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The map label for this gene is mraW [H]

Identifier: 29348864

GI number: 29348864

Start: 4451155

End: 4452069

Strand: Reverse

Name: mraW [H]

Synonym: BT_3455

Alternate gene names: 29348864

Gene position: 4452069-4451155 (Counterclockwise)

Preceding gene: 29348865

Following gene: 29348863

Centisome position: 71.12

GC content: 50.6

Gene sequence:

>915_bases
ATGGAGAAAGATGAGTTGACATACCACGTACCCGTACTGCTAAAAGAAAGCGTTGACGGAATGAATATTCATCCGGACGG
AACGTATGTAGACGTTACCTTCGGAGGAGCAGGGCATTCGCGCGAGATTCTTTCACGCCTTGGAGAAGGCGGGCGCCTGC
TGGGATTCGATCAGGACGAGGATGCCGAACGGAATATCGTCAATGATCCCCACTTCACCTTTGTACGCAGCAACTTCCGC
TATTTGCAGAATTTCCTGCGGTATCATGACATCGAACAGGTGGACGCAATCCTCGCCGACCTCGGCGTGTCCTCCCACCA
TTTCGACGACAGCGAGCGTGGCTTCTCATTCCGTTTTGACGGAGCGCTGGATATGCGCATGAACAAACGTGCAGGACTAA
CTGCCGCCGACATCGTCAACACATACGAAGAAGAACGGCTCGCAAACATCTTCTATCTGTACGGTGAACTGAAGAACAGC
CGCAAACTGGCATCCGCCATCGTAAAAGCCAGAAACGGACAGTCTATCCGGACTATCGGCGAGTTTCTGGAAATCATCAA
GCCGCTTTTCGGACGCGAACGGGAGAAGAAAGAACTGGCGAAAGTATTTCAGGCACTCCGCATTGAAGTGAATCAGGAAA
TGGAAGCACTGAAAGAAATGCTGGCAGCAGCCACCGAAGCGCTCAAACCGGGGGGGCGCCTTGTAGTGATCACGTATCAC
TCACTGGAAGACCGCATGGTGAAGAATATAATGAAAACAGGAAACGTAGAAGGGAAAGCGGAAACCGACTTCTTTGGAAA
CCTGCAAACTCCGTTCCGGTTGGTCAACAATAAAGTGATTGTCCCCGATGAAGCGGAGATAGAAAGAAATCCACGGTCGA
GAAGTGCCAAATTACGAATCGCAGAGAAGAAATAA

Upstream 100 bases:

>100_bases
ATAGAGATTTGGGCCAAAGAACGGGCAGAACAACCGTTCATGTCCCCCGAAGAGTTCGGTGCAGCATTAGAAGAGATTAT
GAACGATGAAAATAGACAAG

Downstream 100 bases:

>100_bases
TCTCAGATATGGAAGAAGAAGCAGTAAACAAAAAAGCAGAAGAGGGCAAAAAGAAAAAACGCACCTCATTGAAAAGTATT
CTGGGAGGAGATATCCTCGC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MEKDELTYHVPVLLKESVDGMNIHPDGTYVDVTFGGAGHSREILSRLGEGGRLLGFDQDEDAERNIVNDPHFTFVRSNFR
YLQNFLRYHDIEQVDAILADLGVSSHHFDDSERGFSFRFDGALDMRMNKRAGLTAADIVNTYEEERLANIFYLYGELKNS
RKLASAIVKARNGQSIRTIGEFLEIIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLAAATEALKPGGRLVVITYH
SLEDRMVKNIMKTGNVEGKAETDFFGNLQTPFRLVNNKVIVPDEAEIERNPRSRSAKLRIAEKK

Sequences:

>Translated_304_residues
MEKDELTYHVPVLLKESVDGMNIHPDGTYVDVTFGGAGHSREILSRLGEGGRLLGFDQDEDAERNIVNDPHFTFVRSNFR
YLQNFLRYHDIEQVDAILADLGVSSHHFDDSERGFSFRFDGALDMRMNKRAGLTAADIVNTYEEERLANIFYLYGELKNS
RKLASAIVKARNGQSIRTIGEFLEIIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLAAATEALKPGGRLVVITYH
SLEDRMVKNIMKTGNVEGKAETDFFGNLQTPFRLVNNKVIVPDEAEIERNPRSRSAKLRIAEKK
>Mature_304_residues
MEKDELTYHVPVLLKESVDGMNIHPDGTYVDVTFGGAGHSREILSRLGEGGRLLGFDQDEDAERNIVNDPHFTFVRSNFR
YLQNFLRYHDIEQVDAILADLGVSSHHFDDSERGFSFRFDGALDMRMNKRAGLTAADIVNTYEEERLANIFYLYGELKNS
RKLASAIVKARNGQSIRTIGEFLEIIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLAAATEALKPGGRLVVITYH
SLEDRMVKNIMKTGNVEGKAETDFFGNLQTPFRLVNNKVIVPDEAEIERNPRSRSAKLRIAEKK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=33.9285714285714, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=33.5106382978723, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786270, Length=306, Percent_Identity=40.5228758169935, Blast_Score=220, Evalue=9e-59,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=36.1702127659575, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=36.1702127659575, Blast_Score=172, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34610; Mature: 34610

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDELTYHVPVLLKESVDGMNIHPDGTYVDVTFGGAGHSREILSRLGEGGRLLGFDQDE
CCCCCEEEEECCCEECCCCCCEECCCCCEEEEEECCCCCHHHHHHHCCCCCEEECCCCCC
DAERNIVNDPHFTFVRSNFRYLQNFLRYHDIEQVDAILADLGVSSHHFDDSERGFSFRFD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
GALDMRMNKRAGLTAADIVNTYEEERLANIFYLYGELKNSRKLASAIVKARNGQSIRTIG
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
EFLEIIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLAAATEALKPGGRLVVITYH
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SLEDRMVKNIMKTGNVEGKAETDFFGNLQTPFRLVNNKVIVPDEAEIERNPRSRSAKLRI
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCHHHCCCCCCCCCEEEE
AEKK
ECCC
>Mature Secondary Structure
MEKDELTYHVPVLLKESVDGMNIHPDGTYVDVTFGGAGHSREILSRLGEGGRLLGFDQDE
CCCCCEEEEECCCEECCCCCCEECCCCCEEEEEECCCCCHHHHHHHCCCCCEEECCCCCC
DAERNIVNDPHFTFVRSNFRYLQNFLRYHDIEQVDAILADLGVSSHHFDDSERGFSFRFD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
GALDMRMNKRAGLTAADIVNTYEEERLANIFYLYGELKNSRKLASAIVKARNGQSIRTIG
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
EFLEIIKPLFGREREKKELAKVFQALRIEVNQEMEALKEMLAAATEALKPGGRLVVITYH
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SLEDRMVKNIMKTGNVEGKAETDFFGNLQTPFRLVNNKVIVPDEAEIERNPRSRSAKLRI
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCHHHCCCCCCCCCEEEE
AEKK
ECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA