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The map label for this gene is btuB [H]

Identifier: 29347475

GI number: 29347475

Start: 2584894

End: 2587284

Strand: Reverse

Name: btuB [H]

Synonym: BT_2065

Alternate gene names: 29347475

Gene position: 2587284-2584894 (Counterclockwise)

Preceding gene: 29347476

Following gene: 29347474

Centisome position: 41.33

GC content: 45.67

Gene sequence:

>2391_bases
ATGATCAATAAAAGTTTTCTGGTTTTTCTTGCGTTCTTATTTTCAGTGTCAGCATTTTCTCAACATGGGCGATCCGGAGG
GATGATTTCCGGAAGAGTGATTTCTACTGTAGAAAAAGAAGTGGTGGACTTTGCGACTGTATATCTGAAAGGGACGAACA
GAGGAGCGACTACAGATGAAAAGGGATTGTATCACTTGAAAGCGGCAGCAGGCGACTACACTCTGGTTGTATCGGCCATT
GGGTATACGACAGTAGAAAAGAAAGTGACACTGAAAGCCGGTGAAAGAATAAGGGTCAATGTAACGATTGCTCCGCAGGT
GACGGAACTGAATGAAGTGGTAGTGTCCACTTCTGCAGTGAGCCGGGTAAATAAATCTGCCTTTAATGCGGTGGCAATTG
ATGCGAAAGGTCTGCATAACAGTACACAAAACCTTTCTGATGCCTTGGCAAAAGTTCCGGGACTCAAACTTCGGGAAGCC
GGAGGAGTGGGGTCGGATATGATTCTTTCGCTGGACGGATTTTCCGGCAAGCACGTCAAGCTGTTTATTGACGGAGTGCC
GCAGGAGGGCGTAGGAAGTTCTTTCGGACTGAACAACATTCCCATTAACTTTGCTGACCGCATCGAAGTGTACCGTGGTG
TAGTGCCTGTCGGGTTCGGGACGGATGCCTTGGGCGGAGTTATCAATATCGTAACCAATAAAAACCGGAAAAACTGGTTT
CTGGACGCATCCTACTCTTACGGCTCGTTCAATACACATAAATCGTACGTCAACTTCGGACAGACCTTCAAGAATGGACT
GACTTACGAAATCAACGCTTTCCAAAACTATTCTGACAACAGTTACTATGTGGATACCCCTGTCGAAGAGTTTTATGAAG
GCGGCGGCTCGGCTATCAATACGGACAAGGTAGAACATGTGAAACGCTTTCATGACAACTATCACAACGAGGCTGTTGTC
GGCAAAGTAGGGCTGGTTGACAAGAAATGGGCGGATCGTCTGATGATCGGACTGACGTACTCGCGTATGTACAAAGAAAT
CCAGACAGGAGTGGTACAAAAGGTCGTATTTGGTGAAAAATATCGTAAGGGAAACTCTCTGATGCCTTCTCTGGAATATC
GTAAACGTAATTTATTCGTCAGGAATCTGGACGTAGCATTCACAGCCAATTACAACCGGAACTTTACGAACAATGTCGAT
ACGGCAACGTACCGTTTCAACTGGCTTGGTGAGAAAACCTCTCTGAAAGGGAGAAAAGGAGAACAGTCATATCAGGATAT
GAAGTCCGACAACGACAACTGGAACGCTACTTTTACCGCCAACTACCACATCGGCACTGCGCATACATTTGTGCTGAACC
ACGTCTTGAACACCTTTCATCGTGAAAATCACAACTCCGTTTCTGTCGATGAATCGAACGCTATCGCCAAAGTGACCCGC
AAAAATATAACCGGATTTTCATACAGGCTGATGCCTTCGGAGCACTGGAATTTATCCGTATTCGGGAAATATTACAACCA
ATATAATGCGGGGCCCGTGTCCGCATCGACCAGTGGAACGAGTAACTACGTCCGTCTCACCAATAATGTAAGCTCTGTGG
GATATGGCGCGGCCGGTACTTATTTCATCCTTTCGGGATTGCAGGCAAAACTTTCTTATGAGAAGGCTTACCGGCTGCCT
ACGAACGAAGAACTTTTTGGTGACGAAGACCTTGAACTGGGAAAAATAGGACTGAATCCGGAAAAAAGTGATAACCTCAA
TTTCAATTTGAGCTATAATCGCCAATTGGGTAAACACGGTCTGTATGTGGAAACCGGACTGATTTACCGGAATACTTCCG
ATTATATCTATCGCAGTATCGAAACAACGAGCAACCGTTCATACGGCTCTTACAGCAACTATGGAAGTGTTGAAACAAAA
GGCTATCATATTTCCGCGCGTTATAATTACTCCTGCTGGGTCAGTATCGGCGGAAACTTTACACAAATGGACGTGCGTGA
CAATGTGGAGAAAACGCAGACCGGGCAGGAAAGTCTGACTTATGGCGCACGTATGCCGAACTTGCCTTACCGTTTTGCCA
ATTCGGACATCTCTTTCTTCTGGCGGAACCTCTGGAAGAAAGGAAATACGCTGACCGTTACCTATGACAATATGTATGTA
CATGGTTTTCCGCTTTACTCCGAAGCATTGGGAGCGGTGGAAACCAAAGACATAGTGCCTACACAGTTTTCACATAATCT
GGGCATCACCTACAGTCTGAAGAATGGACGATATAACGTATCGTTCGAATGTAAAAACTTCACTGACGAAAAACTCTATG
ATAACTTCAGTCTCCAGAAAGCCGGTCGTGCCTTCTATGGGAAAGTGAGAGTGTACTTCGGCGGAAATTGA

Upstream 100 bases:

>100_bases
GGGGATTGTCCCGCATCTCTATTTGTCACTACCTTTGTATCGTTTTAGAATTCGTTATCGGAACTATATAGTAGGTATTA
AGTAAATAGAGTAAAAAGAG

Downstream 100 bases:

>100_bases
AACAGAAAGAATTTTAAACCTGAGAGAAATAAAAACAATTTGATTAACTACAAATACTCAAAAAAAGATGAAGAAGAATT
TTTTGATGTGGAGCTTTGCA

Product: putative TonB-linked outer membrane receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 796; Mature: 796

Protein sequence:

>796_residues
MINKSFLVFLAFLFSVSAFSQHGRSGGMISGRVISTVEKEVVDFATVYLKGTNRGATTDEKGLYHLKAAAGDYTLVVSAI
GYTTVEKKVTLKAGERIRVNVTIAPQVTELNEVVVSTSAVSRVNKSAFNAVAIDAKGLHNSTQNLSDALAKVPGLKLREA
GGVGSDMILSLDGFSGKHVKLFIDGVPQEGVGSSFGLNNIPINFADRIEVYRGVVPVGFGTDALGGVINIVTNKNRKNWF
LDASYSYGSFNTHKSYVNFGQTFKNGLTYEINAFQNYSDNSYYVDTPVEEFYEGGGSAINTDKVEHVKRFHDNYHNEAVV
GKVGLVDKKWADRLMIGLTYSRMYKEIQTGVVQKVVFGEKYRKGNSLMPSLEYRKRNLFVRNLDVAFTANYNRNFTNNVD
TATYRFNWLGEKTSLKGRKGEQSYQDMKSDNDNWNATFTANYHIGTAHTFVLNHVLNTFHRENHNSVSVDESNAIAKVTR
KNITGFSYRLMPSEHWNLSVFGKYYNQYNAGPVSASTSGTSNYVRLTNNVSSVGYGAAGTYFILSGLQAKLSYEKAYRLP
TNEELFGDEDLELGKIGLNPEKSDNLNFNLSYNRQLGKHGLYVETGLIYRNTSDYIYRSIETTSNRSYGSYSNYGSVETK
GYHISARYNYSCWVSIGGNFTQMDVRDNVEKTQTGQESLTYGARMPNLPYRFANSDISFFWRNLWKKGNTLTVTYDNMYV
HGFPLYSEALGAVETKDIVPTQFSHNLGITYSLKNGRYNVSFECKNFTDEKLYDNFSLQKAGRAFYGKVRVYFGGN

Sequences:

>Translated_796_residues
MINKSFLVFLAFLFSVSAFSQHGRSGGMISGRVISTVEKEVVDFATVYLKGTNRGATTDEKGLYHLKAAAGDYTLVVSAI
GYTTVEKKVTLKAGERIRVNVTIAPQVTELNEVVVSTSAVSRVNKSAFNAVAIDAKGLHNSTQNLSDALAKVPGLKLREA
GGVGSDMILSLDGFSGKHVKLFIDGVPQEGVGSSFGLNNIPINFADRIEVYRGVVPVGFGTDALGGVINIVTNKNRKNWF
LDASYSYGSFNTHKSYVNFGQTFKNGLTYEINAFQNYSDNSYYVDTPVEEFYEGGGSAINTDKVEHVKRFHDNYHNEAVV
GKVGLVDKKWADRLMIGLTYSRMYKEIQTGVVQKVVFGEKYRKGNSLMPSLEYRKRNLFVRNLDVAFTANYNRNFTNNVD
TATYRFNWLGEKTSLKGRKGEQSYQDMKSDNDNWNATFTANYHIGTAHTFVLNHVLNTFHRENHNSVSVDESNAIAKVTR
KNITGFSYRLMPSEHWNLSVFGKYYNQYNAGPVSASTSGTSNYVRLTNNVSSVGYGAAGTYFILSGLQAKLSYEKAYRLP
TNEELFGDEDLELGKIGLNPEKSDNLNFNLSYNRQLGKHGLYVETGLIYRNTSDYIYRSIETTSNRSYGSYSNYGSVETK
GYHISARYNYSCWVSIGGNFTQMDVRDNVEKTQTGQESLTYGARMPNLPYRFANSDISFFWRNLWKKGNTLTVTYDNMYV
HGFPLYSEALGAVETKDIVPTQFSHNLGITYSLKNGRYNVSFECKNFTDEKLYDNFSLQKAGRAFYGKVRVYFGGN
>Mature_796_residues
MINKSFLVFLAFLFSVSAFSQHGRSGGMISGRVISTVEKEVVDFATVYLKGTNRGATTDEKGLYHLKAAAGDYTLVVSAI
GYTTVEKKVTLKAGERIRVNVTIAPQVTELNEVVVSTSAVSRVNKSAFNAVAIDAKGLHNSTQNLSDALAKVPGLKLREA
GGVGSDMILSLDGFSGKHVKLFIDGVPQEGVGSSFGLNNIPINFADRIEVYRGVVPVGFGTDALGGVINIVTNKNRKNWF
LDASYSYGSFNTHKSYVNFGQTFKNGLTYEINAFQNYSDNSYYVDTPVEEFYEGGGSAINTDKVEHVKRFHDNYHNEAVV
GKVGLVDKKWADRLMIGLTYSRMYKEIQTGVVQKVVFGEKYRKGNSLMPSLEYRKRNLFVRNLDVAFTANYNRNFTNNVD
TATYRFNWLGEKTSLKGRKGEQSYQDMKSDNDNWNATFTANYHIGTAHTFVLNHVLNTFHRENHNSVSVDESNAIAKVTR
KNITGFSYRLMPSEHWNLSVFGKYYNQYNAGPVSASTSGTSNYVRLTNNVSSVGYGAAGTYFILSGLQAKLSYEKAYRLP
TNEELFGDEDLELGKIGLNPEKSDNLNFNLSYNRQLGKHGLYVETGLIYRNTSDYIYRSIETTSNRSYGSYSNYGSVETK
GYHISARYNYSCWVSIGGNFTQMDVRDNVEKTQTGQESLTYGARMPNLPYRFANSDISFFWRNLWKKGNTLTVTYDNMYV
HGFPLYSEALGAVETKDIVPTQFSHNLGITYSLKNGRYNVSFECKNFTDEKLYDNFSLQKAGRAFYGKVRVYFGGN

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=143, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1788478, Length=134, Percent_Identity=33.5820895522388, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1787723, Length=177, Percent_Identity=29.3785310734463, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 89152; Mature: 89152

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKSFLVFLAFLFSVSAFSQHGRSGGMISGRVISTVEKEVVDFATVYLKGTNRGATTDE
CCCHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHEEEEEEEEECCCCCCCCC
KGLYHLKAAAGDYTLVVSAIGYTTVEKKVTLKAGERIRVNVTIAPQVTELNEVVVSTSAV
CCCEEEEECCCCEEEEEEECCCEEECEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHH
SRVNKSAFNAVAIDAKGLHNSTQNLSDALAKVPGLKLREAGGVGSDMILSLDGFSGKHVK
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCEEE
LFIDGVPQEGVGSSFGLNNIPINFADRIEVYRGVVPVGFGTDALGGVINIVTNKNRKNWF
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEECCCCHHHCCEEEEEECCCCCCEE
LDASYSYGSFNTHKSYVNFGQTFKNGLTYEINAFQNYSDNSYYVDTPVEEFYEGGGSAIN
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCHHHHHCCCCCCCC
TDKVEHVKRFHDNYHNEAVVGKVGLVDKKWADRLMIGLTYSRMYKEIQTGVVQKVVFGEK
CHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
YRKGNSLMPSLEYRKRNLFVRNLDVAFTANYNRNFTNNVDTATYRFNWLGEKTSLKGRKG
HCCCCCCCCCHHHHHCEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
EQSYQDMKSDNDNWNATFTANYHIGTAHTFVLNHVLNTFHRENHNSVSVDESNAIAKVTR
CHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
KNITGFSYRLMPSEHWNLSVFGKYYNQYNAGPVSASTSGTSNYVRLTNNVSSVGYGAAGT
CCCCCEEEEECCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCH
YFILSGLQAKLSYEKAYRLPTNEELFGDEDLELGKIGLNPEKSDNLNFNLSYNRQLGKHG
HHHHHCHHHHCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCC
LYVETGLIYRNTSDYIYRSIETTSNRSYGSYSNYGSVETKGYHISARYNYSCWVSIGGNF
CEEEEEEEEECCCHHEEEEEECCCCCCCCCCCCCCCEEECCEEEEEEECEEEEEEECCCE
TQMDVRDNVEKTQTGQESLTYGARMPNLPYRFANSDISFFWRNLWKKGNTLTVTYDNMYV
EEECCCCCCHHHCCCHHHHHCCCCCCCCCCEECCCCHHHHHHHHHCCCCEEEEEECCEEE
HGFPLYSEALGAVETKDIVPTQFSHNLGITYSLKNGRYNVSFECKNFTDEKLYDNFSLQK
EECCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCHHHHCCCCHHH
AGRAFYGKVRVYFGGN
CCCEEEEEEEEEECCC
>Mature Secondary Structure
MINKSFLVFLAFLFSVSAFSQHGRSGGMISGRVISTVEKEVVDFATVYLKGTNRGATTDE
CCCHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHEEEEEEEEECCCCCCCCC
KGLYHLKAAAGDYTLVVSAIGYTTVEKKVTLKAGERIRVNVTIAPQVTELNEVVVSTSAV
CCCEEEEECCCCEEEEEEECCCEEECEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHH
SRVNKSAFNAVAIDAKGLHNSTQNLSDALAKVPGLKLREAGGVGSDMILSLDGFSGKHVK
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCEEE
LFIDGVPQEGVGSSFGLNNIPINFADRIEVYRGVVPVGFGTDALGGVINIVTNKNRKNWF
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEECCCCHHHCCEEEEEECCCCCCEE
LDASYSYGSFNTHKSYVNFGQTFKNGLTYEINAFQNYSDNSYYVDTPVEEFYEGGGSAIN
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCHHHHHCCCCCCCC
TDKVEHVKRFHDNYHNEAVVGKVGLVDKKWADRLMIGLTYSRMYKEIQTGVVQKVVFGEK
CHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
YRKGNSLMPSLEYRKRNLFVRNLDVAFTANYNRNFTNNVDTATYRFNWLGEKTSLKGRKG
HCCCCCCCCCHHHHHCEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
EQSYQDMKSDNDNWNATFTANYHIGTAHTFVLNHVLNTFHRENHNSVSVDESNAIAKVTR
CHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHH
KNITGFSYRLMPSEHWNLSVFGKYYNQYNAGPVSASTSGTSNYVRLTNNVSSVGYGAAGT
CCCCCEEEEECCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCH
YFILSGLQAKLSYEKAYRLPTNEELFGDEDLELGKIGLNPEKSDNLNFNLSYNRQLGKHG
HHHHHCHHHHCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCC
LYVETGLIYRNTSDYIYRSIETTSNRSYGSYSNYGSVETKGYHISARYNYSCWVSIGGNF
CEEEEEEEEECCCHHEEEEEECCCCCCCCCCCCCCCEEECCEEEEEEECEEEEEEECCCE
TQMDVRDNVEKTQTGQESLTYGARMPNLPYRFANSDISFFWRNLWKKGNTLTVTYDNMYV
EEECCCCCCHHHCCCHHHHHCCCCCCCCCCEECCCCHHHHHHHHHCCCCEEEEEECCEEE
HGFPLYSEALGAVETKDIVPTQFSHNLGITYSLKNGRYNVSFECKNFTDEKLYDNFSLQK
EECCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCHHHHCCCCHHH
AGRAFYGKVRVYFGGN
CCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA