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The map label for this gene is pnp [H]

Identifier: 29347973

GI number: 29347973

Start: 3199710

End: 3201836

Strand: Reverse

Name: pnp [H]

Synonym: BT_2563

Alternate gene names: 29347973

Gene position: 3201836-3199710 (Counterclockwise)

Preceding gene: 29347977

Following gene: 29347972

Centisome position: 51.14

GC content: 46.12

Gene sequence:

>2127_bases
ATGATTAACCCAATTGTTAAGACGATCGAGTTACCTGATGGAAGAACCATCACACTCGAGACGGGAAAGTTGGCAAAACA
GGCAGACGGTTCTGTAATGCTACGCATGGGAAACACCATGTTATTAGCTACTGTTTGTGCCGCTAAAGATGCAGTTCCCG
GAACAGATTTCATGCCTTTGCAGGTAGAGTATAAAGAAAAATTTGCGGCATTCGGCCGTTTTCCTGGTGGCTTCACCAAG
AGAGAAGGTAGAGCTTCCGATTATGAGATTTTGACTTGTCGTCTCGTTGACCGCGCTCTCCGCCCACTATTCCCTGATAA
TTATCACGCAGAAGTATATGTAAATATCATCCTTTTCTCTGCTGACGGTGTAGATATGCCGGATGCACTGGCCGGATTGG
CAGCTTCTGCAGCACTCGCTGTTTCAGATATTCCTTTCAACGGACCGATCTCGGAAGTACGTGTAGCACGTATCGACGGT
CAGTTTGTAATCAATCCTACTTTTGACCAACTGGAAAAAGCTGATATGGACCTGATGGTTGCCGCTACTTATGATAACAT
CATGATGGTAGAAGGCGAAATGCACGAAGTATCTGAAGCTGAACTGCTGGAAGCAATGAAGGTAGCTCACGAAGCTATCA
AAGTGCATTGCAAGGCTCAGATGGAGCTGACTGAAGAAGTAGGCAAGACAGTGAAACGTGAATATAACCATGAAGTAAAC
GATGAAGAATTGCGTAAGGCAGTTCGTGAGGCTTGTTATGACAAGGCTTATGCAGTAGCTGCTTCCGGAAATAACAACAA
GCACGAACGTTTCGATGCTTTTGATGCTATCCGCGAAGAGTTCAAGGCTCAGTTCTCTGAAGAAGAACTTGAAGAAAAAG
CTCCGCTGATCGACCGTTACTATCATGATGTAGAAAAAGAAGCGATGCGTCGTTCAATTCTTGACGAAGGCAAGCGCCTG
GATGGTCGTAAGACTACTGAAATCCGCCCGATCTGGTGTGAGGTTGGTCCTCTGCCCGGTCCTCACGGATCTGCTATTTT
CACTCGTGGTGAAACTCAGTCTCTGACTTCTGTCACTTTGGGTACGAAGCTCGACGAAAAGATTATCGACAATGTATTGG
AACACGGAAAAGAACGTTTCCTGCTGCACTATAACTTCCCTCCTTTCTCTACAGGCGAAGCAAAAGCTCAACGTGGTGTA
GGCCGTCGTGAAATCGGTCACGGTCACTTGGCTTGGCGTGCCTTGAAAGGACAGATTCCTGCTGACTATCCATACGTAGT
ACGTGTTGTTTCGGAAATCCTCGAATCTAACGGTTCTTCTTCTATGGCTACCGTATGTGCTGGAACATTGGCATTGATGG
ATGCCGGTGTGAAGATCAAGAAGCCGGTATCGGGTATTGCTATGGGACTGATCAAGAATCCGGGCGAAGAAAAATATGCT
GTATTGTCTGACATCCTCGGTGACGAAGACCACCTCGGAGATATGGACTTCAAGGTAACCGGAACAAGAGACGGTATCAC
TGCTACTCAGATGGATATCAAGGTAGACGGTCTGTCTTACGAAATTCTGGAACGTGCTTTGAACCAGGCTAAAGAAGGAC
GTATGCACATCCTCGACAAGATCACCGAGACAATCGCTGAACCGCGTGCTGACCTGAAAGACCATGCTCCTCGCATTGAA
ACAATGACTATTCCTAAAGAATTTATCGGAGCTGTAATCGGCCCTGGTGGAAAAATCATTCAAGGTATGCAGGAAGAGAC
CGGTGCTGTTATCACTATCGAAGAAACAGACGGTATGGGACGCATCGAAGTTTCCGGAACCAACAAGAAATGTATTGATG
ATGCAATGCGCATGATCAAAGCAATCGTTGCTGTTCCTGAAGTAGGCGAAGTATATAAAGGTAAAGTTCGCTCTATCATG
CCTTACGGTGCATTCATTGAATTCCTGCCGGGTAAAGACGGTTTGCTGCACATTTCCGAGATCGACTGGAAACGTCTGGA
AACAGTGGAAGAAGCCGGAATCAAGGAAGGTGATGAGATCGAAGTGAAACTGATCGACATCGATCCGAAGACAGGAAAAT
TCAAACTTTCAAGAAAAGTATTGATGCCAAGACCGGAAAAGAAGTAA

