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The map label for this gene is lacA [C]

Identifier: 29348286

GI number: 29348286

Start: 3596904

End: 3597509

Strand: Reverse

Name: lacA [C]

Synonym: BT_2877

Alternate gene names: 29348286

Gene position: 3597509-3596904 (Counterclockwise)

Preceding gene: 29348287

Following gene: 29348285

Centisome position: 57.46

GC content: 35.97

Gene sequence:

>606_bases
ATGATATTCGGTTTTATTGCAAAAATATATAGGAAATATCAAGAATATATTCATCCTGGTATTTATGTTACGAGGCATGG
TATTCTGTACAGAGAAAAAGATTGCCGATATAATGTATATCCGTTACAAAGATTAATTGTTGGTAAGGTATGGGTTGGAA
ACATTCCTTCACAGGAGAAAGGGCGGCTTATTCTTCATGCTAACTCGGAATTGATAGTAAAAGGGAATTTTGATATTATC
GGTTCAACTGTAGTCGTATTGCCGGATGCTAAACTGATTTTAGGTTCCGGGTATATTAATTTCCATTCGAAGTTACATTG
TTTTAATCACATAGAAATAGGAGAAAATGTTATCATTTCAGAGAATGTAATTATCCGGGATTCTGATAATCACCAGATAA
CGGGAGGAAATAGCATGTTTGCTCCTGTTATAATAAAGGATAATGCATGGATTGGTATGTCTGCTATCATTTTAAAAGGG
GTGACGGTTGGTGAAGGTGCTATTGTTGCAGCAGGTTCTGTCGTGACTAAAGACGTACCGCCGCATACTATTGTCGCAGG
AGTACCGGCAAGGGTAATTAAAAAAGATGTTTATTATACAATATGA

Upstream 100 bases:

>100_bases
GTTTATCTATTTATATATTACTATTGAAAATAAAGCGTGATGATATGTATCTGTTAATAGAGCATATGCTGTTAAGTTAT
TGTAAAAGAAAGATGGCGAG

Downstream 100 bases:

>100_bases
TTATTCAATCTTGTGAGAAACGAAACATTAGTATCTATCATCGTACCTGTCTATAATTCTGCGCAATATTTAAGGAAGTG
CATAGATAGTATTTTGGCAC

Product: putative acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIFGFIAKIYRKYQEYIHPGIYVTRHGILYREKDCRYNVYPLQRLIVGKVWVGNIPSQEKGRLILHANSELIVKGNFDII
GSTVVVLPDAKLILGSGYINFHSKLHCFNHIEIGENVIISENVIIRDSDNHQITGGNSMFAPVIIKDNAWIGMSAIILKG
VTVGEGAIVAAGSVVTKDVPPHTIVAGVPARVIKKDVYYTI

Sequences:

>Translated_201_residues
MIFGFIAKIYRKYQEYIHPGIYVTRHGILYREKDCRYNVYPLQRLIVGKVWVGNIPSQEKGRLILHANSELIVKGNFDII
GSTVVVLPDAKLILGSGYINFHSKLHCFNHIEIGENVIISENVIIRDSDNHQITGGNSMFAPVIIKDNAWIGMSAIILKG
VTVGEGAIVAAGSVVTKDVPPHTIVAGVPARVIKKDVYYTI
>Mature_201_residues
MIFGFIAKIYRKYQEYIHPGIYVTRHGILYREKDCRYNVYPLQRLIVGKVWVGNIPSQEKGRLILHANSELIVKGNFDII
GSTVVVLPDAKLILGSGYINFHSKLHCFNHIEIGENVIISENVIIRDSDNHQITGGNSMFAPVIIKDNAWIGMSAIILKG
VTVGEGAIVAAGSVVTKDVPPHTIVAGVPARVIKKDVYYTI

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=137, Percent_Identity=34.3065693430657, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786664, Length=110, Percent_Identity=39.0909090909091, Blast_Score=70, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322243, Length=87, Percent_Identity=41.3793103448276, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22281; Mature: 22281

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFGFIAKIYRKYQEYIHPGIYVTRHGILYREKDCRYNVYPLQRLIVGKVWVGNIPSQEK
CHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCEECCEEHHHEEEEEEEECCCCCCCC
GRLILHANSELIVKGNFDIIGSTVVVLPDAKLILGSGYINFHSKLHCFNHIEIGENVIIS
CEEEEEECCEEEEEECCEEECCEEEEECCCEEEEECCEEEECCEEEEEEEEECCCEEEEE
ENVIIRDSDNHQITGGNSMFAPVIIKDNAWIGMSAIILKGVTVGEGAIVAAGSVVTKDVP
CCEEEEECCCCEEECCCCEEEEEEEECCCCCCCEEEEEECEEECCCEEEEECCEEECCCC
PHTIVAGVPARVIKKDVYYTI
CCEEEECCCHHHHHCCCEEEC
>Mature Secondary Structure
MIFGFIAKIYRKYQEYIHPGIYVTRHGILYREKDCRYNVYPLQRLIVGKVWVGNIPSQEK
CHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCEECCEEHHHEEEEEEEECCCCCCCC
GRLILHANSELIVKGNFDIIGSTVVVLPDAKLILGSGYINFHSKLHCFNHIEIGENVIIS
CEEEEEECCEEEEEECCEEECCEEEEECCCEEEEECCEEEECCEEEEEEEEECCCEEEEE
ENVIIRDSDNHQITGGNSMFAPVIIKDNAWIGMSAIILKGVTVGEGAIVAAGSVVTKDVP
CCEEEEECCCCEEECCCCEEEEEEEECCCCCCCEEEEEECEEECCCEEEEECCEEECCCC
PHTIVAGVPARVIKKDVYYTI
CCEEEECCCHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]