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The map label for this gene is lacZ [H]

Identifier: 29348701

GI number: 29348701

Start: 4212870

End: 4214645

Strand: Reverse

Name: lacZ [H]

Synonym: BT_3292

Alternate gene names: 29348701

Gene position: 4214645-4212870 (Counterclockwise)

Preceding gene: 29348703

Following gene: 29348699

Centisome position: 67.32

GC content: 44.2

Gene sequence:

>1776_bases
ATGAGAAAAGCACATCTGATACTAGTTGGTTTACTACTCTCTTTTGCAGCAAACGCAACCAATGATATTCCTCGGCCGGA
ATATCCCCGCCCACAATTTGAAAGAACTACCTGGGTTAATTTAAATGGTACGTGGACATATGAATTCGATCTTGATGATT
CCGGGAAAAAGCGTAACTTACCTACGGCAAAGGAGCTTAGCAAAACAATTACGGTTCCTTTCTGCCCGGAAAGCAAACTA
TCCGGAGTTAATCACACGGACTTTATAAAAAAAATGTGGTATCAACGTTCTCTGCCGATACCCGCTGACTGGAGCAACAA
GAAGATTTTACTCCATTTCGGCGCTGTCGACTATTTAGCAGAGATTTATATAGACGGACGCCTCGTCGGTTTTCATAACG
GCGGAAGCTCTCCTTTTGTAATAGATATCAGTCGCATAGCCAAACCCGGGAATAGTCACAATCTGGTAGTATCCGTGTCC
GATGATGCAAAATCAGGTCGTCAGGCATGCGGCAAACAGTCTCCGGAGAAAAACTCCTTTGCCTGTTTTTATACGCGTGT
CACAGGTATCTGGCAAACGGTGTGGATGGAAGCTCTCTCTCCCTGTGGACTGAAATCGGCAAATACCTATCCGGATATTG
ACAATAATCAATTGATCATTACTCCGGAATTCTATCAGATATCCAATGACCAGACACTGGAAGTTACGATCTATGACAGC
CAGAAAAAAGTCGCTCAAGTGACATCAAAATGTGCCAATGGCAGTAACCTGATCTTACCGATAAAGAATATCAAGCTCTG
GAGCCCCGAAACTCCCCACCTTTACGATATCAGTTACTGCGTCAAAGATGCCAAAGGGCAGATCATCGATGAGGTAAAAT
CATATGTAGGTATGCGCAAAGTGCATATAGCCAATGGCAAATTTTACTTGAATAACGAGCCTTATTTCCAGCGTCTGGTT
ATGAATCAGGGGTATTATCCTGATGGAATCTGGACTGCACCTACCGATGAAGCCCTTAAAAATGATATTTTACTAAGTAA
GGAAGCCGGCTTCAACGGGGCACGGCTTCATCAAAAGTTTTTCGAAGAACGATTCCATTACTGGGCAGATAAGCTGGGAT
TTATCACTTGGGGAGAGTCTCCTAACTGGGGAATGAATCCCGATGACGAGGTCGCATCAAGAAATCTTCTCAGTGAATGG
ATAGAGATACTCGAACGAGACCGTAATCACCCTTCTATCATCACATGGGCACCTCTTACTGTACCATTGAGCAATGTTTC
GTCCGGTACATTTGCACGCCTGGTATTTGACCTGCAAAAACTGACAAAAGCAATCGATCCCAGTCGCCCGTTCAACGACC
TTACCGGCAGTGGATTCCATTTCCTGACAGATATCTGGAGCATCAGTACTTACGAACCGGATGCAACACGTTTCGCACTA
AGCTTAAAGCCGGACAAGAACCAGGCAGCTTATGCCAACCAACCTTTTATCATCGGTGAATTCGGCGGTATTGTATGGGA
AACATCCAGAACCAAGGAAGACACATGGGGACATGGAGGAACATTTACCAACGAAGATGCCCTCTTCGAACGCATCGAAA
AACTCATCAATGCGATTCAATCCTCCGGAATCATCAGCGGATTCTGCTATACACAGTTTTCAGATATCGAACAAGAGAAA
AACGGTATCTACACTTACGACCGACAGCCTAAATTTGAGATGGAACGGATTAGATCTATCTTTGAAAAGATTCCAAGCAG
ACCGATAAAGAAATAA

Upstream 100 bases:

>100_bases
TCCTAATTTGAACAATCGTTTACACCTTTTCTTCTTTTAAATCTCTATTTTTACAATCAGCTAAGACACTTAAATTATAC
AATAACAAAAATTACAAAAC

Downstream 100 bases:

>100_bases
GTTCAGCTTGCTAATAGTATAATTCAGCCCGTCAATATATTCAAATCGAATTATATCGACGGGCTGAATATCCCTTTAAT
CAGGGTGAAACCTTACCGTT

Product: beta-glucuronidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRKAHLILVGLLLSFAANATNDIPRPEYPRPQFERTTWVNLNGTWTYEFDLDDSGKKRNLPTAKELSKTITVPFCPESKL
SGVNHTDFIKKMWYQRSLPIPADWSNKKILLHFGAVDYLAEIYIDGRLVGFHNGGSSPFVIDISRIAKPGNSHNLVVSVS
DDAKSGRQACGKQSPEKNSFACFYTRVTGIWQTVWMEALSPCGLKSANTYPDIDNNQLIITPEFYQISNDQTLEVTIYDS
QKKVAQVTSKCANGSNLILPIKNIKLWSPETPHLYDISYCVKDAKGQIIDEVKSYVGMRKVHIANGKFYLNNEPYFQRLV
MNQGYYPDGIWTAPTDEALKNDILLSKEAGFNGARLHQKFFEERFHYWADKLGFITWGESPNWGMNPDDEVASRNLLSEW
IEILERDRNHPSIITWAPLTVPLSNVSSGTFARLVFDLQKLTKAIDPSRPFNDLTGSGFHFLTDIWSISTYEPDATRFAL
SLKPDKNQAAYANQPFIIGEFGGIVWETSRTKEDTWGHGGTFTNEDALFERIEKLINAIQSSGIISGFCYTQFSDIEQEK
NGIYTYDRQPKFEMERIRSIFEKIPSRPIKK

Sequences:

>Translated_591_residues
MRKAHLILVGLLLSFAANATNDIPRPEYPRPQFERTTWVNLNGTWTYEFDLDDSGKKRNLPTAKELSKTITVPFCPESKL
SGVNHTDFIKKMWYQRSLPIPADWSNKKILLHFGAVDYLAEIYIDGRLVGFHNGGSSPFVIDISRIAKPGNSHNLVVSVS
DDAKSGRQACGKQSPEKNSFACFYTRVTGIWQTVWMEALSPCGLKSANTYPDIDNNQLIITPEFYQISNDQTLEVTIYDS
QKKVAQVTSKCANGSNLILPIKNIKLWSPETPHLYDISYCVKDAKGQIIDEVKSYVGMRKVHIANGKFYLNNEPYFQRLV
MNQGYYPDGIWTAPTDEALKNDILLSKEAGFNGARLHQKFFEERFHYWADKLGFITWGESPNWGMNPDDEVASRNLLSEW
IEILERDRNHPSIITWAPLTVPLSNVSSGTFARLVFDLQKLTKAIDPSRPFNDLTGSGFHFLTDIWSISTYEPDATRFAL
SLKPDKNQAAYANQPFIIGEFGGIVWETSRTKEDTWGHGGTFTNEDALFERIEKLINAIQSSGIISGFCYTQFSDIEQEK
NGIYTYDRQPKFEMERIRSIFEKIPSRPIKK
>Mature_591_residues
MRKAHLILVGLLLSFAANATNDIPRPEYPRPQFERTTWVNLNGTWTYEFDLDDSGKKRNLPTAKELSKTITVPFCPESKL
SGVNHTDFIKKMWYQRSLPIPADWSNKKILLHFGAVDYLAEIYIDGRLVGFHNGGSSPFVIDISRIAKPGNSHNLVVSVS
DDAKSGRQACGKQSPEKNSFACFYTRVTGIWQTVWMEALSPCGLKSANTYPDIDNNQLIITPEFYQISNDQTLEVTIYDS
QKKVAQVTSKCANGSNLILPIKNIKLWSPETPHLYDISYCVKDAKGQIIDEVKSYVGMRKVHIANGKFYLNNEPYFQRLV
MNQGYYPDGIWTAPTDEALKNDILLSKEAGFNGARLHQKFFEERFHYWADKLGFITWGESPNWGMNPDDEVASRNLLSEW
IEILERDRNHPSIITWAPLTVPLSNVSSGTFARLVFDLQKLTKAIDPSRPFNDLTGSGFHFLTDIWSISTYEPDATRFAL
SLKPDKNQAAYANQPFIIGEFGGIVWETSRTKEDTWGHGGTFTNEDALFERIEKLINAIQSSGIISGFCYTQFSDIEQEK
NGIYTYDRQPKFEMERIRSIFEKIPSRPIKK

