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The map label for this gene is pckA

Identifier: 29348199

GI number: 29348199

Start: 3459741

End: 3461348

Strand: Reverse

Name: pckA

Synonym: BT_2790

Alternate gene names: 29348199

Gene position: 3461348-3459741 (Counterclockwise)

Preceding gene: 29348201

Following gene: 29348198

Centisome position: 55.29

GC content: 42.85

Gene sequence:

>1608_bases
ATGGCAAATTTAGATTTAAGCAAGTACGGTATCACAGGTGTGACCGAGATTGTGCACAATCCGTCTTATGACGTTTTGTT
CGCTGAAGAAACAAAACCAGGTTTGGAAGGTTTTGAAAAAGGCCAGGTAACTAACATGGGCGCTGTAAACGTAATGACTG
GCGTTTATACAGGACGTTCTCCTAAAGATAAATTCTTCGTAAAAGACGAAACCAGCGAAAATACAGTATGGTGGACTTCT
GAAGAATACAAAAACGACAACAAACCAGTTGACGCTAAATGTTGGGCAGCTGTGAAAGATTTGGCAACTAAAGAACTTTC
TAACAAAAGACTGTTCGTTGTTGACGCTTTCTGCGGAGCTAACGAAAACTCTCGCTTGAAACTGCGCTTCATCATGGAAG
TGGCATGGCAAGCTCACTTCGTAACAAACATGTTCATCCGTCCGACAGCTGAAGAACTGGCTAACTTCGGTGAACCTGAT
TTCGTTATCATGAACGCTTCTAAAGCTAAAGTTGAAAACTACAAAGAACTGGGCTTGAACTCGGAAACTGCAGTTGTATT
CAACTTGACTGAAAAGATCCAGGTTATCCTGAATACTTGGTACGGTGGTGAAATGAAGAAAGGTATGTTCTCTTACATGA
ACTACTTATTGCCTTTGAACGGTATGGCTTCTATGCACTGCTCTGCTAACACTGATAAAGAAGGCAAGAGCTCAGCTATC
TTCTTCGGTCTGTCAGGTACAGGTAAGACTACTTTGTCTACTGACCCGAAACGTTTGTTGATCGGTGATGACGAACACGG
ATGGGATGACGAAGGTGTATTCAACTTCGAAGGTGGTTGCTACGCTAAAGTTATCAACTTGGATAAAGAAAGCGAACCGG
ACATCTGGAATGCTATCAAACGCGATGCTTTGTTGGAAAACTGTACAGTTAACGCTGAAGGCGAAATCAACTTCGCTGAC
AAGTCAGTAACTGAAAACACTCGTGTTTCTTATCCTATCTATCACATCGAAAACATCGTTAAACCGGTGTCTAAAGGTCC
TCATGCTAAACAAGTTATCTTCTTGTCAGCTGACGCATTCGGTGTATTGCCTCCGGTATCTATCCTGAACGCAGAACAAA
CTAAATATTACTTCTTGTCTGGTTTTACAGCTAAATTGGCTGGTACAGAACGTGGTATCACTGAACCGACTCCGACATTC
TCGGCTTGTTTCGGTGCTGCTTTCTTGTCACTGCACCCAACTAAATACGGTGAAGAACTGGTGAAGAAGATGGAAAAAAC
TGGTGCTAAGGCATACTTGGTTAACACTGGTTGGAATGGTACTGGCAAACGTATCTCTATCAAAGATACTCGTGGTATCA
TCGACGCTATCCTTGACGGTTCTATCGACAAAGCTCCTACTAAGGTTATGCCTTACTTCGACTTTGTTGTTCCTACAGAA
CTTCCGGGTGTTGATCCGAAGATCCTTGACCCACGCGACACTTACGAATGTGCTTGCCAATGGGAAGAAAAAGCAAAAGA
TTTGGCTGGACGTTTCATCAAGAACTTCGCTAAATTTACAGGTAACGAAGCTGGTAAGGCTTTAGTTGCTGCTGGTCCGA
AACTCTAA

Upstream 100 bases:

>100_bases
ACTTTTGTACCCGGATTTACAAGGAGGCACCCAAAGCTCCTCATGTTCAGGTGAAAAGAGATAATAAAACGAAATACCAA
TTTTAATTAATTCAAAAAAA

Downstream 100 bases:

>100_bases
GCCTTTTAGGCAATAGATATGTAATGCAAAATGTGGGCGGTTCCGAAAGGAGCCGCCTTTTTTTGTTTATATGAAGGTGA
TTTTGTATCTTTGTTAATCT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MANLDLSKYGITGVTEIVHNPSYDVLFAEETKPGLEGFEKGQVTNMGAVNVMTGVYTGRSPKDKFFVKDETSENTVWWTS
EEYKNDNKPVDAKCWAAVKDLATKELSNKRLFVVDAFCGANENSRLKLRFIMEVAWQAHFVTNMFIRPTAEELANFGEPD
FVIMNASKAKVENYKELGLNSETAVVFNLTEKIQVILNTWYGGEMKKGMFSYMNYLLPLNGMASMHCSANTDKEGKSSAI
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDDEGVFNFEGGCYAKVINLDKESEPDIWNAIKRDALLENCTVNAEGEINFAD
KSVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAFGVLPPVSILNAEQTKYYFLSGFTAKLAGTERGITEPTPTF
SACFGAAFLSLHPTKYGEELVKKMEKTGAKAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIDKAPTKVMPYFDFVVPTE
LPGVDPKILDPRDTYECACQWEEKAKDLAGRFIKNFAKFTGNEAGKALVAAGPKL

Sequences:

>Translated_535_residues
MANLDLSKYGITGVTEIVHNPSYDVLFAEETKPGLEGFEKGQVTNMGAVNVMTGVYTGRSPKDKFFVKDETSENTVWWTS
EEYKNDNKPVDAKCWAAVKDLATKELSNKRLFVVDAFCGANENSRLKLRFIMEVAWQAHFVTNMFIRPTAEELANFGEPD
FVIMNASKAKVENYKELGLNSETAVVFNLTEKIQVILNTWYGGEMKKGMFSYMNYLLPLNGMASMHCSANTDKEGKSSAI
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDDEGVFNFEGGCYAKVINLDKESEPDIWNAIKRDALLENCTVNAEGEINFAD
KSVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAFGVLPPVSILNAEQTKYYFLSGFTAKLAGTERGITEPTPTF
SACFGAAFLSLHPTKYGEELVKKMEKTGAKAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIDKAPTKVMPYFDFVVPTE
LPGVDPKILDPRDTYECACQWEEKAKDLAGRFIKNFAKFTGNEAGKALVAAGPKL
>Mature_534_residues
ANLDLSKYGITGVTEIVHNPSYDVLFAEETKPGLEGFEKGQVTNMGAVNVMTGVYTGRSPKDKFFVKDETSENTVWWTSE
EYKNDNKPVDAKCWAAVKDLATKELSNKRLFVVDAFCGANENSRLKLRFIMEVAWQAHFVTNMFIRPTAEELANFGEPDF
VIMNASKAKVENYKELGLNSETAVVFNLTEKIQVILNTWYGGEMKKGMFSYMNYLLPLNGMASMHCSANTDKEGKSSAIF
FGLSGTGKTTLSTDPKRLLIGDDEHGWDDEGVFNFEGGCYAKVINLDKESEPDIWNAIKRDALLENCTVNAEGEINFADK
SVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAFGVLPPVSILNAEQTKYYFLSGFTAKLAGTERGITEPTPTFS
ACFGAAFLSLHPTKYGEELVKKMEKTGAKAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIDKAPTKVMPYFDFVVPTEL
PGVDPKILDPRDTYECACQWEEKAKDLAGRFIKNFAKFTGNEAGKALVAAGPKL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=532, Percent_Identity=69.7368421052632, Blast_Score=773, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=531, Percent_Identity=43.6911487758945, Blast_Score=440, Evalue=1e-124,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_BACTN (Q8A414)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_811702.1
- ProteinModelPortal:   Q8A414
- SMR:   Q8A414
- GeneID:   1074029
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_2790
- NMPDR:   fig|226186.1.peg.2789
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- PhylomeDB:   Q8A414
- ProtClustDB:   PRK09344
- BioCyc:   BTHE226186:BT_2790-MONOMER
- BRENDA:   4.1.1.49
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 59164; Mature: 59033

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLDLSKYGITGVTEIVHNPSYDVLFAEETKPGLEGFEKGQVTNMGAVNVMTGVYTGRS
CCCCCCHHCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEECCCC
PKDKFFVKDETSENTVWWTSEEYKNDNKPVDAKCWAAVKDLATKELSNKRLFVVDAFCGA
CCCCEEEECCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCC
NENSRLKLRFIMEVAWQAHFVTNMFIRPTAEELANFGEPDFVIMNASKAKVENYKELGLN
CCCCEEEEEEEHHHHHHHHHHHHEEECCCHHHHHCCCCCCEEEEECCHHHHHHHHHHCCC
SETAVVFNLTEKIQVILNTWYGGEMKKGMFSYMNYLLPLNGMASMHCSANTDKEGKSSAI
CCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEE
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDDEGVFNFEGGCYAKVINLDKESEPDIWNAIK
EEEECCCCCCEECCCCCEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHH
RDALLENCTVNAEGEINFADKSVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAF
HHHHHHCCEECCCCCEEECCCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCC
GVLPPVSILNAEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYGEEL
CCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
VKKMEKTGAKAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIDKAPTKVMPYFDFVVPTE
HHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
LPGVDPKILDPRDTYECACQWEEKAKDLAGRFIKNFAKFTGNEAGKALVAAGPKL
CCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
>Mature Secondary Structure 
ANLDLSKYGITGVTEIVHNPSYDVLFAEETKPGLEGFEKGQVTNMGAVNVMTGVYTGRS
CCCCCHHCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEECCCC
PKDKFFVKDETSENTVWWTSEEYKNDNKPVDAKCWAAVKDLATKELSNKRLFVVDAFCGA
CCCCEEEECCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCC
NENSRLKLRFIMEVAWQAHFVTNMFIRPTAEELANFGEPDFVIMNASKAKVENYKELGLN
CCCCEEEEEEEHHHHHHHHHHHHEEECCCHHHHHCCCCCCEEEEECCHHHHHHHHHHCCC
SETAVVFNLTEKIQVILNTWYGGEMKKGMFSYMNYLLPLNGMASMHCSANTDKEGKSSAI
CCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEE
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDDEGVFNFEGGCYAKVINLDKESEPDIWNAIK
EEEECCCCCCEECCCCCEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHH
RDALLENCTVNAEGEINFADKSVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAF
HHHHHHCCEECCCCCEEECCCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEECCCC
GVLPPVSILNAEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYGEEL
CCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
VKKMEKTGAKAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIDKAPTKVMPYFDFVVPTE
HHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
LPGVDPKILDPRDTYECACQWEEKAKDLAGRFIKNFAKFTGNEAGKALVAAGPKL
CCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928