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The map label for this gene is phoB [H]

Identifier: 29349116

GI number: 29349116

Start: 4818649

End: 4820052

Strand: Reverse

Name: phoB [H]

Synonym: BT_3708

Alternate gene names: 29349116

Gene position: 4820052-4818649 (Counterclockwise)

Preceding gene: 29349117

Following gene: 29349115

Centisome position: 76.99

GC content: 46.58

Gene sequence:

>1404_bases
ATGAAAAAGTTGATTTACACACTGCTCTTTGTCCTTATTTCCGTTGCAGCCAACGGTCAGCAGGCAAAGTATGTATTCTA
TTTCATCGGAGACGGAATGGGAGTCAATCAGGTAAATGGTACAGAGATGTATCAGGCAGAACTGCAAAACGGGCGCATCG
GCGTTGAACCTTTATTGTTCACCCAATTTCCGGTAGCAACGATGGCAACCACTTTCTCCGCGACAAACTCTGTTACCGAC
TCTGCCGCTGCGGGTACTGCACTGGCTACCGGAAAGAAGACCTACAACAGTGCCATCTCTGTAGGAGAAGACAAAAACCC
GATTGAAACCGTAGCCGAAAAAGCCAAGAAGGCCGGAAAGAAAGTAGGTGTCACTACTTCCGTCAGCGTAGACCACGCCA
CTCCTGCCGCTTTCTACGCTCATCAGGCAGACCGTAATATGAATTATGAAATCGCTGTCGACCTGACAAAAGCAAACTTT
GACTTCTATGCAGGCGGTGGTTTTCTGAAACCGGACAAGACGTATGACAAGAAAGACGCTCCCAACATCTTCCCTATTTT
CGAAGAAGCAGGTTACACCGTAGCACGTGGCTACAGCGATTATAAAGCCAAAAGCAAGGATGCCGGCAAAATGATCCTGA
TACAGGAAGAAGGCAAAGATCCATCCTGCCTGCCTTATGCTATCGACCGTAAAAGCGACGACTTGACTCTCGCCCAAATC
ACAGAAAGCGCAATCGACTTCCTGACCAAAGGCAAGAACAAAGGATTCTTCTTAATGGTAGAAGGTGGAAAGATTGACTG
GGCTTGCCACGGTAATGACGCCGCTACCGTATTCAATGAAGTAAAGGACATGGATGACGCTATCAAAGTAGCTTACGAAT
TCTACAAGAAACATCCGAAAGAAACACTGATCGTTGTTACGGCCGACCACGAAACCGGTGGTATCGTACTAGGTACAGGC
AAATATGCGTTGAACCTGAAAGCATTGCAATATCAAAAACATTCTGCTGACGGATTGTCCAGACGTATCAGCGAACTAAG
AAAATCAAAAGGCAACAAAGTGACTTGGGAAGACATGAAAGAATTCCTGGGTGAAGAAATGGGCTTCTGGAAACAGTTCC
CTATTTCCTGGGAACAGGAAAAGAAGCTGCGTGACGAGTTCGAACAATCCTTTGTCAGAAATAAAGTGGTATTTGCCGAA
AGCATGTACTCTAAAAGCGAGCCGATGGCTGCACGCGCTAAAGAAGTAATGGACCAGATATCTATGGTAGGCTGGGTAAG
CGGCGGTCACTCTGCCGGTTATGTGCCGGTATTTGCTATCGGTGCCGGTTCTCAACTGTTCGGTGAGAAGATTGACAATA
CGGAAATTCCGAAACGGATAGCTAAAGCTGCCGGATATAAATAA

Upstream 100 bases:

>100_bases
TCATACAAATGACATAAAGAAACGGTGGATTTGCTTCGGCACTTCCACCGTTTTTTATTACCTTTGTCCATCACTAACCC
TAATATTAAGATACAAAAGA

Downstream 100 bases:

>100_bases
AGAATTGACTGCACGAATATGTAATCAAAACACTATTTATTTTCATTTAGGCACGGATTTCACGGATTAACACGGTTCTA
TTCAATTAAAGACCGTGAAA

Product: alkaline phosphatase III

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKKLIYTLLFVLISVAANGQQAKYVFYFIGDGMGVNQVNGTEMYQAELQNGRIGVEPLLFTQFPVATMATTFSATNSVTD
SAAAGTALATGKKTYNSAISVGEDKNPIETVAEKAKKAGKKVGVTTSVSVDHATPAAFYAHQADRNMNYEIAVDLTKANF
DFYAGGGFLKPDKTYDKKDAPNIFPIFEEAGYTVARGYSDYKAKSKDAGKMILIQEEGKDPSCLPYAIDRKSDDLTLAQI
TESAIDFLTKGKNKGFFLMVEGGKIDWACHGNDAATVFNEVKDMDDAIKVAYEFYKKHPKETLIVVTADHETGGIVLGTG
KYALNLKALQYQKHSADGLSRRISELRKSKGNKVTWEDMKEFLGEEMGFWKQFPISWEQEKKLRDEFEQSFVRNKVVFAE
SMYSKSEPMAARAKEVMDQISMVGWVSGGHSAGYVPVFAIGAGSQLFGEKIDNTEIPKRIAKAAGYK

Sequences:

>Translated_467_residues
MKKLIYTLLFVLISVAANGQQAKYVFYFIGDGMGVNQVNGTEMYQAELQNGRIGVEPLLFTQFPVATMATTFSATNSVTD
SAAAGTALATGKKTYNSAISVGEDKNPIETVAEKAKKAGKKVGVTTSVSVDHATPAAFYAHQADRNMNYEIAVDLTKANF
DFYAGGGFLKPDKTYDKKDAPNIFPIFEEAGYTVARGYSDYKAKSKDAGKMILIQEEGKDPSCLPYAIDRKSDDLTLAQI
TESAIDFLTKGKNKGFFLMVEGGKIDWACHGNDAATVFNEVKDMDDAIKVAYEFYKKHPKETLIVVTADHETGGIVLGTG
KYALNLKALQYQKHSADGLSRRISELRKSKGNKVTWEDMKEFLGEEMGFWKQFPISWEQEKKLRDEFEQSFVRNKVVFAE
SMYSKSEPMAARAKEVMDQISMVGWVSGGHSAGYVPVFAIGAGSQLFGEKIDNTEIPKRIAKAAGYK
>Mature_467_residues
MKKLIYTLLFVLISVAANGQQAKYVFYFIGDGMGVNQVNGTEMYQAELQNGRIGVEPLLFTQFPVATMATTFSATNSVTD
SAAAGTALATGKKTYNSAISVGEDKNPIETVAEKAKKAGKKVGVTTSVSVDHATPAAFYAHQADRNMNYEIAVDLTKANF
DFYAGGGFLKPDKTYDKKDAPNIFPIFEEAGYTVARGYSDYKAKSKDAGKMILIQEEGKDPSCLPYAIDRKSDDLTLAQI
TESAIDFLTKGKNKGFFLMVEGGKIDWACHGNDAATVFNEVKDMDDAIKVAYEFYKKHPKETLIVVTADHETGGIVLGTG
KYALNLKALQYQKHSADGLSRRISELRKSKGNKVTWEDMKEFLGEEMGFWKQFPISWEQEKKLRDEFEQSFVRNKVVFAE
SMYSKSEPMAARAKEVMDQISMVGWVSGGHSAGYVPVFAIGAGSQLFGEKIDNTEIPKRIAKAAGYK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266292, Length=348, Percent_Identity=29.8850574712644, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI94721246, Length=346, Percent_Identity=30.0578034682081, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI157266296, Length=350, Percent_Identity=31.1428571428571, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI116734717, Length=354, Percent_Identity=27.9661016949153, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI294660770, Length=333, Percent_Identity=27.6276276276276, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI48994877, Length=344, Percent_Identity=28.1976744186047, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320689, Length=472, Percent_Identity=27.3305084745763, Blast_Score=119, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24651554, Length=409, Percent_Identity=28.1173594132029, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24651556, Length=396, Percent_Identity=27.5252525252525, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI18859923, Length=333, Percent_Identity=25.5255255255255, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355151, Length=492, Percent_Identity=27.0325203252033, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21358067, Length=346, Percent_Identity=29.1907514450867, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19921912, Length=348, Percent_Identity=29.0229885057471, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24657835, Length=477, Percent_Identity=24.7379454926625, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24657842, Length=372, Percent_Identity=26.6129032258064, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355981, Length=331, Percent_Identity=28.7009063444109, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355149, Length=343, Percent_Identity=25.9475218658892, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585213, Length=356, Percent_Identity=29.2134831460674, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI85725200, Length=345, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI85815833, Length=345, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24657827, Length=335, Percent_Identity=28.0597014925373, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 51400; Mature: 51400

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIYTLLFVLISVAANGQQAKYVFYFIGDGMGVNQVNGTEMYQAELQNGRIGVEPLLF
CHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCEEEE
TQFPVATMATTFSATNSVTDSAAAGTALATGKKTYNSAISVGEDKNPIETVAEKAKKAGK
ECCCHHHHHHHHHCCCCCCCHHHCCCHHHCCCHHHHHHEECCCCCCHHHHHHHHHHHCCC
KVGVTTSVSVDHATPAAFYAHQADRNMNYEIAVDLTKANFDFYAGGGFLKPDKTYDKKDA
CCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCCC
PNIFPIFEEAGYTVARGYSDYKAKSKDAGKMILIQEEGKDPSCLPYAIDRKSDDLTLAQI
CCEEEEHHHCCCEEECCCHHHCCCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHH
TESAIDFLTKGKNKGFFLMVEGGKIDWACHGNDAATVFNEVKDMDDAIKVAYEFYKKHPK
HHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETLIVVTADHETGGIVLGTGKYALNLKALQYQKHSADGLSRRISELRKSKGNKVTWEDMK
CEEEEEEECCCCCCEEEECCCEEEEEEHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHH
EFLGEEMGFWKQFPISWEQEKKLRDEFEQSFVRNKVVFAESMYSKSEPMAARAKEVMDQI
HHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SMVGWVSGGHSAGYVPVFAIGAGSQLFGEKIDNTEIPKRIAKAAGYK
HHHEEECCCCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLIYTLLFVLISVAANGQQAKYVFYFIGDGMGVNQVNGTEMYQAELQNGRIGVEPLLF
CHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCEEEE
TQFPVATMATTFSATNSVTDSAAAGTALATGKKTYNSAISVGEDKNPIETVAEKAKKAGK
ECCCHHHHHHHHHCCCCCCCHHHCCCHHHCCCHHHHHHEECCCCCCHHHHHHHHHHHCCC
KVGVTTSVSVDHATPAAFYAHQADRNMNYEIAVDLTKANFDFYAGGGFLKPDKTYDKKDA
CCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCCC
PNIFPIFEEAGYTVARGYSDYKAKSKDAGKMILIQEEGKDPSCLPYAIDRKSDDLTLAQI
CCEEEEHHHCCCEEECCCHHHCCCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHH
TESAIDFLTKGKNKGFFLMVEGGKIDWACHGNDAATVFNEVKDMDDAIKVAYEFYKKHPK
HHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETLIVVTADHETGGIVLGTGKYALNLKALQYQKHSADGLSRRISELRKSKGNKVTWEDMK
CEEEEEEECCCCCCEEEECCCEEEEEEHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHH
EFLGEEMGFWKQFPISWEQEKKLRDEFEQSFVRNKVVFAESMYSKSEPMAARAKEVMDQI
HHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SMVGWVSGGHSAGYVPVFAIGAGSQLFGEKIDNTEIPKRIAKAAGYK
HHHEEECCCCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]