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=462, Percent_Identity=22.0779220779221, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1787903, Length=584, Percent_Identity=22.6027397260274, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI48994920, Length=413, Percent_Identity=23.728813559322, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786539, Length=423, Percent_Identity=24.113475177305, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI62471735, Length=616, Percent_Identity=24.1883116883117, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19922582, Length=616, Percent_Identity=24.1883116883117, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24655438, Length=616, Percent_Identity=24.1883116883117, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 67264; Mature: 67264

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAHLILVGLLLSFAANATNDIPRPEYPRPQFERTTWVNLNGTWTYEFDLDDSGKKRNL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCC
PTAKELSKTITVPFCPESKLSGVNHTDFIKKMWYQRSLPIPADWSNKKILLHFGAVDYLA
CCHHHHHHEEEEECCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHH
EIYIDGRLVGFHNGGSSPFVIDISRIAKPGNSHNLVVSVSDDAKSGRQACGKQSPEKNSF
HHEECCEEEEEECCCCCCEEEEHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCE
ACFYTRVTGIWQTVWMEALSPCGLKSANTYPDIDNNQLIITPEFYQISNDQTLEVTIYDS
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEECCCCCEEEEEEECC
QKKVAQVTSKCANGSNLILPIKNIKLWSPETPHLYDISYCVKDAKGQIIDEVKSYVGMRK
HHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCEEEHHHHHHHHCCHHHHHHHHHHCCEE
VHIANGKFYLNNEPYFQRLVMNQGYYPDGIWTAPTDEALKNDILLSKEAGFNGARLHQKF
EEEECCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCEEEECCCCCCHHHHHHHH
FEERFHYWADKLGFITWGESPNWGMNPDDEVASRNLLSEWIEILERDRNHPSIITWAPLT
HHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEE
VPLSNVSSGTFARLVFDLQKLTKAIDPSRPFNDLTGSGFHFLTDIWSISTYEPDATRFAL
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
SLKPDKNQAAYANQPFIIGEFGGIVWETSRTKEDTWGHGGTFTNEDALFERIEKLINAIQ
EECCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SSGIISGFCYTQFSDIEQEKNGIYTYDRQPKFEMERIRSIFEKIPSRPIKK
HCCCEEEEHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRKAHLILVGLLLSFAANATNDIPRPEYPRPQFERTTWVNLNGTWTYEFDLDDSGKKRNL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCC
PTAKELSKTITVPFCPESKLSGVNHTDFIKKMWYQRSLPIPADWSNKKILLHFGAVDYLA
CCHHHHHHEEEEECCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHH
EIYIDGRLVGFHNGGSSPFVIDISRIAKPGNSHNLVVSVSDDAKSGRQACGKQSPEKNSF
HHEECCEEEEEECCCCCCEEEEHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCE
ACFYTRVTGIWQTVWMEALSPCGLKSANTYPDIDNNQLIITPEFYQISNDQTLEVTIYDS
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEECCCCCEEEEEEECC
QKKVAQVTSKCANGSNLILPIKNIKLWSPETPHLYDISYCVKDAKGQIIDEVKSYVGMRK
HHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCEEEHHHHHHHHCCHHHHHHHHHHCCEE
VHIANGKFYLNNEPYFQRLVMNQGYYPDGIWTAPTDEALKNDILLSKEAGFNGARLHQKF
EEEECCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCEEEECCCCCCHHHHHHHH
FEERFHYWADKLGFITWGESPNWGMNPDDEVASRNLLSEWIEILERDRNHPSIITWAPLT
HHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEE
VPLSNVSSGTFARLVFDLQKLTKAIDPSRPFNDLTGSGFHFLTDIWSISTYEPDATRFAL
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEE
SLKPDKNQAAYANQPFIIGEFGGIVWETSRTKEDTWGHGGTFTNEDALFERIEKLINAIQ
EECCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SSGIISGFCYTQFSDIEQEKNGIYTYDRQPKFEMERIRSIFEKIPSRPIKK
HCCCEEEEHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA