEDCMTADALATSFMVMELEEAE
CCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHH
KFCKANPMIDAYFIYSGENGEFKTYYTDGMKRYMPDAK
HHHHCCCCEEEEEEEECCCCCEEEEEECCHHHHCCCCC
>Mature Secondary Structure
MKTKKSFLWLAFLILATIWILARRNQKAEFNTASGFVFGTVYKIAYQHDADLKPEIEAEL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEEECCCCCCCCHHHHHH
KRFDQSLSPFNDSSVISRVNRNEELVTDSFFQTCFNRSMEISRETKGAFDITIAPLANAW
HHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCC
GFGFKKGAFPDSLMIDSLLQITGYEKVKLENGKVVKQDPRVMLSCSAVAKGYSVDVIARL
CCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCCCEEEEEHHHHCCCCHHHHHHH
LDRKGIKNYMVDIGGEVVVKGKNATGDLWRIGINKPYDDSLAVKQDIQVVLNLTDLGMAT
HHHCCHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHEEEEHHHCCEEC
SGNYRNYYYKDGKKYAHTIDPRTGYPVQHSILSSTVIAEDCMTADALATSFMVMELEEAE
CCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHH
KFCKANPMIDAYFIYSGENGEFKTYYTDGMKRYMPDAK
HHHHCCCCEEEEEEEECCCCCEEEEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]