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The map label for this gene is pflD [H]

Identifier: 29348364

GI number: 29348364

Start: 3721561

End: 3723705

Strand: Reverse

Name: pflD [H]

Synonym: BT_2955

Alternate gene names: 29348364

Gene position: 3723705-3721561 (Counterclockwise)

Preceding gene: 29348365

Following gene: 29348363

Centisome position: 59.48

GC content: 43.68

Gene sequence:

>2145_bases
ATGAAGAATTACGAGCAAAAAATAGCGAATCTGCGTATGCGGAAACTCGCTCAAACTCAGGAAAAGATTGAAAAGGAAGG
ACTTCTGGACGAAGATGACTATGGACGGGTGGTTCCTCCGGAAAATTTGTGGAATATCATACCTAACCATCCCGATGGTT
CTTTCTACGGATTTGATGCATGGGCTGATAACTTTTGCAGCCTGATGAATATTCATCCGGTATATATTGATGCCGATGAT
GCTTTTGCAGGAAGATGGATGTATTTCATGTCTAAAATGCGACCCAATAAATGGAATCCGGATTATTCTTATGACTTTCT
AAAAGAGAATATCAAGAAGTATGACCTCATTTGTGGTATTGGCGATGACGCTCATTTCGCTCCTGATTATGAAATCGGGG
TAAAATTAGGATGGAATGGCTTGATAAAGAAGATAGAACATTACCAATCCATGCATCATTCAGAAGAGCAACAACACTTC
TACAGCTTGCATCTAAGGGTCATACGTAGTGTGCAGGGGTGGATACAGCGTCATATCGACCAGGCATATAGAATGGCTGC
ATCTGCAACAGACGATTGTTCTAAAAACAACTTACTGGAAATAGCCAAAGTAAACGAGAGCATTATCAATGATGCCCCAA
CGACATTAAGAGAAGCCTGTCAGTGGATCATATGGTATCATCTTGCATCAAGAACTTACAACCGTGATGGAGCTGGCGGA
CAAATTGATACTTTATTGGCTCCTTTTTATGAAAAAGATTTGGCAGAAGGAAGGATTGATAAAGAAACGGCCGTTTATTA
TTTGGCGTGTTTTTTACTGAACGACCCTGTCTACTGGCAATTGGGAGGTCCCGACGAATCAGGTAAAGACCAAACTTCCC
CCCTTTCATTCTTAATACTGGAAGCTGCCGATAAAATCAATACCTCTTTAAATCTGACAGTGAGAGTGTTCGATGGTCTG
AACAAGGACTTATTCCGGAAATCCGTAGAGTACCTGGTTAAGAACAAGAATGGATGGCCCAGATTCTCAGGAGATAAAGC
ATTGGTAGAAGGATTTATGAAGAACGGCTTCAGTGCACAACTGGCTCGCCGTCGTATAGCAGTGGGATGCAACTGGATGT
CGCTGCCCGGACTGGAATATACAATGAATGACCTTGTGAAAATAAATATGGCGAAAGTTTTTGAGGTATCCTATACGGAA
ATGATGAATGAACAAAAAGATCATAGCACCTCCCGGCTCTGGATGTTATTCAACGAGCATTTGGCTAAGGCAGTCCAAAC
ATCAGCCGACGGAATTCGCCATCACCTGAAATTTCAAAAATACAATGAGCCGGAGTTATTACTAAATCTATTGAGCCACG
GGCCATTGGAAAAAGGCAAAGACGTATCGGACGGTGGAGCGGAATACTACAACCTGGCAATTGACGGAGCCGGATTGGCT
ACTGTCGCTGACTCTTTTGCCGCTTTGGAACAGCGCATCGAGTTGGAAAACAAATTGTCCTGGAATACCCTGACCGAATA
TCTGAGAAACAATTTCTCAGGAGACGGAGGATGCAGAATACGGCATTTATTGAAGAACAGCGAAAAATATGGAGCCGCCG
AATCCTTGGGCGAAAAGTGGGCGATTCGGATCAAAGAAACATTTACAGAACTGGTACGTTTGCAGTCCGAACCGGACAAA
CGCCGGATTTTTATTCCCGGCTTCTTCTCTTGGGCAAATACGGTAGGATTCGGACAAAGTGTGGGAGCTACTCCCGACGG
ACGTTTAGCGCAAACACCTATCTCTCATGGAGCAAATCCCACTCCCGGTTTTGTAAAAGACGGCGCATCGCTTTCACTTG
CTACCATTATAGCTAAAATTCAGCCGGGATATGGCAATACGGCACCCATGCAATGGGAGCTGGACCCTACATTCGCCAAT
ATGGATAATATAGAGTTAATCATGTCCATCATCAGGGCACACTTCGACTTGGGAGGTACTTTGATTAACGTCAATATTAT
GAATAAGGATACTGTATTGGCTGCCCATAAAGATCCTGCCGGATATCCGGAACTGGTAGTCCGTGTTACCGGATTTACAG
CCTACTTTTCCATGTTGTCCCCTCAATTCAGACAATTGGTAGTAGACCGTATCTTAGAAAGCTAA

Upstream 100 bases:

>100_bases
GAATGAAGAATCCCTTAAGCAATTCTGCTTTTTTAAAGAAAGGACAATTACAAAAACTAAAGGAAATACTGATCAGATAT
AATTTAAACAATAACAAAAA

Downstream 100 bases:

>100_bases
ATCATATTCACTCTAAAAATCTTATATCATGAAAATCATTGATGAACACACATTTGAAGGAAAGGGATATCACCCGTTCC
TGATAACCGACAGGTGGCAG

Product: formate acetyltransferase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKNYEQKIANLRMRKLAQTQEKIEKEGLLDEDDYGRVVPPENLWNIIPNHPDGSFYGFDAWADNFCSLMNIHPVYIDADD
AFAGRWMYFMSKMRPNKWNPDYSYDFLKENIKKYDLICGIGDDAHFAPDYEIGVKLGWNGLIKKIEHYQSMHHSEEQQHF
YSLHLRVIRSVQGWIQRHIDQAYRMAASATDDCSKNNLLEIAKVNESIINDAPTTLREACQWIIWYHLASRTYNRDGAGG
QIDTLLAPFYEKDLAEGRIDKETAVYYLACFLLNDPVYWQLGGPDESGKDQTSPLSFLILEAADKINTSLNLTVRVFDGL
NKDLFRKSVEYLVKNKNGWPRFSGDKALVEGFMKNGFSAQLARRRIAVGCNWMSLPGLEYTMNDLVKINMAKVFEVSYTE
MMNEQKDHSTSRLWMLFNEHLAKAVQTSADGIRHHLKFQKYNEPELLLNLLSHGPLEKGKDVSDGGAEYYNLAIDGAGLA
TVADSFAALEQRIELENKLSWNTLTEYLRNNFSGDGGCRIRHLLKNSEKYGAAESLGEKWAIRIKETFTELVRLQSEPDK
RRIFIPGFFSWANTVGFGQSVGATPDGRLAQTPISHGANPTPGFVKDGASLSLATIIAKIQPGYGNTAPMQWELDPTFAN
MDNIELIMSIIRAHFDLGGTLINVNIMNKDTVLAAHKDPAGYPELVVRVTGFTAYFSMLSPQFRQLVVDRILES

Sequences:

>Translated_714_residues
MKNYEQKIANLRMRKLAQTQEKIEKEGLLDEDDYGRVVPPENLWNIIPNHPDGSFYGFDAWADNFCSLMNIHPVYIDADD
AFAGRWMYFMSKMRPNKWNPDYSYDFLKENIKKYDLICGIGDDAHFAPDYEIGVKLGWNGLIKKIEHYQSMHHSEEQQHF
YSLHLRVIRSVQGWIQRHIDQAYRMAASATDDCSKNNLLEIAKVNESIINDAPTTLREACQWIIWYHLASRTYNRDGAGG
QIDTLLAPFYEKDLAEGRIDKETAVYYLACFLLNDPVYWQLGGPDESGKDQTSPLSFLILEAADKINTSLNLTVRVFDGL
NKDLFRKSVEYLVKNKNGWPRFSGDKALVEGFMKNGFSAQLARRRIAVGCNWMSLPGLEYTMNDLVKINMAKVFEVSYTE
MMNEQKDHSTSRLWMLFNEHLAKAVQTSADGIRHHLKFQKYNEPELLLNLLSHGPLEKGKDVSDGGAEYYNLAIDGAGLA
TVADSFAALEQRIELENKLSWNTLTEYLRNNFSGDGGCRIRHLLKNSEKYGAAESLGEKWAIRIKETFTELVRLQSEPDK
RRIFIPGFFSWANTVGFGQSVGATPDGRLAQTPISHGANPTPGFVKDGASLSLATIIAKIQPGYGNTAPMQWELDPTFAN
MDNIELIMSIIRAHFDLGGTLINVNIMNKDTVLAAHKDPAGYPELVVRVTGFTAYFSMLSPQFRQLVVDRILES
>Mature_714_residues
MKNYEQKIANLRMRKLAQTQEKIEKEGLLDEDDYGRVVPPENLWNIIPNHPDGSFYGFDAWADNFCSLMNIHPVYIDADD
AFAGRWMYFMSKMRPNKWNPDYSYDFLKENIKKYDLICGIGDDAHFAPDYEIGVKLGWNGLIKKIEHYQSMHHSEEQQHF
YSLHLRVIRSVQGWIQRHIDQAYRMAASATDDCSKNNLLEIAKVNESIINDAPTTLREACQWIIWYHLASRTYNRDGAGG
QIDTLLAPFYEKDLAEGRIDKETAVYYLACFLLNDPVYWQLGGPDESGKDQTSPLSFLILEAADKINTSLNLTVRVFDGL
NKDLFRKSVEYLVKNKNGWPRFSGDKALVEGFMKNGFSAQLARRRIAVGCNWMSLPGLEYTMNDLVKINMAKVFEVSYTE
MMNEQKDHSTSRLWMLFNEHLAKAVQTSADGIRHHLKFQKYNEPELLLNLLSHGPLEKGKDVSDGGAEYYNLAIDGAGLA
TVADSFAALEQRIELENKLSWNTLTEYLRNNFSGDGGCRIRHLLKNSEKYGAAESLGEKWAIRIKETFTELVRLQSEPDK
RRIFIPGFFSWANTVGFGQSVGATPDGRLAQTPISHGANPTPGFVKDGASLSLATIIAKIQPGYGNTAPMQWELDPTFAN
MDNIELIMSIIRAHFDLGGTLINVNIMNKDTVLAAHKDPAGYPELVVRVTGFTAYFSMLSPQFRQLVVDRILES

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=595, Percent_Identity=26.890756302521, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1787044, Length=649, Percent_Identity=24.3451463790447, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI48994926, Length=256, Percent_Identity=27.734375, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787131, Length=258, Percent_Identity=27.5193798449612, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 81023; Mature: 81023

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYEQKIANLRMRKLAQTQEKIEKEGLLDEDDYGRVVPPENLWNIIPNHPDGSFYGFDA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEECHH
WADNFCSLMNIHPVYIDADDAFAGRWMYFMSKMRPNKWNPDYSYDFLKENIKKYDLICGI
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEEC
GDDAHFAPDYEIGVKLGWNGLIKKIEHYQSMHHSEEQQHFYSLHLRVIRSVQGWIQRHID
CCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QAYRMAASATDDCSKNNLLEIAKVNESIINDAPTTLREACQWIIWYHLASRTYNRDGAGG
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
QIDTLLAPFYEKDLAEGRIDKETAVYYLACFLLNDPVYWQLGGPDESGKDQTSPLSFLIL
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHEE
EAADKINTSLNLTVRVFDGLNKDLFRKSVEYLVKNKNGWPRFSGDKALVEGFMKNGFSAQ
HHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHH
LARRRIAVGCNWMSLPGLEYTMNDLVKINMAKVFEVSYTEMMNEQKDHSTSRLWMLFNEH
HHHHHHHCCCCEEECCCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAKAVQTSADGIRHHLKFQKYNEPELLLNLLSHGPLEKGKDVSDGGAEYYNLAIDGAGLA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHH
TVADSFAALEQRIELENKLSWNTLTEYLRNNFSGDGGCRIRHLLKNSEKYGAAESLGEKW
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHCCCHH
AIRIKETFTELVRLQSEPDKRRIFIPGFFSWANTVGFGQSVGATPDGRLAQTPISHGANP
EEHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCC
TPGFVKDGASLSLATIIAKIQPGYGNTAPMQWELDPTFANMDNIELIMSIIRAHFDLGGT
CCCHHCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCE
LINVNIMNKDTVLAAHKDPAGYPELVVRVTGFTAYFSMLSPQFRQLVVDRILES
EEEEEEECCCEEEEECCCCCCCHHHEEEHHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNYEQKIANLRMRKLAQTQEKIEKEGLLDEDDYGRVVPPENLWNIIPNHPDGSFYGFDA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEECHH
WADNFCSLMNIHPVYIDADDAFAGRWMYFMSKMRPNKWNPDYSYDFLKENIKKYDLICGI
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEEC
GDDAHFAPDYEIGVKLGWNGLIKKIEHYQSMHHSEEQQHFYSLHLRVIRSVQGWIQRHID
CCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QAYRMAASATDDCSKNNLLEIAKVNESIINDAPTTLREACQWIIWYHLASRTYNRDGAGG
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
QIDTLLAPFYEKDLAEGRIDKETAVYYLACFLLNDPVYWQLGGPDESGKDQTSPLSFLIL
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHEE
EAADKINTSLNLTVRVFDGLNKDLFRKSVEYLVKNKNGWPRFSGDKALVEGFMKNGFSAQ
HHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHH
LARRRIAVGCNWMSLPGLEYTMNDLVKINMAKVFEVSYTEMMNEQKDHSTSRLWMLFNEH
HHHHHHHCCCCEEECCCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAKAVQTSADGIRHHLKFQKYNEPELLLNLLSHGPLEKGKDVSDGGAEYYNLAIDGAGLA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHH
TVADSFAALEQRIELENKLSWNTLTEYLRNNFSGDGGCRIRHLLKNSEKYGAAESLGEKW
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHCCCHH
AIRIKETFTELVRLQSEPDKRRIFIPGFFSWANTVGFGQSVGATPDGRLAQTPISHGANP
EEHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCC
TPGFVKDGASLSLATIIAKIQPGYGNTAPMQWELDPTFANMDNIELIMSIIRAHFDLGGT
CCCHHCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCE
LINVNIMNKDTVLAAHKDPAGYPELVVRVTGFTAYFSMLSPQFRQLVVDRILES
EEEEEEECCCEEEEECCCCCCCHHHEEEHHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]