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The map label for this gene is mnmC [H]

Identifier: 29348659

GI number: 29348659

Start: 4149923

End: 4150645

Strand: Reverse

Name: mnmC [H]

Synonym: BT_3250

Alternate gene names: 29348659

Gene position: 4150645-4149923 (Counterclockwise)

Preceding gene: 29348660

Following gene: 29348658

Centisome position: 66.3

GC content: 47.03

Gene sequence:

>723_bases
ATGAAAAGAGTGATTGAAAAGACTGATGACGGGAGCGCCACCCTATTTGTTCCGGAATTGAATGAACATTATCATTCGAC
CAAAGGAGCACGTACGGAGTCCCAGCATATTTTCATCAATATGGGACTGAAAGCCTCCGCTGCCCCCTCTCCGCATATTC
TGGAGATCGGTTTCGGAACGGGGCTGAATGCTTGGCTTACACTTGAAGAAGCAGAAAGAAGCGGACGTAACGTACATTAT
ACAGGACTTGAACCCTACCCGTTGGAATGGCAGATGGTGGAGCAACTGGGATATATAGAACAACCAGCAGCTATCGACCT
GTTCAGAGCAATCCATACATCACCGTGGGAAGAAGAAATATCCTTTACCCCACACTTCACTCTCTGCAAAGTACAAGCCG
ACGTCAATGAATGGATTACCCAGCGCAGCCATTCTCCATTATCCATTATCCATTCTCCATTATCATTCGACGTTATCTAT
TTCGACGCCTTCGCTCCGGAAAAGCAGCCGGAAATGTGGTCGCAGGAACTATTTAACCGTTTATATGTTTTATTAAATGA
AGGAGGCATCCTCACCACTTATTGTGCTAAAGGAGTCATCCGACGTATGCTGCAGGCCGCAGGCTTTATCGTAGAGCGCC
TTCCCGGTCCTCCGGGAGGAAAGCGGGAAATACTACGTGCCCGAAAACTGAAACAGAATCAACCCCCGATATTAATACAA
TAG

Upstream 100 bases:

>100_bases
TACATTCTGCTTCTGTATGTACTGGAAAGCGCATGGAACGTCGTTGCAGGATGAAATAATAGGTAATAAGTAACAGGTAA
TAGGTAATAAGTTTTTTGAG

Downstream 100 bases:

>100_bases
TCATGAAACAACAACTAAAAGATTTGAAAACACTCCTTCTTCTCGGCTCCTGCCTCCTGTTGGCAGCCTGCACGGGTCGC
ACGTCCAAAGCAAGCGGCAG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKRVIEKTDDGSATLFVPELNEHYHSTKGARTESQHIFINMGLKASAAPSPHILEIGFGTGLNAWLTLEEAERSGRNVHY
TGLEPYPLEWQMVEQLGYIEQPAAIDLFRAIHTSPWEEEISFTPHFTLCKVQADVNEWITQRSHSPLSIIHSPLSFDVIY
FDAFAPEKQPEMWSQELFNRLYVLLNEGGILTTYCAKGVIRRMLQAAGFIVERLPGPPGGKREILRARKLKQNQPPILIQ

Sequences:

>Translated_240_residues
MKRVIEKTDDGSATLFVPELNEHYHSTKGARTESQHIFINMGLKASAAPSPHILEIGFGTGLNAWLTLEEAERSGRNVHY
TGLEPYPLEWQMVEQLGYIEQPAAIDLFRAIHTSPWEEEISFTPHFTLCKVQADVNEWITQRSHSPLSIIHSPLSFDVIY
FDAFAPEKQPEMWSQELFNRLYVLLNEGGILTTYCAKGVIRRMLQAAGFIVERLPGPPGGKREILRARKLKQNQPPILIQ
>Mature_240_residues
MKRVIEKTDDGSATLFVPELNEHYHSTKGARTESQHIFINMGLKASAAPSPHILEIGFGTGLNAWLTLEEAERSGRNVHY
TGLEPYPLEWQMVEQLGYIEQPAAIDLFRAIHTSPWEEEISFTPHFTLCKVQADVNEWITQRSHSPLSIIHSPLSFDVIY
FDAFAPEKQPEMWSQELFNRLYVLLNEGGILTTYCAKGVIRRMLQAAGFIVERLPGPPGGKREILRARKLKQNQPPILIQ

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=236, Percent_Identity=28.8135593220339, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 27264; Mature: 27264

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVIEKTDDGSATLFVPELNEHYHSTKGARTESQHIFINMGLKASAAPSPHILEIGFGT
CCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCC
GLNAWLTLEEAERSGRNVHYTGLEPYPLEWQMVEQLGYIEQPAAIDLFRAIHTSPWEEEI
CCCEEEEEEHHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHC
SFTPHFTLCKVQADVNEWITQRSHSPLSIIHSPLSFDVIYFDAFAPEKQPEMWSQELFNR
CCCCCEEEEEEECCHHHHHHHCCCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHH
LYVLLNEGGILTTYCAKGVIRRMLQAAGFIVERLPGPPGGKREILRARKLKQNQPPILIQ
HEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MKRVIEKTDDGSATLFVPELNEHYHSTKGARTESQHIFINMGLKASAAPSPHILEIGFGT
CCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCC
GLNAWLTLEEAERSGRNVHYTGLEPYPLEWQMVEQLGYIEQPAAIDLFRAIHTSPWEEEI
CCCEEEEEEHHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHC
SFTPHFTLCKVQADVNEWITQRSHSPLSIIHSPLSFDVIYFDAFAPEKQPEMWSQELFNR
CCCCCEEEEEEECCHHHHHHHCCCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHH
LYVLLNEGGILTTYCAKGVIRRMLQAAGFIVERLPGPPGGKREILRARKLKQNQPPILIQ
HEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA