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The map label for this gene is mdtE [H]

Identifier: 29348748

GI number: 29348748

Start: 4299953

End: 4301128

Strand: Reverse

Name: mdtE [H]

Synonym: BT_3339

Alternate gene names: 29348748

Gene position: 4301128-4299953 (Counterclockwise)

Preceding gene: 29348749

Following gene: 29348747

Centisome position: 68.7

GC content: 43.62

Gene sequence:

>1176_bases
ATGAAAAGTAGAATTGTTTTTTTTGCATTTTGCTTAGCCCTCTTGTCTAGTTGCGGCAATAAGGGCAATGACACAGGGAA
AGTCCCAGAATATGCAGTACAAGAATTACAGAAGACAACTGCAAATCTAATGAAAGCTTATCCGGCTACTATCAAAGGTA
GACAAGACGTAGAAATCCGCCCGCAGGTATCGGGATTCATCACAAAGTTATGTGTAGATGAAGGAGCAACCGTTCGCAAA
GGACAGCTCTTATTCATCATAGACCCTACTCAGTATGAAGCAGCAGTACGCACAGCAAAAGCTTCAGTTGCAACAGCAGA
AGCAGCCGTGAATACACAACAAATGACTGTTGACAATAAGATCGAACTGAACAAGAAACAGATTATCAGCGATTATGACT
TGTCAATGGCGAAGAACTCACTGGCACAGGCTCAGGCACAACTCGCCCAGGCTAAAGCACAGCTGACCACAGCACAACAA
AATTACTCTTTCACACAAGTAAAGAGTCCTTCAGACGGTGTTATCAACGACATTCCTTATCGTTTGGGAGCACTCGTAAG
CCCCTCTATGGCTACACCAATGACTACAGTATCTGAAATAGACGAAGTATATGTATACTTCTCTACAACTGAAAAAGAAC
TGCTGGCTATGACCAAAACCGGAGGAACCATCAAAGAAGAAATCAGTAAAATACCAGCCATCAAACTACAATTGATTGAC
GGCACAACATATGATGCAGAAGGAAAGGTAGACGCTATCACAGGAGTGATCGACCAGTCAACCGGTTCTGTTAGCATGCG
TGCTATCTTCCCTAACAAGGAGCATATGCTTCGTAGTGGTGGAACTGCCAACGTTCTGATCCCTTATAATATGGAGAACG
TTATCAGCATCCCACAATCGGCTACTGTAGAAATCCAGGACAAAAAGTTCGTTTACGTTCTGCAGCCGGACAACACGGTG
AAATATACCGAGATCGGTATATTCAACCTGGACAACGGAAAAGAATATCTGGTAACTTCCGGACTGAACCCCGGCGATAA
AATCGTAATCGAAGGTGTACAGAGCCTGAAGGACGGCCAAAAAATACAACCAATCACGCCGGCTCAAAAAGAAGCGAACT
ATCAACAGCATTTGAAAGACCAGCACGACGGAAATTTAGCTACAGCTTTCAACTAA

Upstream 100 bases:

>100_bases
TGGAATAAGTTTCCGGCTTTTCTTCCTTAGATCATCTATCCATACAATGAATGTACACAGCGACGAACGCACAATTTTAA
AAAACAGATAAATATAAAGT

Downstream 100 bases:

>100_bases
TAATTTAGATAATCGTATGAAATTAGATAAATTCATTAACCGTCCGGTACTATCAACAGTTATTTCCATTTTGATAGTTA
TCCTAGGTGCTATCGGATTG

Product: AcrA/AcrE family multidrug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKSRIVFFAFCLALLSSCGNKGNDTGKVPEYAVQELQKTTANLMKAYPATIKGRQDVEIRPQVSGFITKLCVDEGATVRK
GQLLFIIDPTQYEAAVRTAKASVATAEAAVNTQQMTVDNKIELNKKQIISDYDLSMAKNSLAQAQAQLAQAKAQLTTAQQ
NYSFTQVKSPSDGVINDIPYRLGALVSPSMATPMTTVSEIDEVYVYFSTTEKELLAMTKTGGTIKEEISKIPAIKLQLID
GTTYDAEGKVDAITGVIDQSTGSVSMRAIFPNKEHMLRSGGTANVLIPYNMENVISIPQSATVEIQDKKFVYVLQPDNTV
KYTEIGIFNLDNGKEYLVTSGLNPGDKIVIEGVQSLKDGQKIQPITPAQKEANYQQHLKDQHDGNLATAFN

Sequences:

>Translated_391_residues
MKSRIVFFAFCLALLSSCGNKGNDTGKVPEYAVQELQKTTANLMKAYPATIKGRQDVEIRPQVSGFITKLCVDEGATVRK
GQLLFIIDPTQYEAAVRTAKASVATAEAAVNTQQMTVDNKIELNKKQIISDYDLSMAKNSLAQAQAQLAQAKAQLTTAQQ
NYSFTQVKSPSDGVINDIPYRLGALVSPSMATPMTTVSEIDEVYVYFSTTEKELLAMTKTGGTIKEEISKIPAIKLQLID
GTTYDAEGKVDAITGVIDQSTGSVSMRAIFPNKEHMLRSGGTANVLIPYNMENVISIPQSATVEIQDKKFVYVLQPDNTV
KYTEIGIFNLDNGKEYLVTSGLNPGDKIVIEGVQSLKDGQKIQPITPAQKEANYQQHLKDQHDGNLATAFN
>Mature_391_residues
MKSRIVFFAFCLALLSSCGNKGNDTGKVPEYAVQELQKTTANLMKAYPATIKGRQDVEIRPQVSGFITKLCVDEGATVRK
GQLLFIIDPTQYEAAVRTAKASVATAEAAVNTQQMTVDNKIELNKKQIISDYDLSMAKNSLAQAQAQLAQAKAQLTTAQQ
NYSFTQVKSPSDGVINDIPYRLGALVSPSMATPMTTVSEIDEVYVYFSTTEKELLAMTKTGGTIKEEISKIPAIKLQLID
GTTYDAEGKVDAITGVIDQSTGSVSMRAIFPNKEHMLRSGGTANVLIPYNMENVISIPQSATVEIQDKKFVYVLQPDNTV
KYTEIGIFNLDNGKEYLVTSGLNPGDKIVIEGVQSLKDGQKIQPITPAQKEANYQQHLKDQHDGNLATAFN

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=377, Percent_Identity=31.0344827586207, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1786668, Length=367, Percent_Identity=31.6076294277929, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789665, Length=372, Percent_Identity=28.2258064516129, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082036, Length=330, Percent_Identity=21.8181818181818, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081796, Length=344, Percent_Identity=26.1627906976744, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789637, Length=131, Percent_Identity=30.5343511450382, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42650; Mature: 42650

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRIVFFAFCLALLSSCGNKGNDTGKVPEYAVQELQKTTANLMKAYPATIKGRQDVEIR
CCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
PQVSGFITKLCVDEGATVRKGQLLFIIDPTQYEAAVRTAKASVATAEAAVNTQQMTVDNK
CHHHHHHHHHHHCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCE
IELNKKQIISDYDLSMAKNSLAQAQAQLAQAKAQLTTAQQNYSFTQVKSPSDGVINDIPY
EECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCH
RLGALVSPSMATPMTTVSEIDEVYVYFSTTEKELLAMTKTGGTIKEEISKIPAIKLQLID
HHHHHCCCCCCCCCHHHHCCCEEEEEEECCCCEEEEEECCCCHHHHHHHCCCEEEEEEEE
GTTYDAEGKVDAITGVIDQSTGSVSMRAIFPNKEHMLRSGGTANVLIPYNMENVISIPQS
CCEECCCCCEEEEEEEEECCCCCEEEEEECCCHHHHHHCCCCEEEEEECCCHHHEECCCC
ATVEIQDKKFVYVLQPDNTVKYTEIGIFNLDNGKEYLVTSGLNPGDKIVIEGVQSLKDGQ
CEEEEECCEEEEEECCCCEEEEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
KIQPITPAQKEANYQQHLKDQHDGNLATAFN
CCCCCCCCHHHCCHHHHHHHCCCCCEEECCC
>Mature Secondary Structure
MKSRIVFFAFCLALLSSCGNKGNDTGKVPEYAVQELQKTTANLMKAYPATIKGRQDVEIR
CCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
PQVSGFITKLCVDEGATVRKGQLLFIIDPTQYEAAVRTAKASVATAEAAVNTQQMTVDNK
CHHHHHHHHHHHCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCE
IELNKKQIISDYDLSMAKNSLAQAQAQLAQAKAQLTTAQQNYSFTQVKSPSDGVINDIPY
EECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCH
RLGALVSPSMATPMTTVSEIDEVYVYFSTTEKELLAMTKTGGTIKEEISKIPAIKLQLID
HHHHHCCCCCCCCCHHHHCCCEEEEEEECCCCEEEEEECCCCHHHHHHHCCCEEEEEEEE
GTTYDAEGKVDAITGVIDQSTGSVSMRAIFPNKEHMLRSGGTANVLIPYNMENVISIPQS
CCEECCCCCEEEEEEEEECCCCCEEEEEECCCHHHHHHCCCCEEEEEECCCHHHEECCCC
ATVEIQDKKFVYVLQPDNTVKYTEIGIFNLDNGKEYLVTSGLNPGDKIVIEGVQSLKDGQ
CEEEEECCEEEEEECCCCEEEEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
KIQPITPAQKEANYQQHLKDQHDGNLATAFN
CCCCCCCCHHHCCHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]