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The map label for this gene is thrA [H]

Identifier: 29347813

GI number: 29347813

Start: 2999048

End: 3001483

Strand: Reverse

Name: thrA [H]

Synonym: BT_2403

Alternate gene names: 29347813

Gene position: 3001483-2999048 (Counterclockwise)

Preceding gene: 29347820

Following gene: 29347812

Centisome position: 47.94

GC content: 46.96

Gene sequence:

>2436_bases
ATGAAAGTAATGAAATTCGGCGGAACGTCCGTAGGTTCCGTGAACAGCATTTTAAGTGTAAAGAGAATTGTAGAGTCGGC
CGGCGAGCCGGTAATTGTAGTTGTTTCCGCGTTAGGGGGCATCACTGACAAATTGATTAATACATCGAAGATGGCGGCAG
CCGGAGATTCGGCCTACGAAGGAGAGTTCCGCGAAATCGTCTATCGTCATGTGGAGATGATTAAAGAAGTGATACCTGCC
GGAGCAGGGCAAGTGGCATTGCAACGTCAGATTGGCGAATTGCTGAATGAGTTGAAAGACATTTTCCAGGGTATCTATCT
GATCAGAGACCTTTCCCCGAAAACATCGGATACCATTGTCAGCTATGGCGAACGTCTTTCGTCCATCATCGTTGCCGAAC
TGATCGATGAGGCAAAATGGTTTGATTCACGTACTTTTATCAAGACTGAAAAGAAGCATAATAAACATACGATTGATGCC
GATCTGACTAATCAGTTGGTAAAAGAGGCTTTCCATTCCATTCCGAAAGTGTCGTTGGTTCCCGGATTTATTTCTTCGGA
TAAGGTGTCGGGAGACGTAACGAATCTCGGTCGTGGTGGTTCGGATTATACGGCTGCCATTATTGCCGCAGCGCTCGATG
CCAGCAGTCTGGAGATATGGACCGATGTAGACGGCTTTATGACTGCCGATCCGCGTGTGATCTCAACAGCTTATACTATT
TCCGAACTGAGCTATGTAGAAGCAACCGAACTCTGTAACTTCGGTGCGAAAGTTGTTTATCCGCCGACGATCTATCCGGT
ATGTCATAAGAATATTCCTATTATAATAAAGAATACGTTCAATCCTGACGGAGTGGGAACAGTTATCAAGCAAGAGACTT
CCAATCCGCAGAGTAAGGCCATTAAAGGTATTTCCTCTATCAATGATACGAGTCTGATCACTGTTCAGGGGCTGGGTATG
GTAGGTGTGATCGGTGTAAACTACCGTATCTTCAAGGCTTTGGCAAAGAATGGAATCAGTGTATTCCTTGTTTCGCAGGC
TTCATCAGAGAACAGTACCTCTATCGGTGTTCGGAACGCTGATGCCGATCTGGCTTGTGAAGTGCTGAATGAAGAATTTG
CGAAAGAGATCGAGATGGGAGAGATTTCTCCGATCCTTGCAGAGAGAAATCTGGCTACGGTGGCAATCGTTGGCGAGAAT
ATGAAGCATACACCGGGTATAGCAGGGAAGCTCTTCGGTACACTGGGACGTAATGGTATCAATGTAATCGCTTGTGCGCA
GGGAGCTTCGGAAACCAACATTTCTTTTGTTGTCGATTCCAAGTCATTGCGTAAATCTCTGAACGTTATCCATGACTCTT
TCTTCCTGTCCGAATATCAGGTATTGAATCTCTTTATCTGTGGTATCGGTACGGTTGGCGGCAGTCTGGTAGAACAGATT
CGTTGCCAGCAACAGAAACTGATGATGGAGAACGGATTGAAGTTGCACGTCGTAGGTATCATCGATGCTGCGAAAGCCAT
GTTCAGTCGTGAGGGATTTGACCTTGCCAATTTCCGTGAGGAGTTGCAGAAGAAAGGCAAGGATAGTAACTTGCAGACGA
TCCGTGACGAAATAGTCGGAATGAATATCTTCAACTCCGTATTCGTGGATTGTACGGCAAGCCCCGACATTGCATCACTT
TACAAAGACCTTTTACAGCACAACGTTTCCGTAGTGGCAGCCAATAAGATCGCTGCGTCTTCGGCTTACGAGAACTATCG
CGAACTGAAAACGATTGCCCGTCAACGGGGTGTGAAGTACTTGTTTGAAACAAACGTAGGTGCGGGACTGCCGATTATCA
ATACCATCAACGACCTGATTCACAGTGGTGATAAGATACTGAAGATTGAAGCTGTACTTTCGGGTACATTGAACTATATC
TTTAATAAGATCAGCGCTGACATTCCTTTCAGCCGCACGATCAAGATGGCACAGGAAGAACGCTATTCCGAACCGGACCC
GCGTATCGACCTGAGCGGAAAAGACGTGATCCGCAAACTGGTGATTCTGGCACGTGAAGCCGGATATCACATCGAGCAGG
AAGACGTAGAAAAGAATCTCTTTGTCCCGAACGACTTCTTCGAAGGTTCGCTGGATGACTTCTGGAAACGAGTGCCGAGC
CTCGACGCAGACTTCGAAGCCCGTCGTCAGGTATTGGAGAAAGAACATAAACATTGGCGCTTTGTTGCCAAACTGGAAGA
CGGAAAAGCCTCTGTCGGCCTGCAGGAAGTGGGTGCCAACCATCCGTTCTACGGACTGGAAGGCAGCAACAACATCATCT
TGCTGACTACGGAACGCTATAAAGAATATCCGATGATGATTCAAGGATACGGTGCCGGTGCCGGAGTAACGGCTGCGGGA
GTATTCGCTGATATCATGAGTATCGCAAACGTTTAA

Upstream 100 bases:

>100_bases
CCTGTGTAAGTAGGCAAAACTCAAATTTAATTTTTTCGTAAACAACCAATCACCCTGTAATAACTATACTCGAGGGGATT
GTATTTAAATATGTATCTTC

Downstream 100 bases:

>100_bases
ATAAGGGTTATTAAAATGAAGCATATTATCATACTGGGAGATGGAATGGCCGACTGGCCAGTGAAGTCATTGGGGGACAA
GACGCTCCTGCAATATGCGA

Product: bifunctional aspartokinase I/homoserine dehydrogenase I

Products: NA

Alternate protein names: Aspartokinase I/homoserine dehydrogenase I; AKI-HDI; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 811; Mature: 811

Protein sequence:

>811_residues
MKVMKFGGTSVGSVNSILSVKRIVESAGEPVIVVVSALGGITDKLINTSKMAAAGDSAYEGEFREIVYRHVEMIKEVIPA
GAGQVALQRQIGELLNELKDIFQGIYLIRDLSPKTSDTIVSYGERLSSIIVAELIDEAKWFDSRTFIKTEKKHNKHTIDA
DLTNQLVKEAFHSIPKVSLVPGFISSDKVSGDVTNLGRGGSDYTAAIIAAALDASSLEIWTDVDGFMTADPRVISTAYTI
SELSYVEATELCNFGAKVVYPPTIYPVCHKNIPIIIKNTFNPDGVGTVIKQETSNPQSKAIKGISSINDTSLITVQGLGM
VGVIGVNYRIFKALAKNGISVFLVSQASSENSTSIGVRNADADLACEVLNEEFAKEIEMGEISPILAERNLATVAIVGEN
MKHTPGIAGKLFGTLGRNGINVIACAQGASETNISFVVDSKSLRKSLNVIHDSFFLSEYQVLNLFICGIGTVGGSLVEQI
RCQQQKLMMENGLKLHVVGIIDAAKAMFSREGFDLANFREELQKKGKDSNLQTIRDEIVGMNIFNSVFVDCTASPDIASL
YKDLLQHNVSVVAANKIAASSAYENYRELKTIARQRGVKYLFETNVGAGLPIINTINDLIHSGDKILKIEAVLSGTLNYI
FNKISADIPFSRTIKMAQEERYSEPDPRIDLSGKDVIRKLVILAREAGYHIEQEDVEKNLFVPNDFFEGSLDDFWKRVPS
LDADFEARRQVLEKEHKHWRFVAKLEDGKASVGLQEVGANHPFYGLEGSNNIILLTTERYKEYPMMIQGYGAGAGVTAAG
VFADIMSIANV

Sequences:

>Translated_811_residues
MKVMKFGGTSVGSVNSILSVKRIVESAGEPVIVVVSALGGITDKLINTSKMAAAGDSAYEGEFREIVYRHVEMIKEVIPA
GAGQVALQRQIGELLNELKDIFQGIYLIRDLSPKTSDTIVSYGERLSSIIVAELIDEAKWFDSRTFIKTEKKHNKHTIDA
DLTNQLVKEAFHSIPKVSLVPGFISSDKVSGDVTNLGRGGSDYTAAIIAAALDASSLEIWTDVDGFMTADPRVISTAYTI
SELSYVEATELCNFGAKVVYPPTIYPVCHKNIPIIIKNTFNPDGVGTVIKQETSNPQSKAIKGISSINDTSLITVQGLGM
VGVIGVNYRIFKALAKNGISVFLVSQASSENSTSIGVRNADADLACEVLNEEFAKEIEMGEISPILAERNLATVAIVGEN
MKHTPGIAGKLFGTLGRNGINVIACAQGASETNISFVVDSKSLRKSLNVIHDSFFLSEYQVLNLFICGIGTVGGSLVEQI
RCQQQKLMMENGLKLHVVGIIDAAKAMFSREGFDLANFREELQKKGKDSNLQTIRDEIVGMNIFNSVFVDCTASPDIASL
YKDLLQHNVSVVAANKIAASSAYENYRELKTIARQRGVKYLFETNVGAGLPIINTINDLIHSGDKILKIEAVLSGTLNYI
FNKISADIPFSRTIKMAQEERYSEPDPRIDLSGKDVIRKLVILAREAGYHIEQEDVEKNLFVPNDFFEGSLDDFWKRVPS
LDADFEARRQVLEKEHKHWRFVAKLEDGKASVGLQEVGANHPFYGLEGSNNIILLTTERYKEYPMMIQGYGAGAGVTAAG
VFADIMSIANV
>Mature_811_residues
MKVMKFGGTSVGSVNSILSVKRIVESAGEPVIVVVSALGGITDKLINTSKMAAAGDSAYEGEFREIVYRHVEMIKEVIPA
GAGQVALQRQIGELLNELKDIFQGIYLIRDLSPKTSDTIVSYGERLSSIIVAELIDEAKWFDSRTFIKTEKKHNKHTIDA
DLTNQLVKEAFHSIPKVSLVPGFISSDKVSGDVTNLGRGGSDYTAAIIAAALDASSLEIWTDVDGFMTADPRVISTAYTI
SELSYVEATELCNFGAKVVYPPTIYPVCHKNIPIIIKNTFNPDGVGTVIKQETSNPQSKAIKGISSINDTSLITVQGLGM
VGVIGVNYRIFKALAKNGISVFLVSQASSENSTSIGVRNADADLACEVLNEEFAKEIEMGEISPILAERNLATVAIVGEN
MKHTPGIAGKLFGTLGRNGINVIACAQGASETNISFVVDSKSLRKSLNVIHDSFFLSEYQVLNLFICGIGTVGGSLVEQI
RCQQQKLMMENGLKLHVVGIIDAAKAMFSREGFDLANFREELQKKGKDSNLQTIRDEIVGMNIFNSVFVDCTASPDIASL
YKDLLQHNVSVVAANKIAASSAYENYRELKTIARQRGVKYLFETNVGAGLPIINTINDLIHSGDKILKIEAVLSGTLNYI
FNKISADIPFSRTIKMAQEERYSEPDPRIDLSGKDVIRKLVILAREAGYHIEQEDVEKNLFVPNDFFEGSLDDFWKRVPS
LDADFEARRQVLEKEHKHWRFVAKLEDGKASVGLQEVGANHPFYGLEGSNNIILLTTERYKEYPMMIQGYGAGAGVTAAG
VFADIMSIANV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=817, Percent_Identity=35.6181150550796, Blast_Score=477, Evalue=1e-135,
Organism=Escherichia coli, GI1790376, Length=826, Percent_Identity=29.4188861985472, Blast_Score=333, Evalue=4e-92,
Organism=Escherichia coli, GI1790455, Length=462, Percent_Identity=30.0865800865801, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320893, Length=514, Percent_Identity=28.0155642023346, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322599, Length=363, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88525; Mature: 88525

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMKFGGTSVGSVNSILSVKRIVESAGEPVIVVVSALGGITDKLINTSKMAAAGDSAYE
CCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHCCHHHHCCCCCCCC
GEFREIVYRHVEMIKEVIPAGAGQVALQRQIGELLNELKDIFQGIYLIRDLSPKTSDTIV
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SYGERLSSIIVAELIDEAKWFDSRTFIKTEKKHNKHTIDADLTNQLVKEAFHSIPKVSLV
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEECCHHHHHHHHHHHHHCCCCEEEC
PGFISSDKVSGDVTNLGRGGSDYTAAIIAAALDASSLEIWTDVDGFMTADPRVISTAYTI
CCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHH
SELSYVEATELCNFGAKVVYPPTIYPVCHKNIPIIIKNTFNPDGVGTVIKQETSNPQSKA
HHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCEEEECCCCCCCCCHHHCCCCCCCHHHH
IKGISSINDTSLITVQGLGMVGVIGVNYRIFKALAKNGISVFLVSQASSENSTSIGVRNA
HHHHCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECC
DADLACEVLNEEFAKEIEMGEISPILAERNLATVAIVGENMKHTPGIAGKLFGTLGRNGI
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCC
NVIACAQGASETNISFVVDSKSLRKSLNVIHDSFFLSEYQVLNLFICGIGTVGGSLVEQI
EEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RCQQQKLMMENGLKLHVVGIIDAAKAMFSREGFDLANFREELQKKGKDSNLQTIRDEIVG
HHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHC
MNIFNSVFVDCTASPDIASLYKDLLQHNVSVVAANKIAASSAYENYRELKTIARQRGVKY
HHHHHHEEEEECCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEE
LFETNVGAGLPIINTINDLIHSGDKILKIEAVLSGTLNYIFNKISADIPFSRTIKMAQEE
EEECCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RYSEPDPRIDLSGKDVIRKLVILAREAGYHIEQEDVEKNLFVPNDFFEGSLDDFWKRVPS
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHCCC
LDADFEARRQVLEKEHKHWRFVAKLEDGKASVGLQEVGANHPFYGLEGSNNIILLTTERY
CCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHCCCCCEEEECCCCCEEEEECCCC
KEYPMMIQGYGAGAGVTAAGVFADIMSIANV
CCCCCEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVMKFGGTSVGSVNSILSVKRIVESAGEPVIVVVSALGGITDKLINTSKMAAAGDSAYE
CCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHCCHHHHCCCCCCCC
GEFREIVYRHVEMIKEVIPAGAGQVALQRQIGELLNELKDIFQGIYLIRDLSPKTSDTIV
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SYGERLSSIIVAELIDEAKWFDSRTFIKTEKKHNKHTIDADLTNQLVKEAFHSIPKVSLV
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEECCHHHHHHHHHHHHHCCCCEEEC
PGFISSDKVSGDVTNLGRGGSDYTAAIIAAALDASSLEIWTDVDGFMTADPRVISTAYTI
CCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHH
SELSYVEATELCNFGAKVVYPPTIYPVCHKNIPIIIKNTFNPDGVGTVIKQETSNPQSKA
HHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCEEEECCCCCCCCCHHHCCCCCCCHHHH
IKGISSINDTSLITVQGLGMVGVIGVNYRIFKALAKNGISVFLVSQASSENSTSIGVRNA
HHHHCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECC
DADLACEVLNEEFAKEIEMGEISPILAERNLATVAIVGENMKHTPGIAGKLFGTLGRNGI
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCC
NVIACAQGASETNISFVVDSKSLRKSLNVIHDSFFLSEYQVLNLFICGIGTVGGSLVEQI
EEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RCQQQKLMMENGLKLHVVGIIDAAKAMFSREGFDLANFREELQKKGKDSNLQTIRDEIVG
HHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHC
MNIFNSVFVDCTASPDIASLYKDLLQHNVSVVAANKIAASSAYENYRELKTIARQRGVKY
HHHHHHEEEEECCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEE
LFETNVGAGLPIINTINDLIHSGDKILKIEAVLSGTLNYIFNKISADIPFSRTIKMAQEE
EEECCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RYSEPDPRIDLSGKDVIRKLVILAREAGYHIEQEDVEKNLFVPNDFFEGSLDDFWKRVPS
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHCCC
LDADFEARRQVLEKEHKHWRFVAKLEDGKASVGLQEVGANHPFYGLEGSNNIILLTTERY
CCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHCCCCCEEEECCCCCEEEEECCCC
KEYPMMIQGYGAGAGVTAAGVFADIMSIANV
CCCCCEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7003595; 1630901; 7610040; 9278503; 6277952; 2410621; 387092; 6298218; 390305 [H]