Upstream 100 bases:

>100_bases
ATATAGCGTCTAACTTTTTAGCTCATTTCCACCTGATTACAACGAGAAATGTATATCTTTGCAGGAATTTTGAAAGAAAA
GTATAGATAAAACAATTTTT

Downstream 100 bases:

>100_bases
AAAAGCAGATAGCTTTTAGATAATGTGATAGCCCGATTACTCAGTATTCGGGCTATTTTTTTTGTCTATTATTTTGCTTT
TCTGCGAAAATATGTGTTCT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MINPIVKTIELPDGRTITLETGKLAKQADGSVMLRMGNTMLLATVCAAKDAVPGTDFMPLQVEYKEKFAAFGRFPGGFTK
REGRASDYEILTCRLVDRALRPLFPDNYHAEVYVNIILFSADGVDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDG
QFVINPTFDQLEKADMDLMVAATYDNIMMVEGEMHEVSEAELLEAMKVAHEAIKVHCKAQMELTEEVGKTVKREYNHEVN
DEELRKAVREACYDKAYAVAASGNNNKHERFDAFDAIREEFKAQFSEEELEEKAPLIDRYYHDVEKEAMRRSILDEGKRL
DGRKTTEIRPIWCEVGPLPGPHGSAIFTRGETQSLTSVTLGTKLDEKIIDNVLEHGKERFLLHYNFPPFSTGEAKAQRGV
GRREIGHGHLAWRALKGQIPADYPYVVRVVSEILESNGSSSMATVCAGTLALMDAGVKIKKPVSGIAMGLIKNPGEEKYA
VLSDILGDEDHLGDMDFKVTGTRDGITATQMDIKVDGLSYEILERALNQAKEGRMHILDKITETIAEPRADLKDHAPRIE
TMTIPKEFIGAVIGPGGKIIQGMQEETGAVITIEETDGMGRIEVSGTNKKCIDDAMRMIKAIVAVPEVGEVYKGKVRSIM
PYGAFIEFLPGKDGLLHISEIDWKRLETVEEAGIKEGDEIEVKLIDIDPKTGKFKLSRKVLMPRPEKK

Sequences:

>Translated_708_residues
MINPIVKTIELPDGRTITLETGKLAKQADGSVMLRMGNTMLLATVCAAKDAVPGTDFMPLQVEYKEKFAAFGRFPGGFTK
REGRASDYEILTCRLVDRALRPLFPDNYHAEVYVNIILFSADGVDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDG
QFVINPTFDQLEKADMDLMVAATYDNIMMVEGEMHEVSEAELLEAMKVAHEAIKVHCKAQMELTEEVGKTVKREYNHEVN
DEELRKAVREACYDKAYAVAASGNNNKHERFDAFDAIREEFKAQFSEEELEEKAPLIDRYYHDVEKEAMRRSILDEGKRL
DGRKTTEIRPIWCEVGPLPGPHGSAIFTRGETQSLTSVTLGTKLDEKIIDNVLEHGKERFLLHYNFPPFSTGEAKAQRGV
GRREIGHGHLAWRALKGQIPADYPYVVRVVSEILESNGSSSMATVCAGTLALMDAGVKIKKPVSGIAMGLIKNPGEEKYA
VLSDILGDEDHLGDMDFKVTGTRDGITATQMDIKVDGLSYEILERALNQAKEGRMHILDKITETIAEPRADLKDHAPRIE
TMTIPKEFIGAVIGPGGKIIQGMQEETGAVITIEETDGMGRIEVSGTNKKCIDDAMRMIKAIVAVPEVGEVYKGKVRSIM
PYGAFIEFLPGKDGLLHISEIDWKRLETVEEAGIKEGDEIEVKLIDIDPKTGKFKLSRKVLMPRPEKK
>Mature_708_residues
MINPIVKTIELPDGRTITLETGKLAKQADGSVMLRMGNTMLLATVCAAKDAVPGTDFMPLQVEYKEKFAAFGRFPGGFTK
REGRASDYEILTCRLVDRALRPLFPDNYHAEVYVNIILFSADGVDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDG
QFVINPTFDQLEKADMDLMVAATYDNIMMVEGEMHEVSEAELLEAMKVAHEAIKVHCKAQMELTEEVGKTVKREYNHEVN
DEELRKAVREACYDKAYAVAASGNNNKHERFDAFDAIREEFKAQFSEEELEEKAPLIDRYYHDVEKEAMRRSILDEGKRL
DGRKTTEIRPIWCEVGPLPGPHGSAIFTRGETQSLTSVTLGTKLDEKIIDNVLEHGKERFLLHYNFPPFSTGEAKAQRGV
GRREIGHGHLAWRALKGQIPADYPYVVRVVSEILESNGSSSMATVCAGTLALMDAGVKIKKPVSGIAMGLIKNPGEEKYA
VLSDILGDEDHLGDMDFKVTGTRDGITATQMDIKVDGLSYEILERALNQAKEGRMHILDKITETIAEPRADLKDHAPRIE
TMTIPKEFIGAVIGPGGKIIQGMQEETGAVITIEETDGMGRIEVSGTNKKCIDDAMRMIKAIVAVPEVGEVYKGKVRSIM
PYGAFIEFLPGKDGLLHISEIDWKRLETVEEAGIKEGDEIEVKLIDIDPKTGKFKLSRKVLMPRPEKK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=38.2352941176471, Blast_Score=468, Evalue=1e-132,
Organism=Escherichia coli, GI145693187, Length=706, Percent_Identity=45.6090651558074, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI115534063, Length=708, Percent_Identity=35.1694915254237, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281362905, Length=713, Percent_Identity=37.5876577840112, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=713, Percent_Identity=37.5876577840112, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=713, Percent_Identity=37.5876577840112, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=650, Percent_Identity=37.5384615384615, Blast_Score=409, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78369; Mature: 78369

