Click here to switch to the map view.

The map label for this gene is lacZ [H]

Identifier: 29348331

GI number: 29348331

Start: 3667119

End: 3668933

Strand: Reverse

Name: lacZ [H]

Synonym: BT_2922

Alternate gene names: 29348331

Gene position: 3668933-3667119 (Counterclockwise)

Preceding gene: 29348332

Following gene: 29348330

Centisome position: 58.61

GC content: 41.38

Gene sequence:

>1815_bases
ATGATCAAACATTTAACACTTTTGGGTACAGCTCTTTTATTCTCTTCTCAAATTTTATTGGCGCAGTGGAAACCTGCGGG
AGATAAAATCAGAACTTTTTGGGCAGAAAAGGTCGATGTGAATAATGTATTGCCGGAATATCCGCGTCCTATTATGGAAC
GTTCGGATTGGCAGAATCTGAATGGGCTATGGAACTATGCCGTATTACCTTTAGGACAATCTGCTCCGACTACGTTTGAT
GGAAAGATATTAGTTCCTTTTGCTATTGAATCAAGTCTTTCCGGTGTAGGAAAAACTTTGGGGATGGAAAAAGAATTATG
GTACCAACGTGCATTTAATATTCCTTCCAGTTGGAGAGGCAAGAGAGTATTGCTCCATTTTGGCGCAGTAGATTGGAAAA
CTGAAGTGTGGGTAAACAATATAAAAGTAGGTGAACATACCGGTGGTTTTACTCCGTTTACTTTTGATGTGACTGAAGCT
TTGGAGAAATCAACGAATACCCTGACTGTTAAAGTCTGGGACCCTACAGATAAAGGATTTCAACCTCGGGGAAAGCAAGT
TAGTAACCCTCGTGGCATTTGGTATACTCCGGTAAGCGGTATATGGCAGACTGTGTGGCTGGAACCGGTGTCCGAGCATT
ATATTGCAGGTATAAAGACAACTCCCGACATAGATACAAATAAAATAAAGGTAAAAGTTGAAACGGATAGCAATAGACAG
TCGGATAGGCTGGAGGTAAAAGTGTTTGATGGAAAGCATCTGGTGGCTATGGGAACCTCTATAAATGGTTTGCCGGTTGA
GATTCCCATGCCTGCTGATGCAAAGCTCTGGTCGCCGGATAGTCCTTTCCTGTATCAGATGGAAGTATCTTTGATTTCTG
CGGGGAAACTGGTGGATCAGATAAAAAGCTATGTTGCTATGCGCAAGTATTCGATAAAAAGAGATGAACATGGTATTGTG
CGTTTACAGTTGAATAATAAAGACTTGTTCCAGTTTGGTCCTTTAGATCAGGGATGGTGGCCGGACGGATTATATACGGC
TCCTACAGATGAAGCTCTACGCTATGACATTGAGAAAACTAAGGATTTTGGTTTCAACATGATTCGTAAACATATCAAAG
TAGAACCTGCACGTTGGTATACTTACTGTGACCAGATAGGGCTTATCGTATGGCAGGATATGCCTAGTGGAGATAAAAGC
CCGGAGTGGCAAAACCGAAAATATTTTGAGGGAACGGAGTTGACTCGTTCTGCTGAATCGGAAGAGACGTATCGTAAAGA
ATGGAAGGAAGTGATTGATTGTCTTTATTCTTATCCTTGTATTGGTACTTGGGTACCATTCAATGAAGCATGGGGACAAT
TCAAGACACAGGAAATTGCCGAATGGACAAAGCAATATGACCCTTCCCGATTGGTAAATCCGGCAAGTGGAGGGAATCAT
TATACATGTGGTGATATGCTTGATTTACATCACTATCCGGGACCGGAGATGTTTCTGTATGATGCGCAAAGGGCTACAGT
TTTAGGCGAATACGGAGGTATCGGACTGGTGTTGAAAGATCATCTCTGGGAGCCGAATCGTAATTGGGGATATATCCAGT
TCAATACTTCCGCAGAAGCTACAGCAGAATATCTGAAATATGCCGGAATACTGAAAGATATGATTTCCCGTGGATTTTCT
GCAGCTGTCTATACACAAACTACTGATGTTGAAGTCGAAGTAAATGGTTTAATGACTTATGACCGTAAAGTGATAAAACT
CGATGAGTCAAAATTGAAGAAGATGAACACAGAGATTTGCAATTCTTTAAAATAA

Upstream 100 bases:

>100_bases
GACATTATTGCGATTTATTGGGATATTTATATTACCCAAAAACTATCTCATCCGCTAATTTTGTCTGCGTCAATCAGTCG
AAACAATAATCTAATTATAT

Downstream 100 bases:

>100_bases
CAACTAAATAGCAAAACTATATATTATACTTATGAGAAAGACACTAATTTTGATTTTAACCTTGTTTGCAGTCTTTACGA
CTGTTGATGCTAGAAGTAAG

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MIKHLTLLGTALLFSSQILLAQWKPAGDKIRTFWAEKVDVNNVLPEYPRPIMERSDWQNLNGLWNYAVLPLGQSAPTTFD
GKILVPFAIESSLSGVGKTLGMEKELWYQRAFNIPSSWRGKRVLLHFGAVDWKTEVWVNNIKVGEHTGGFTPFTFDVTEA
LEKSTNTLTVKVWDPTDKGFQPRGKQVSNPRGIWYTPVSGIWQTVWLEPVSEHYIAGIKTTPDIDTNKIKVKVETDSNRQ
SDRLEVKVFDGKHLVAMGTSINGLPVEIPMPADAKLWSPDSPFLYQMEVSLISAGKLVDQIKSYVAMRKYSIKRDEHGIV
RLQLNNKDLFQFGPLDQGWWPDGLYTAPTDEALRYDIEKTKDFGFNMIRKHIKVEPARWYTYCDQIGLIVWQDMPSGDKS
PEWQNRKYFEGTELTRSAESEETYRKEWKEVIDCLYSYPCIGTWVPFNEAWGQFKTQEIAEWTKQYDPSRLVNPASGGNH
YTCGDMLDLHHYPGPEMFLYDAQRATVLGEYGGIGLVLKDHLWEPNRNWGYIQFNTSAEATAEYLKYAGILKDMISRGFS
AAVYTQTTDVEVEVNGLMTYDRKVIKLDESKLKKMNTEICNSLK

Sequences:

>Translated_604_residues
MIKHLTLLGTALLFSSQILLAQWKPAGDKIRTFWAEKVDVNNVLPEYPRPIMERSDWQNLNGLWNYAVLPLGQSAPTTFD
GKILVPFAIESSLSGVGKTLGMEKELWYQRAFNIPSSWRGKRVLLHFGAVDWKTEVWVNNIKVGEHTGGFTPFTFDVTEA
LEKSTNTLTVKVWDPTDKGFQPRGKQVSNPRGIWYTPVSGIWQTVWLEPVSEHYIAGIKTTPDIDTNKIKVKVETDSNRQ
SDRLEVKVFDGKHLVAMGTSINGLPVEIPMPADAKLWSPDSPFLYQMEVSLISAGKLVDQIKSYVAMRKYSIKRDEHGIV
RLQLNNKDLFQFGPLDQGWWPDGLYTAPTDEALRYDIEKTKDFGFNMIRKHIKVEPARWYTYCDQIGLIVWQDMPSGDKS
PEWQNRKYFEGTELTRSAESEETYRKEWKEVIDCLYSYPCIGTWVPFNEAWGQFKTQEIAEWTKQYDPSRLVNPASGGNH
YTCGDMLDLHHYPGPEMFLYDAQRATVLGEYGGIGLVLKDHLWEPNRNWGYIQFNTSAEATAEYLKYAGILKDMISRGFS
AAVYTQTTDVEVEVNGLMTYDRKVIKLDESKLKKMNTEICNSLK
>Mature_604_residues
MIKHLTLLGTALLFSSQILLAQWKPAGDKIRTFWAEKVDVNNVLPEYPRPIMERSDWQNLNGLWNYAVLPLGQSAPTTFD
GKILVPFAIESSLSGVGKTLGMEKELWYQRAFNIPSSWRGKRVLLHFGAVDWKTEVWVNNIKVGEHTGGFTPFTFDVTEA
LEKSTNTLTVKVWDPTDKGFQPRGKQVSNPRGIWYTPVSGIWQTVWLEPVSEHYIAGIKTTPDIDTNKIKVKVETDSNRQ
SDRLEVKVFDGKHLVAMGTSINGLPVEIPMPADAKLWSPDSPFLYQMEVSLISAGKLVDQIKSYVAMRKYSIKRDEHGIV
RLQLNNKDLFQFGPLDQGWWPDGLYTAPTDEALRYDIEKTKDFGFNMIRKHIKVEPARWYTYCDQIGLIVWQDMPSGDKS
PEWQNRKYFEGTELTRSAESEETYRKEWKEVIDCLYSYPCIGTWVPFNEAWGQFKTQEIAEWTKQYDPSRLVNPASGGNH
YTCGDMLDLHHYPGPEMFLYDAQRATVLGEYGGIGLVLKDHLWEPNRNWGYIQFNTSAEATAEYLKYAGILKDMISRGFS
AAVYTQTTDVEVEVNGLMTYDRKVIKLDESKLKKMNTEICNSLK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=452, Percent_Identity=23.8938053097345, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1787903, Length=222, Percent_Identity=27.9279279279279, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI48994920, Length=382, Percent_Identity=22.7748691099476, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17510825, Length=229, Percent_Identity=27.9475982532751, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24655438, Length=323, Percent_Identity=24.1486068111455, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19922582, Length=323, Percent_Identity=24.1486068111455, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI62471735, Length=323, Percent_Identity=24.1486068111455, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 69175; Mature: 69175

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKHLTLLGTALLFSSQILLAQWKPAGDKIRTFWAEKVDVNNVLPEYPRPIMERSDWQNL
CCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCC
NGLWNYAVLPLGQSAPTTFDGKILVPFAIESSLSGVGKTLGMEKELWYQRAFNIPSSWRG
CCCCCEEEEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
KRVLLHFGAVDWKTEVWVNNIKVGEHTGGFTPFTFDVTEALEKSTNTLTVKVWDPTDKGF
CEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEEEHHHHHHHCCCCEEEEEEECCCCCCC
QPRGKQVSNPRGIWYTPVSGIWQTVWLEPVSEHYIAGIKTTPDIDTNKIKVKVETDSNRQ
CCCCCCCCCCCCEEECCHHHHHHHHHHCCCHHHHEECCCCCCCCCCCEEEEEEECCCCCC
SDRLEVKVFDGKHLVAMGTSINGLPVEIPMPADAKLWSPDSPFLYQMEVSLISAGKLVDQ
CCEEEEEEECCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHH
IKSYVAMRKYSIKRDEHGIVRLQLNNKDLFQFGPLDQGWWPDGLYTAPTDEALRYDIEKT
HHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEECCCHHHHHCCHHHH
KDFGFNMIRKHIKVEPARWYTYCDQIGLIVWQDMPSGDKSPEWQNRKYFEGTELTRSAES
HCCCHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEECCCHHHCCCCH
EETYRKEWKEVIDCLYSYPCIGTWVPFNEAWGQFKTQEIAEWTKQYDPSRLVNPASGGNH
HHHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCE
YTCGDMLDLHHYPGPEMFLYDAQRATVLGEYGGIGLVLKDHLWEPNRNWGYIQFNTSAEA
EECCCCHHHCCCCCCCEEEEECCCEEEEECCCCEEEEEECCCCCCCCCEEEEEECCCHHH
TAEYLKYAGILKDMISRGFSAAVYTQTTDVEVEVNGLMTYDRKVIKLDESKLKKMNTEIC
HHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCEEEECCEEEEECHHHHHHHHHHHH
NSLK
HCCC
>Mature Secondary Structure
MIKHLTLLGTALLFSSQILLAQWKPAGDKIRTFWAEKVDVNNVLPEYPRPIMERSDWQNL
CCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCC
NGLWNYAVLPLGQSAPTTFDGKILVPFAIESSLSGVGKTLGMEKELWYQRAFNIPSSWRG
CCCCCEEEEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
KRVLLHFGAVDWKTEVWVNNIKVGEHTGGFTPFTFDVTEALEKSTNTLTVKVWDPTDKGF
CEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEEEHHHHHHHCCCCEEEEEEECCCCCCC
QPRGKQVSNPRGIWYTPVSGIWQTVWLEPVSEHYIAGIKTTPDIDTNKIKVKVETDSNRQ
CCCCCCCCCCCCEEECCHHHHHHHHHHCCCHHHHEECCCCCCCCCCCEEEEEEECCCCCC
SDRLEVKVFDGKHLVAMGTSINGLPVEIPMPADAKLWSPDSPFLYQMEVSLISAGKLVDQ
CCEEEEEEECCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHH
IKSYVAMRKYSIKRDEHGIVRLQLNNKDLFQFGPLDQGWWPDGLYTAPTDEALRYDIEKT
HHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCEECCCHHHHHCCHHHH
KDFGFNMIRKHIKVEPARWYTYCDQIGLIVWQDMPSGDKSPEWQNRKYFEGTELTRSAES
HCCCHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEECCCHHHCCCCH
EETYRKEWKEVIDCLYSYPCIGTWVPFNEAWGQFKTQEIAEWTKQYDPSRLVNPASGGNH
HHHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCE
YTCGDMLDLHHYPGPEMFLYDAQRATVLGEYGGIGLVLKDHLWEPNRNWGYIQFNTSAEA
EECCCCHHHCCCCCCCEEEEECCCEEEEECCCCEEEEEECCCCCCCCCEEEEEECCCHHH
TAEYLKYAGILKDMISRGFSAAVYTQTTDVEVEVNGLMTYDRKVIKLDESKLKKMNTEIC
HHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCEEEECCEEEEECHHHHHHHHHHHH
NSLK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA