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The map label for this gene is gacS [H]

Identifier: 29347984

GI number: 29347984

Start: 3212942

End: 3214201

Strand: Reverse

Name: gacS [H]

Synonym: BT_2574

Alternate gene names: 29347984

Gene position: 3214201-3212942 (Counterclockwise)

Preceding gene: 29347987

Following gene: 29347982

Centisome position: 51.34

GC content: 40.79

Gene sequence:

>1260_bases
ATGGCATTACAGTTTGTTACAGTTGTTTTATTAGCTGGATTCATTTATCTACTGATTAAGTATCAGTCGGTTAAGAAGAA
AATGATATTGGAAACATCTGTAATGCTGCATGAATCCAAAGAGTTGACTTCCATACTACAGAATATAAACGTTTACTATC
TGTTGATTGACAGAGACCTCATCGTCCACAATACCAATTATTATACACTGAATCATTTGACTCCTGCGCAGGGAGAAGAA
AAAAGAGTCGGAGATTTATTGCATTGCAGAAATGCGATAGTAGCCGGAGAATGCGGGAAACATGAACAGTGTAAGTTGTG
CTGTATACGTACATCTATAAGCAAGGCTTTTTATGAGAAGAGAAGCTTTAAGAAACTGGATGCCTCTATGCAACTTTTAA
GTGAGGATGAAAAGACGGTTATTCCCTGTGATGTTTCTGTTTCGGGCACTTATCTGAAAATAAATGGTGAAGAACGTATG
GTGCTGACAGTCTATGATGTGACGGAACTTCGAAATATGCAGCGCCTGCTGGATGTAGAAAGAGAAAATGCGATTTCTGC
CGACAAACTTAAGTCTGCCTTTATCGCTAATATGAGTCACGAAGTCCGTACTCCGTTGAATGCCATTATCGGATTCTCTG
GGTTGATGGCTACGGCTACCAGCGAGGAGGAAAAGAAGATGTATACCGATATTATCTCTGAAAATAATGAGCGGTTATTG
CGACTGGTAAATGATATCTTCGATCTTTCGCAAATAGATGCAGGCGTGTTGAACTTTGTATATTCGGAGTTTGATGCCAA
TGATCTGCTGAGGGAGCTGGAAGGACTTTTCGGAATGAGGCTGAATGAGAATCCTTTGGTGACACTGGTCTGTGAAGCAC
ATCTGGAACCGATTATGATGCATTCGGAGAAGCAGCGTATTATTCAGGTATTGACGAACTTGCTTCATAATGCGATGAAA
TTTACTAAAACAGGTGAAATCCGTTTCGGATGTCGTATGGAAGGCACGGATGAAGTCTATTTTTATGTATCGGATACCGG
TATCGGCATTCCGAAGGAGGAGCAGGAAAAGATATTCTCCCGTTTTACGAAGCTTGATCGGGAGATGCAGGGAACAGGGC
TTGGACTGACACTTTCGCAAACAATTGTTCATAATCTTGGGGGAAGATTCGGTGTAGAATCGGAAGTCGGTAAGGGTAGT
ACTTTTTTGTTCATCTTGCCTTTGGTTACGAAGAAAGAAATAACAAATGATAAGATATGA

Upstream 100 bases:

>100_bases
GGTAGAATAAATATGCAAATGTAGTATCATTTATTCGGTATATTTTATACTTTTACGAGTCAAAACGATCAGGGCACTAC
AAACAATAGAATATATTATT

Downstream 100 bases:

>100_bases
ATAATCCCACCTTGAATCAGGAAATATTCAAGGTGGGATTATTTATGTAAACAATATGTTTAGCTTTTAAGATTTAGGTG
CACCCGCATCTTTAATTCCG

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MALQFVTVVLLAGFIYLLIKYQSVKKKMILETSVMLHESKELTSILQNINVYYLLIDRDLIVHNTNYYTLNHLTPAQGEE
KRVGDLLHCRNAIVAGECGKHEQCKLCCIRTSISKAFYEKRSFKKLDASMQLLSEDEKTVIPCDVSVSGTYLKINGEERM
VLTVYDVTELRNMQRLLDVERENAISADKLKSAFIANMSHEVRTPLNAIIGFSGLMATATSEEEKKMYTDIISENNERLL
RLVNDIFDLSQIDAGVLNFVYSEFDANDLLRELEGLFGMRLNENPLVTLVCEAHLEPIMMHSEKQRIIQVLTNLLHNAMK
FTKTGEIRFGCRMEGTDEVYFYVSDTGIGIPKEEQEKIFSRFTKLDREMQGTGLGLTLSQTIVHNLGGRFGVESEVGKGS
TFLFILPLVTKKEITNDKI

Sequences:

>Translated_419_residues
MALQFVTVVLLAGFIYLLIKYQSVKKKMILETSVMLHESKELTSILQNINVYYLLIDRDLIVHNTNYYTLNHLTPAQGEE
KRVGDLLHCRNAIVAGECGKHEQCKLCCIRTSISKAFYEKRSFKKLDASMQLLSEDEKTVIPCDVSVSGTYLKINGEERM
VLTVYDVTELRNMQRLLDVERENAISADKLKSAFIANMSHEVRTPLNAIIGFSGLMATATSEEEKKMYTDIISENNERLL
RLVNDIFDLSQIDAGVLNFVYSEFDANDLLRELEGLFGMRLNENPLVTLVCEAHLEPIMMHSEKQRIIQVLTNLLHNAMK
FTKTGEIRFGCRMEGTDEVYFYVSDTGIGIPKEEQEKIFSRFTKLDREMQGTGLGLTLSQTIVHNLGGRFGVESEVGKGS
TFLFILPLVTKKEITNDKI
>Mature_418_residues
ALQFVTVVLLAGFIYLLIKYQSVKKKMILETSVMLHESKELTSILQNINVYYLLIDRDLIVHNTNYYTLNHLTPAQGEEK
RVGDLLHCRNAIVAGECGKHEQCKLCCIRTSISKAFYEKRSFKKLDASMQLLSEDEKTVIPCDVSVSGTYLKINGEERMV
LTVYDVTELRNMQRLLDVERENAISADKLKSAFIANMSHEVRTPLNAIIGFSGLMATATSEEEKKMYTDIISENNERLLR
LVNDIFDLSQIDAGVLNFVYSEFDANDLLRELEGLFGMRLNENPLVTLVCEAHLEPIMMHSEKQRIIQVLTNLLHNAMKF
TKTGEIRFGCRMEGTDEVYFYVSDTGIGIPKEEQEKIFSRFTKLDREMQGTGLGLTLSQTIVHNLGGRFGVESEVGKGST
FLFILPLVTKKEITNDKI

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=245, Percent_Identity=33.469387755102, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788713, Length=281, Percent_Identity=29.5373665480427, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87081816, Length=230, Percent_Identity=36.0869565217391, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI48994928, Length=264, Percent_Identity=31.8181818181818, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI145693157, Length=275, Percent_Identity=31.6363636363636, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=28.6343612334802, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786912, Length=272, Percent_Identity=28.3088235294118, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=29.8642533936652, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788549, Length=219, Percent_Identity=27.8538812785388, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786783, Length=235, Percent_Identity=29.3617021276596, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=26.431718061674, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082128, Length=266, Percent_Identity=27.0676691729323, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790861, Length=226, Percent_Identity=26.9911504424779, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47596; Mature: 47465

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQFVTVVLLAGFIYLLIKYQSVKKKMILETSVMLHESKELTSILQNINVYYLLIDRDL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECEE
IVHNTNYYTLNHLTPAQGEEKRVGDLLHCRNAIVAGECGKHEQCKLCCIRTSISKAFYEK
EEECCCEEEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHEEEHHHHHHHHHHHH
RSFKKLDASMQLLSEDEKTVIPCDVSVSGTYLKINGEERMVLTVYDVTELRNMQRLLDVE
HHHHHHHHHHHHHCCCCCEEEEECCEECCEEEEECCCCEEEEEEECHHHHHHHHHHHHHH
RENAISADKLKSAFIANMSHEVRTPLNAIIGFSGLMATATSEEEKKMYTDIISENNERLL
HHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHEECCCHHHHHHHHHHHCCCHHHHH
RLVNDIFDLSQIDAGVLNFVYSEFDANDLLRELEGLFGMRLNENPLVTLVCEAHLEPIMM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHH
HSEKQRIIQVLTNLLHNAMKFTKTGEIRFGCRMEGTDEVYFYVSDTGIGIPKEEQEKIFS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHH
RFTKLDREMQGTGLGLTLSQTIVHNLGGRFGVESEVGKGSTFLFILPLVTKKEITNDKI
HHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECHHHHCCCCCCC
>Mature Secondary Structure 
ALQFVTVVLLAGFIYLLIKYQSVKKKMILETSVMLHESKELTSILQNINVYYLLIDRDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECEE
IVHNTNYYTLNHLTPAQGEEKRVGDLLHCRNAIVAGECGKHEQCKLCCIRTSISKAFYEK
EEECCCEEEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHEEEHHHHHHHHHHHH
RSFKKLDASMQLLSEDEKTVIPCDVSVSGTYLKINGEERMVLTVYDVTELRNMQRLLDVE
HHHHHHHHHHHHHCCCCCEEEEECCEECCEEEEECCCCEEEEEEECHHHHHHHHHHHHHH
RENAISADKLKSAFIANMSHEVRTPLNAIIGFSGLMATATSEEEKKMYTDIISENNERLL
HHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHEECCCHHHHHHHHHHHCCCHHHHH
RLVNDIFDLSQIDAGVLNFVYSEFDANDLLRELEGLFGMRLNENPLVTLVCEAHLEPIMM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHH
HSEKQRIIQVLTNLLHNAMKFTKTGEIRFGCRMEGTDEVYFYVSDTGIGIPKEEQEKIFS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHH
RFTKLDREMQGTGLGLTLSQTIVHNLGGRFGVESEVGKGSTFLFILPLVTKKEITNDKI
HHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]