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The map label for this gene is wcaE [H]

Identifier: 29348283

GI number: 29348283

Start: 3594515

End: 3595315

Strand: Reverse

Name: wcaE [H]

Synonym: BT_2874

Alternate gene names: 29348283

Gene position: 3595315-3594515 (Counterclockwise)

Preceding gene: 29348284

Following gene: 29348282

Centisome position: 57.43

GC content: 34.58

Gene sequence:

>801_bases
ATGAAGATATCAGTTATAACGATTAATTATAATAATGGTGAGCAACTGGAAGCTACCATTCAGTCAGTCGTATCTAATGT
GACAGTATTAAGAGAGCTTTTGGGAGAGAAGGCTGAATACATTGTGATTGATGGTGGAAGCGTTGACTGTAGTGTTTCTG
TGCTTCAAAAATATTCTGAATATATTTCTTATTGGATTAGTGAGAAAGATAAAGGTATTTATCATGCAATGAATAAAGGA
ATTTCTGTTGCACAGGGGGAGTATTGTTTTTTCCTTAATTCAGGTGATACCTTTTATGAAGATGATACACTGAAAAAAGC
TCTTCTTTTCTTAAAGGAGGATTTTGTATGTGGAAATGCTGTTTTAAAATATGCGGGTGGTATAAATGAATGGAGTGCCC
CAGAAATAGTAAATACATTGTTTTTTATGCAGCGTTTTTCCGTGTGTCATCAGTCTTTGTTTATTCGTACGGAACTACTT
AAATCACATCCTTATAATGAAACATTAATGATAGTGGCCGACTATGAACAGATGTTTTATGAAATTGCTGTGAATCACAG
GAGTTATAAAAAAATAGATTTGACTATCTGTTATTATGGATGTGATGGAGTCTCTTCTGATCACGAGAAAGCGGATGCAG
AAAAAAGAAGTGTAATAAATGAGTTTCGGTATTTGGGATATATAGAACCGGACGAACTATGGAACGTGGTGAGTAAACTA
AAAATTGGCACCCGGAAGTATCGGCTTTTATTATCTTTGGCAAAATGTCTGACAAGTAGTCCTAAATATTGGGTGAGATA
A

Upstream 100 bases:

>100_bases
CAGACGGAGTGAAAATTGGAGCGGGAGCAGTTGTTACGAAATCGGTATTAGTTCCGGGAGTGACGGTAGTAGGGGTTCCG
GCTCGTATTGTAGAGAAGTA

Downstream 100 bases:

>100_bases
ATATATTTGTATGAAAAAGACCTCTGTTCCATTGGTAATTGCTTTTACTCCTGATTATTTTATTCCGGCAGCTACATGTC
TCTACTCCATAATTACGAGT

Product: putative colanic acid biosynthesis glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKISVITINYNNGEQLEATIQSVVSNVTVLRELLGEKAEYIVIDGGSVDCSVSVLQKYSEYISYWISEKDKGIYHAMNKG
ISVAQGEYCFFLNSGDTFYEDDTLKKALLFLKEDFVCGNAVLKYAGGINEWSAPEIVNTLFFMQRFSVCHQSLFIRTELL
KSHPYNETLMIVADYEQMFYEIAVNHRSYKKIDLTICYYGCDGVSSDHEKADAEKRSVINEFRYLGYIEPDELWNVVSKL
KIGTRKYRLLLSLAKCLTSSPKYWVR

Sequences:

>Translated_266_residues
MKISVITINYNNGEQLEATIQSVVSNVTVLRELLGEKAEYIVIDGGSVDCSVSVLQKYSEYISYWISEKDKGIYHAMNKG
ISVAQGEYCFFLNSGDTFYEDDTLKKALLFLKEDFVCGNAVLKYAGGINEWSAPEIVNTLFFMQRFSVCHQSLFIRTELL
KSHPYNETLMIVADYEQMFYEIAVNHRSYKKIDLTICYYGCDGVSSDHEKADAEKRSVINEFRYLGYIEPDELWNVVSKL
KIGTRKYRLLLSLAKCLTSSPKYWVR
>Mature_266_residues
MKISVITINYNNGEQLEATIQSVVSNVTVLRELLGEKAEYIVIDGGSVDCSVSVLQKYSEYISYWISEKDKGIYHAMNKG
ISVAQGEYCFFLNSGDTFYEDDTLKKALLFLKEDFVCGNAVLKYAGGINEWSAPEIVNTLFFMQRFSVCHQSLFIRTELL
KSHPYNETLMIVADYEQMFYEIAVNHRSYKKIDLTICYYGCDGVSSDHEKADAEKRSVINEFRYLGYIEPDELWNVVSKL
KIGTRKYRLLLSLAKCLTSSPKYWVR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788368, Length=223, Percent_Identity=29.1479820627803, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 30560; Mature: 30560

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISVITINYNNGEQLEATIQSVVSNVTVLRELLGEKAEYIVIDGGSVDCSVSVLQKYSE
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
YISYWISEKDKGIYHAMNKGISVAQGEYCFFLNSGDTFYEDDTLKKALLFLKEDFVCGNA
HHHHHHCCCCCHHHHHHHCCCEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHH
VLKYAGGINEWSAPEIVNTLFFMQRFSVCHQSLFIRTELLKSHPYNETLMIVADYEQMFY
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHH
EIAVNHRSYKKIDLTICYYGCDGVSSDHEKADAEKRSVINEFRYLGYIEPDELWNVVSKL
HHHHCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KIGTRKYRLLLSLAKCLTSSPKYWVR
HCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKISVITINYNNGEQLEATIQSVVSNVTVLRELLGEKAEYIVIDGGSVDCSVSVLQKYSE
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
YISYWISEKDKGIYHAMNKGISVAQGEYCFFLNSGDTFYEDDTLKKALLFLKEDFVCGNA
HHHHHHCCCCCHHHHHHHCCCEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHH
VLKYAGGINEWSAPEIVNTLFFMQRFSVCHQSLFIRTELLKSHPYNETLMIVADYEQMFY
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHH
EIAVNHRSYKKIDLTICYYGCDGVSSDHEKADAEKRSVINEFRYLGYIEPDELWNVVSKL
HHHHCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KIGTRKYRLLLSLAKCLTSSPKYWVR
HCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]