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The map label for this gene is priA [H]

Identifier: 29348161

GI number: 29348161

Start: 3410820

End: 3413276

Strand: Reverse

Name: priA [H]

Synonym: BT_2752

Alternate gene names: 29348161

Gene position: 3413276-3410820 (Counterclockwise)

Preceding gene: 29348162

Following gene: 29348160

Centisome position: 54.52

GC content: 45.5

Gene sequence:

>2457_bases
ATGAAAAAGTATGTAGATGTCATATTACCATTACCGCTTCCGAAGTACTTTACCTATTCCCTGCCGGATGAATGTGCGGA
AGAGGTTGAGATTGGATGCCGTGTGATAGTTCCTTTCGGACGGAAGAAATTTTATACAGCTATCGTTCGTAATGTTCATT
ATTGTGCTCCGACGGAGTATGAGGTGAAGGATATATCTGCATTGCTGGATGCTTCTCCTATTTTATTGTCCACTCAATTT
AAGTTTTGGGAGTGGCTGGCGGATTACTATCTCTGTACACAAGGAGACGTTTATAAAGCGGCACTTCCTTCTGGTTTGAA
GTTGGAGAGTGAAACGATTGTAGAATATAATCCCGATTTTGAGGCGGATGCACCATTATCGGAAAGAGAGCAGCGTATTC
TTGATTTGCTTTCAGTAGATTCTCAGCAATGTGTCACGAAACTGGAGAAGGAGAGTGGCATGAAAAATATCCTCACAGTC
ATCAAATCTCTGCTGGATAAAGAAGCCATCTTTGTGAAAGAGGAATTGCGCCGTACTTACAAGCCGAAAACAGAGGCGAG
AGTACGATTAGCCGGTTCTGCTGATGAAAAGCGGCTTCATATCTTATTTGATATCCTGTCCCGTGCTCCGAAGCAGTTGG
CGTTGCTGATGAAATATGTCGAGTGTTCGGGAATCCTTGGAAGAGAAACACCGAAAGAAGTTTCGAAAAAAGAGCTGTTG
CAACGTGCAGGGGTGTCTCCTTCTATTCTGAATGGCTTGGTGGAGAAAAAGATATTTGAGATTTACTTTCATGAAGTCGG
CCGGCTGAATCAGGAAATGAAGGAGGTCGTAGAGTTGAATGCCTTGAACGAGTTTCAGCAGCGGGCGCACGATGAGATCG
TGCAATCCTTTCAGGAAAAAAATGTCTGCCTGCTTCATGGAGTTACATCCAGTGGAAAGACAGAAGTATACATTCATCTG
ATAGAAGAAACGATTCGTCAAGGGAAACAAGTATTGTATCTCTTGCCTGAAATAGCATTAACCACTCAAATCACAGAGCG
CCTGCAACGGGTATTCGGTTCTCGTTTGGGGATTTATCATTCCAAGTTTCCGGATGCCGAGAGGGTAGAGATATGGCGGA
AGCAACTGGGAGATAAAGGATATGATATCATCCTTGGAGTCCGTTCTTCCGTATTTCTTCCTTTCCGTGATCTGGGCCTG
GTGATTGTAGATGAAGAGCATGAGAACACCTATAAGCAACAGGACCCTGCTCCTCGCTATCATGCACGGAATGCGGCTAT
TGTACTGGCTGCCATGTATGGAGCAAAAACGCTTCTCGGCACTGCTACCCCCTCCATAGAAACATGGCAGAACGCAAGGA
ATGGAAAATATGGATTTGTACAACTGAAAGAACGATATAAAGAAATTCAGCTGCCGGAGATTATTCCGGTGGATATCAAA
GAACTGCATCGCAAGAGGCGGATGGTCGGACAGTTCTCGCCGTTACTGATACAATATATGAAAGAAGCACTGGAACAGAA
AGAACAAGTCATTCTCTTCCAGAACCGTCGCGGATTTGCACCGATGATAGAATGCCGTACTTGCGGCTGGGTACCGAAAT
GTAAGAACTGCGATGTGAGTCTGACTTATCATAAAGGTATCAACCAACTGACCTGCCATTATTGCGGCTATACCTATCAA
CTGCCGAAGTTCTGTCCCGCCTGTGAAGGAACAGAACTGGTCAATCGTGGCTTTGGTACGGAAAAGATTGAAGATGACAT
CAAAGTCCTTTTCCCGGAAGCGGCTGTTGCCCGAATGGATTTAGACACTACCCGTACCCGCGCTGCCTACGAGAAGATTA
TCGCCGATTTTGAGCAGGGAAAGACGGACATACTGATCGGTACGCAAATGGTATCGAAAGGTCTGGACTTTGACCACGTA
AGTATAGTGGGCATCTTGAACGCGGACACCATGCTCAATTATCCGGATTTCCGTTCTTACGAACGTGCTTTCCAACTGAT
GGCACAAGTAGCAGGACGAGCCGGGCGTAAAAATAAACGCGGGCGGGTGGTACTGCAAACGAAAAGCATTGATCACCCCA
TTATCCATCAGGTTATCGGTAATAACTATGAAGAAATGGTAGACGGTCAACTCGCTGAACGACAAATGTTTCATTATCCC
CCTTACTATCGGTTGGTGTATGTCTATCTGAAAAACCATAATGAAGCATTGCTGGACCAGATGGCTGCGGTCATGGCAGA
GAAACTAAGAACTGTGTTCGGAAACCGGGTATTGGGGCCGGACAAACCTCCCGTTGCTCGTATCCAGACATTATTCATCA
AAAAGATAGTGCTGAAGATCGAACAGAATGCCCCGATGGGACGTGCGCGGGAATTGCTACAACGTATCCAGAAAGAGATG
TTGGAGGATGAACGCTATAAATCCTTGATTGTCTATTATGATGTAGACCCCATGTAG

Upstream 100 bases:

>100_bases
ATCTATTTTATCAGATTCCGGTGTGCAGACTATTTTTCTTATCAGTGTTCCCTCTTCCTCTTTTAAATCCCCATTTTTTT
ATTGTACTTTTGTGCTTGCA

Downstream 100 bases:

>100_bases
CACTCGATACAGACCTAATATTTTTCTTATAATAACCTTTATAAATTAACTAACCATGAAACGACTTACGATTTATTGCT
TAACATGTCTTATTGGTGTG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 818; Mature: 818

Protein sequence:

>818_residues
MKKYVDVILPLPLPKYFTYSLPDECAEEVEIGCRVIVPFGRKKFYTAIVRNVHYCAPTEYEVKDISALLDASPILLSTQF
KFWEWLADYYLCTQGDVYKAALPSGLKLESETIVEYNPDFEADAPLSEREQRILDLLSVDSQQCVTKLEKESGMKNILTV
IKSLLDKEAIFVKEELRRTYKPKTEARVRLAGSADEKRLHILFDILSRAPKQLALLMKYVECSGILGRETPKEVSKKELL
QRAGVSPSILNGLVEKKIFEIYFHEVGRLNQEMKEVVELNALNEFQQRAHDEIVQSFQEKNVCLLHGVTSSGKTEVYIHL
IEETIRQGKQVLYLLPEIALTTQITERLQRVFGSRLGIYHSKFPDAERVEIWRKQLGDKGYDIILGVRSSVFLPFRDLGL
VIVDEEHENTYKQQDPAPRYHARNAAIVLAAMYGAKTLLGTATPSIETWQNARNGKYGFVQLKERYKEIQLPEIIPVDIK
ELHRKRRMVGQFSPLLIQYMKEALEQKEQVILFQNRRGFAPMIECRTCGWVPKCKNCDVSLTYHKGINQLTCHYCGYTYQ
LPKFCPACEGTELVNRGFGTEKIEDDIKVLFPEAAVARMDLDTTRTRAAYEKIIADFEQGKTDILIGTQMVSKGLDFDHV
SIVGILNADTMLNYPDFRSYERAFQLMAQVAGRAGRKNKRGRVVLQTKSIDHPIIHQVIGNNYEEMVDGQLAERQMFHYP
PYYRLVYVYLKNHNEALLDQMAAVMAEKLRTVFGNRVLGPDKPPVARIQTLFIKKIVLKIEQNAPMGRARELLQRIQKEM
LEDERYKSLIVYYDVDPM

Sequences:

>Translated_818_residues
MKKYVDVILPLPLPKYFTYSLPDECAEEVEIGCRVIVPFGRKKFYTAIVRNVHYCAPTEYEVKDISALLDASPILLSTQF
KFWEWLADYYLCTQGDVYKAALPSGLKLESETIVEYNPDFEADAPLSEREQRILDLLSVDSQQCVTKLEKESGMKNILTV
IKSLLDKEAIFVKEELRRTYKPKTEARVRLAGSADEKRLHILFDILSRAPKQLALLMKYVECSGILGRETPKEVSKKELL
QRAGVSPSILNGLVEKKIFEIYFHEVGRLNQEMKEVVELNALNEFQQRAHDEIVQSFQEKNVCLLHGVTSSGKTEVYIHL
IEETIRQGKQVLYLLPEIALTTQITERLQRVFGSRLGIYHSKFPDAERVEIWRKQLGDKGYDIILGVRSSVFLPFRDLGL
VIVDEEHENTYKQQDPAPRYHARNAAIVLAAMYGAKTLLGTATPSIETWQNARNGKYGFVQLKERYKEIQLPEIIPVDIK
ELHRKRRMVGQFSPLLIQYMKEALEQKEQVILFQNRRGFAPMIECRTCGWVPKCKNCDVSLTYHKGINQLTCHYCGYTYQ
LPKFCPACEGTELVNRGFGTEKIEDDIKVLFPEAAVARMDLDTTRTRAAYEKIIADFEQGKTDILIGTQMVSKGLDFDHV
SIVGILNADTMLNYPDFRSYERAFQLMAQVAGRAGRKNKRGRVVLQTKSIDHPIIHQVIGNNYEEMVDGQLAERQMFHYP
PYYRLVYVYLKNHNEALLDQMAAVMAEKLRTVFGNRVLGPDKPPVARIQTLFIKKIVLKIEQNAPMGRARELLQRIQKEM
LEDERYKSLIVYYDVDPM
>Mature_818_residues
MKKYVDVILPLPLPKYFTYSLPDECAEEVEIGCRVIVPFGRKKFYTAIVRNVHYCAPTEYEVKDISALLDASPILLSTQF
KFWEWLADYYLCTQGDVYKAALPSGLKLESETIVEYNPDFEADAPLSEREQRILDLLSVDSQQCVTKLEKESGMKNILTV
IKSLLDKEAIFVKEELRRTYKPKTEARVRLAGSADEKRLHILFDILSRAPKQLALLMKYVECSGILGRETPKEVSKKELL
QRAGVSPSILNGLVEKKIFEIYFHEVGRLNQEMKEVVELNALNEFQQRAHDEIVQSFQEKNVCLLHGVTSSGKTEVYIHL
IEETIRQGKQVLYLLPEIALTTQITERLQRVFGSRLGIYHSKFPDAERVEIWRKQLGDKGYDIILGVRSSVFLPFRDLGL
VIVDEEHENTYKQQDPAPRYHARNAAIVLAAMYGAKTLLGTATPSIETWQNARNGKYGFVQLKERYKEIQLPEIIPVDIK
ELHRKRRMVGQFSPLLIQYMKEALEQKEQVILFQNRRGFAPMIECRTCGWVPKCKNCDVSLTYHKGINQLTCHYCGYTYQ
LPKFCPACEGTELVNRGFGTEKIEDDIKVLFPEAAVARMDLDTTRTRAAYEKIIADFEQGKTDILIGTQMVSKGLDFDHV
SIVGILNADTMLNYPDFRSYERAFQLMAQVAGRAGRKNKRGRVVLQTKSIDHPIIHQVIGNNYEEMVDGQLAERQMFHYP
PYYRLVYVYLKNHNEALLDQMAAVMAEKLRTVFGNRVLGPDKPPVARIQTLFIKKIVLKIEQNAPMGRARELLQRIQKEM
LEDERYKSLIVYYDVDPM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=482, Percent_Identity=39.6265560165975, Blast_Score=358, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 94145; Mature: 94145

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYVDVILPLPLPKYFTYSLPDECAEEVEIGCRVIVPFGRKKFYTAIVRNVHYCAPTEY
CCCCEEEEECCCCCCCEECCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCCC
EVKDISALLDASPILLSTQFKFWEWLADYYLCTQGDVYKAALPSGLKLESETIVEYNPDF
CHHHHHHHHCCCCEEEECHHHHHHHHHHHHEECCCCEEHHHCCCCCEECCCEEEEECCCC
EADAPLSEREQRILDLLSVDSQQCVTKLEKESGMKNILTVIKSLLDKEAIFVKEELRRTY
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPKTEARVRLAGSADEKRLHILFDILSRAPKQLALLMKYVECSGILGRETPKEVSKKELL
CCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QRAGVSPSILNGLVEKKIFEIYFHEVGRLNQEMKEVVELNALNEFQQRAHDEIVQSFQEK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NVCLLHGVTSSGKTEVYIHLIEETIRQGKQVLYLLPEIALTTQITERLQRVFGSRLGIYH
CEEEEEECCCCCCCEEEHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHH
SKFPDAERVEIWRKQLGDKGYDIILGVRSSVFLPFRDLGLVIVDEEHENTYKQQDPAPRY
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCEECCHHHCCEEEEECCCCCHHHCCCCCCCH
HARNAAIVLAAMYGAKTLLGTATPSIETWQNARNGKYGFVQLKERYKEIQLPEIIPVDIK
HCCCHHEEHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCCHH
ELHRKRRMVGQFSPLLIQYMKEALEQKEQVILFQNRRGFAPMIECRTCGWVPKCKNCDVS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHCCCCCCCCCCCCEE
LTYHKGINQLTCHYCGYTYQLPKFCPACEGTELVNRGFGTEKIEDDIKVLFPEAAVARMD
EEECCCCCCEEHHHCCCEECCCCCCCCCCCHHHHHCCCCCHHHHCCHHEECCCHHHHHCC
LDTTRTRAAYEKIIADFEQGKTDILIGTQMVSKGLDFDHVSIVGILNADTMLNYPDFRSY
CCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHH
ERAFQLMAQVAGRAGRKNKRGRVVLQTKSIDHPIIHQVIGNNYEEMVDGQLAERQMFHYP
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHCCHHHHHHHHCCC
PYYRLVYVYLKNHNEALLDQMAAVMAEKLRTVFGNRVLGPDKPPVARIQTLFIKKIVLKI
CHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
EQNAPMGRARELLQRIQKEMLEDERYKSLIVYYDVDPM
HCCCCHHHHHHHHHHHHHHHHCCHHHCEEEEEEECCCC
>Mature Secondary Structure
MKKYVDVILPLPLPKYFTYSLPDECAEEVEIGCRVIVPFGRKKFYTAIVRNVHYCAPTEY
CCCCEEEEECCCCCCCEECCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCCC
EVKDISALLDASPILLSTQFKFWEWLADYYLCTQGDVYKAALPSGLKLESETIVEYNPDF
CHHHHHHHHCCCCEEEECHHHHHHHHHHHHEECCCCEEHHHCCCCCEECCCEEEEECCCC
EADAPLSEREQRILDLLSVDSQQCVTKLEKESGMKNILTVIKSLLDKEAIFVKEELRRTY
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPKTEARVRLAGSADEKRLHILFDILSRAPKQLALLMKYVECSGILGRETPKEVSKKELL
CCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QRAGVSPSILNGLVEKKIFEIYFHEVGRLNQEMKEVVELNALNEFQQRAHDEIVQSFQEK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NVCLLHGVTSSGKTEVYIHLIEETIRQGKQVLYLLPEIALTTQITERLQRVFGSRLGIYH
CEEEEEECCCCCCCEEEHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHH
SKFPDAERVEIWRKQLGDKGYDIILGVRSSVFLPFRDLGLVIVDEEHENTYKQQDPAPRY
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCEECCHHHCCEEEEECCCCCHHHCCCCCCCH
HARNAAIVLAAMYGAKTLLGTATPSIETWQNARNGKYGFVQLKERYKEIQLPEIIPVDIK
HCCCHHEEHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCCHH
ELHRKRRMVGQFSPLLIQYMKEALEQKEQVILFQNRRGFAPMIECRTCGWVPKCKNCDVS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHCCCCCCCCCCCCEE
LTYHKGINQLTCHYCGYTYQLPKFCPACEGTELVNRGFGTEKIEDDIKVLFPEAAVARMD
EEECCCCCCEEHHHCCCEECCCCCCCCCCCHHHHHCCCCCHHHHCCHHEECCCHHHHHCC
LDTTRTRAAYEKIIADFEQGKTDILIGTQMVSKGLDFDHVSIVGILNADTMLNYPDFRSY
CCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHH
ERAFQLMAQVAGRAGRKNKRGRVVLQTKSIDHPIIHQVIGNNYEEMVDGQLAERQMFHYP
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHCCHHHHHHHHCCC
PYYRLVYVYLKNHNEALLDQMAAVMAEKLRTVFGNRVLGPDKPPVARIQTLFIKKIVLKI
CHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
EQNAPMGRARELLQRIQKEMLEDERYKSLIVYYDVDPM
HCCCCHHHHHHHHHHHHHHHHCCHHHCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]