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPIVKTIELPDGRTITLETGKLAKQADGSVMLRMGNTMLLATVCAAKDAVPGTDFMPL
CCCCCEEEEECCCCCEEEEECCCHHHHCCCCEEEEECCEEEEHHHHHHCCCCCCCCCEEE
QVEYKEKFAAFGRFPGGFTKREGRASDYEILTCRLVDRALRPLFPDNYHAEVYVNIILFS
EEHHHHHHHHHCCCCCCCCCCCCCCCCHHEEHHHHHHHHHHCCCCCCCCEEEEEEEEEEE
ADGVDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDGQFVINPTFDQLEKADMDLMV
CCCCCCCHHHHHHHHHHHHEEECCCCCCCHHHEEEEEECCEEEECCCHHHHHHCCCCEEE
AATYDNIMMVEGEMHEVSEAELLEAMKVAHEAIKVHCKAQMELTEEVGKTVKREYNHEVN
EEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEELRKAVREACYDKAYAVAASGNNNKHERFDAFDAIREEFKAQFSEEELEEKAPLIDRY
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHH
YHDVEKEAMRRSILDEGKRLDGRKTTEIRPIWCEVGPLPGPHGSAIFTRGETQSLTSVTL
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCHHEEEC
GTKLDEKIIDNVLEHGKERFLLHYNFPPFSTGEAKAQRGVGRREIGHGHLAWRALKGQIP
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCHHCCCCHHHHHHHCCCCC
ADYPYVVRVVSEILESNGSSSMATVCAGTLALMDAGVKIKKPVSGIAMGLIKNPGEEKYA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCHHHH
VLSDILGDEDHLGDMDFKVTGTRDGITATQMDIKVDGLSYEILERALNQAKEGRMHILDK
HHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHH
ITETIAEPRADLKDHAPRIETMTIPKEFIGAVIGPGGKIIQGMQEETGAVITIEETDGMG
HHHHHHCCHHHHHHCCCCCEEEECCHHHHHHEECCCHHHHHCHHHCCCCEEEEEECCCCE
RIEVSGTNKKCIDDAMRMIKAIVAVPEVGEVYKGKVRSIMPYGAFIEFLPGKDGLLHISE
EEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHCCCCCHHHEECCCCCCEEEEEC
IDWKRLETVEEAGIKEGDEIEVKLIDIDPKTGKFKLSRKVLMPRPEKK
CCHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEECEEECCCCCCC
>Mature Secondary Structure
MINPIVKTIELPDGRTITLETGKLAKQADGSVMLRMGNTMLLATVCAAKDAVPGTDFMPL
CCCCCEEEEECCCCCEEEEECCCHHHHCCCCEEEEECCEEEEHHHHHHCCCCCCCCCEEE
QVEYKEKFAAFGRFPGGFTKREGRASDYEILTCRLVDRALRPLFPDNYHAEVYVNIILFS
EEHHHHHHHHHCCCCCCCCCCCCCCCCHHEEHHHHHHHHHHCCCCCCCCEEEEEEEEEEE
ADGVDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDGQFVINPTFDQLEKADMDLMV
CCCCCCCHHHHHHHHHHHHEEECCCCCCCHHHEEEEEECCEEEECCCHHHHHHCCCCEEE
AATYDNIMMVEGEMHEVSEAELLEAMKVAHEAIKVHCKAQMELTEEVGKTVKREYNHEVN
EEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEELRKAVREACYDKAYAVAASGNNNKHERFDAFDAIREEFKAQFSEEELEEKAPLIDRY
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHH
YHDVEKEAMRRSILDEGKRLDGRKTTEIRPIWCEVGPLPGPHGSAIFTRGETQSLTSVTL
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCHHEEEC
GTKLDEKIIDNVLEHGKERFLLHYNFPPFSTGEAKAQRGVGRREIGHGHLAWRALKGQIP
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCHHCCCCHHHHHHHCCCCC
ADYPYVVRVVSEILESNGSSSMATVCAGTLALMDAGVKIKKPVSGIAMGLIKNPGEEKYA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCHHHH
VLSDILGDEDHLGDMDFKVTGTRDGITATQMDIKVDGLSYEILERALNQAKEGRMHILDK
HHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHH
ITETIAEPRADLKDHAPRIETMTIPKEFIGAVIGPGGKIIQGMQEETGAVITIEETDGMG
HHHHHHCCHHHHHHCCCCCEEEECCHHHHHHEECCCHHHHHCHHHCCCCEEEEEECCCCE
RIEVSGTNKKCIDDAMRMIKAIVAVPEVGEVYKGKVRSIMPYGAFIEFLPGKDGLLHISE
EEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHCCCCCHHHEECCCCCCEEEEEC
IDWKRLETVEEAGIKEGDEIEVKLIDIDPKTGKFKLSRKVLMPRPEKK
CCHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEECEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA