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The map label for this gene is rffG [H]

Identifier: 29347426

GI number: 29347426

Start: 2530168

End: 2531304

Strand: Reverse

Name: rffG [H]

Synonym: BT_2016

Alternate gene names: 29347426

Gene position: 2531304-2530168 (Counterclockwise)

Preceding gene: 29347427

Following gene: 29347423

Centisome position: 40.43

GC content: 48.55

Gene sequence:

>1137_bases
ATGAAAACTTATCTGGTGACCGGTGCCGCAGGATTTATTGGCGCCAATTATATTAAGTATATCTTGGCCAAGCACAGCGA
TATAAAAGTGGTGGTTCTTGACGCCCTGACGTATGCGGGTAATCTGGGAACTATTGCCAAAGATATCGACAACGAACGTT
GTTTCTTTATCAAAGGAGACATTTGCAGCCGCGAAGTGGTGGACAGCCTTTTTGCCGAATACCGTTTCGACTATGTCGTG
AACTTCGCCGCCGAAAGCCATGTAGACCGCAGTATCGAAAACCCGCAACTGTTTCTGATCACCAACATCCTCGGTACGCA
GAATCTGCTGGATTGTGCCCGCCGTGCCTGGGTGATGGGTAAGGACGAACAGGGATATCCTACCTGGAGAAAAGGAGTGC
GCTACCATCAGGTATCTACCGACGAGGTGTACGGCTCACTCGGTGCGGAAGGCTACTTCACGGAAGAAACCCCGCTTTGT
CCGCACAGCCCGTACAGCGCTTCGAAAACCAGTGCCGACATGGTAGTCATGGCTTATCACGACACTTATAAAATGCCGGT
AACGATCACCCGCTGTTCCAACAACTACGGCCCCTATCATTTTCCCGAAAAACTGATTCCGCTGATTATCAAGAATATAC
TCGAAGGCAAACATCTGCCTGTCTACGGTGACGGAAGTAACGTGCGCGACTGGCTTTACGTAGAAGACCACTGCAAAGCG
ATCGACTTGGTAGTGCGCGAAGGCAAAGAGGGCGAGGTGTACAACGTAGGTGGTCACAACGAAGAAACCAATCTTGAAAT
TGTGAAGCTGACCATTGCCACTATCCGTAAACTAATGACCGAGAAACCGGAATATCGTCAGGTCCTTAAAAAGAAAGTGA
AAGGTGAAGACGGAGAAATCTCCGTTGACTGGATCAACAACGATCTGATCACTTTTGTCAAAGACCGTCTGGGGCACGAC
CAGCGTTATGCCATTGATCCCACGAAGATCACGAACGCCTTGGGCTGGTATCCCGAAACGAAATTTGAAGATGGCATCGT
GAAGACAATCGTATGGTATCTTGAAAATCAAGACTGGGTGGAAGAGGTGACCAGCGGAGACTATCAAGGTTATTACGAAA
AGATGTACGGAAAATAA

Upstream 100 bases:

>100_bases
CAATTGCTGCTGCTCGGACAGAAACTCGAAAAGACCGAATACGGTAAATACCTGATAGAACTGTCTAAACAACCCTTGAA
ATAAACATAAATAATCATAT

Downstream 100 bases:

>100_bases
ATCAATGTCGGATCAAGAATCCTATTGATAGCAAAATACCCATTAACAGCATATTACGTGAAGTCTCACCTAGTATGCTG
TTTAATTTTTTACCACTGTG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKTYLVTGAAGFIGANYIKYILAKHSDIKVVVLDALTYAGNLGTIAKDIDNERCFFIKGDICSREVVDSLFAEYRFDYVV
NFAAESHVDRSIENPQLFLITNILGTQNLLDCARRAWVMGKDEQGYPTWRKGVRYHQVSTDEVYGSLGAEGYFTEETPLC
PHSPYSASKTSADMVVMAYHDTYKMPVTITRCSNNYGPYHFPEKLIPLIIKNILEGKHLPVYGDGSNVRDWLYVEDHCKA
IDLVVREGKEGEVYNVGGHNEETNLEIVKLTIATIRKLMTEKPEYRQVLKKKVKGEDGEISVDWINNDLITFVKDRLGHD
QRYAIDPTKITNALGWYPETKFEDGIVKTIVWYLENQDWVEEVTSGDYQGYYEKMYGK

Sequences:

>Translated_378_residues
MKTYLVTGAAGFIGANYIKYILAKHSDIKVVVLDALTYAGNLGTIAKDIDNERCFFIKGDICSREVVDSLFAEYRFDYVV
NFAAESHVDRSIENPQLFLITNILGTQNLLDCARRAWVMGKDEQGYPTWRKGVRYHQVSTDEVYGSLGAEGYFTEETPLC
PHSPYSASKTSADMVVMAYHDTYKMPVTITRCSNNYGPYHFPEKLIPLIIKNILEGKHLPVYGDGSNVRDWLYVEDHCKA
IDLVVREGKEGEVYNVGGHNEETNLEIVKLTIATIRKLMTEKPEYRQVLKKKVKGEDGEISVDWINNDLITFVKDRLGHD
QRYAIDPTKITNALGWYPETKFEDGIVKTIVWYLENQDWVEEVTSGDYQGYYEKMYGK
>Mature_378_residues
MKTYLVTGAAGFIGANYIKYILAKHSDIKVVVLDALTYAGNLGTIAKDIDNERCFFIKGDICSREVVDSLFAEYRFDYVV
NFAAESHVDRSIENPQLFLITNILGTQNLLDCARRAWVMGKDEQGYPTWRKGVRYHQVSTDEVYGSLGAEGYFTEETPLC
PHSPYSASKTSADMVVMAYHDTYKMPVTITRCSNNYGPYHFPEKLIPLIIKNILEGKHLPVYGDGSNVRDWLYVEDHCKA
IDLVVREGKEGEVYNVGGHNEETNLEIVKLTIATIRKLMTEKPEYRQVLKKKVKGEDGEISVDWINNDLITFVKDRLGHD
QRYAIDPTKITNALGWYPETKFEDGIVKTIVWYLENQDWVEEVTSGDYQGYYEKMYGK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=360, Percent_Identity=35.5555555555556, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI42516563, Length=359, Percent_Identity=26.4623955431755, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI48994969, Length=375, Percent_Identity=49.6, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1788353, Length=381, Percent_Identity=46.9816272965879, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1788366, Length=365, Percent_Identity=24.9315068493151, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786974, Length=290, Percent_Identity=23.7931034482759, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=355, Percent_Identity=33.5211267605634, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI115532424, Length=369, Percent_Identity=27.3712737127371, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17539532, Length=361, Percent_Identity=23.2686980609418, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=263, Percent_Identity=25.4752851711027, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356223, Length=358, Percent_Identity=25.1396648044693, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19923002, Length=297, Percent_Identity=28.2828282828283, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24158427, Length=253, Percent_Identity=23.3201581027668, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 43193; Mature: 43193

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLVTGAAGFIGANYIKYILAKHSDIKVVVLDALTYAGNLGTIAKDIDNERCFFIKGD
CCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCHHHHHCCCCEEEEEECC
ICSREVVDSLFAEYRFDYVVNFAAESHVDRSIENPQLFLITNILGTQNLLDCARRAWVMG
CCCHHHHHHHHHHHHHHHEEEEHHHHHHHHCCCCCEEEEEEEHHCCHHHHHHHHHHHCCC
KDEQGYPTWRKGVRYHQVSTDEVYGSLGAEGYFTEETPLCPHSPYSASKTSADMVVMAYH
CCCCCCCHHHHCCEEEECCHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEC
DTYKMPVTITRCSNNYGPYHFPEKLIPLIIKNILEGKHLPVYGDGSNVRDWLYVEDHCKA
CCEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEHHHHHE
IDLVVREGKEGEVYNVGGHNEETNLEIVKLTIATIRKLMTEKPEYRQVLKKKVKGEDGEI
EEHHHHCCCCCCEEECCCCCCCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCCE
SVDWINNDLITFVKDRLGHDQRYAIDPTKITNALGWYPETKFEDGIVKTIVWYLENQDWV
EEEEECCCHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHH
EEVTSGDYQGYYEKMYGK
HHHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MKTYLVTGAAGFIGANYIKYILAKHSDIKVVVLDALTYAGNLGTIAKDIDNERCFFIKGD
CCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCHHHHHCCCCEEEEEECC
ICSREVVDSLFAEYRFDYVVNFAAESHVDRSIENPQLFLITNILGTQNLLDCARRAWVMG
CCCHHHHHHHHHHHHHHHEEEEHHHHHHHHCCCCCEEEEEEEHHCCHHHHHHHHHHHCCC
KDEQGYPTWRKGVRYHQVSTDEVYGSLGAEGYFTEETPLCPHSPYSASKTSADMVVMAYH
CCCCCCCHHHHCCEEEECCHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEC
DTYKMPVTITRCSNNYGPYHFPEKLIPLIIKNILEGKHLPVYGDGSNVRDWLYVEDHCKA
CCEECCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEHHHHHE
IDLVVREGKEGEVYNVGGHNEETNLEIVKLTIATIRKLMTEKPEYRQVLKKKVKGEDGEI
EEHHHHCCCCCCEEECCCCCCCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCCE
SVDWINNDLITFVKDRLGHDQRYAIDPTKITNALGWYPETKFEDGIVKTIVWYLENQDWV
EEEEECCCHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHH
EEVTSGDYQGYYEKMYGK
HHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]