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The map label for this gene is mdtE [H]

Identifier: 29347377

GI number: 29347377

Start: 2469908

End: 2471050

Strand: Reverse

Name: mdtE [H]

Synonym: BT_1967

Alternate gene names: 29347377

Gene position: 2471050-2469908 (Counterclockwise)

Preceding gene: 29347382

Following gene: 29347376

Centisome position: 39.47

GC content: 51.53

Gene sequence:

>1143_bases
ATGAGTAAAAAGGTATGTAAAATGAAGCAAGGGCTATTATTGCTCGGCTGTCTGGTTGCTGCTGCAGGTTGTAAGCAAGC
TCCACCCACACAAATGGAAACAGGATATGAAGTTATGACCGTCTCTCCGACCGACCGGATGATTTCGAGTGCCTATTCGG
CGACTATCCGCGGACGTCAGGACATCGATATTTATCCACAGGTAGGCGGTACGCTGACAAAAGTCAGTGTCACCGAAGGA
CAGCGGGTGAAGAGCGGACAGACGCTGTTCATCATCGACCAAGTGCCGTATGAGGCAGCCCTGCAGACGGCAGTGGCGAA
TGTAGAGTCAGCCAAAGCGTCTCTGGCGACGGCACAGCTGACTTACGACAGCAAAGAGGAACTGTATAAGGAGAATGTAG
TTTCCGCTTTCGATCTGAGTACGGCAAAAAATTCTCTTCTGGCTGCCAAAGCGCAACTGGCGCAGGCCAAGGCACAAGAA
GTGAGCGCCCGTAATAACCTTTCTTATACGGTAGTGAAGAGTCCGGCAGACGGAGTGGTAGGTACATTGCCTTATCGCGT
AGGTGCGTTGGTAAGTTCCAGTATCCCCGAACCGCTGACCACCGTTTCCGACAATTCGGATATGTACGTTTACTTCTCGA
TGACGGAAAACCAGTTGCTGGGATTGATTCGCCAGTATGGCTCTAAAGACGAGGCGCTGAAAAGTATGCCCGCCATCGAT
TTGCAGCTGAACGATAAATCGGCTTATCCCGAACAGGGACAGATCGAGTCTATCAGCGGAGTCATTGACCGTTCGACGGG
TACTGTCAGCCTGCGTGCCGTTTTCCCTAATAAGGACGGATTGCTGCACAGCGGCGGTGCGGGTAACGTAGTGATTCCGG
TACAGAAAACGGGAGCTTTGGTGATCCCGCAGGGAGCTACTTTCGAAATACAGGATAAGAGATATGTATATAAGGTAGTA
GACGGCAAAGCGCAGTCCAGTCAGGTGCAGGTGACCCGTGTGAACGGCGGCCGCGAATTTATCGTGGATGAAGGACTGTC
TCCGGGCGATGTGATTGTGGCGGAAGGCGTAGGATTGCTTCGTGAAGGTACTCCGATTAAGGCAAAGACTGCCCAGGCAT
CTACGACAACTACTGAAAATTAA

Upstream 100 bases:

>100_bases
ATTGTTCCGAAAATGAACAAAACTCTAAACACTATGACCAGTTTTCTATGGTGAAAAGAGTCTACCTTTGCCGCGATCAA
AAATTTTAATAGGTAACACA

Downstream 100 bases:

>100_bases
ACAAAGGAGGAATCATCCATGAATTTAAGAACCTTTATTGAACGCCCCGTATTATCGGCAGTCATCTCTATTACGATTGT
CGTTGTGGGTATCATCGGGC

Product: multidrug efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSKKVCKMKQGLLLLGCLVAAAGCKQAPPTQMETGYEVMTVSPTDRMISSAYSATIRGRQDIDIYPQVGGTLTKVSVTEG
QRVKSGQTLFIIDQVPYEAALQTAVANVESAKASLATAQLTYDSKEELYKENVVSAFDLSTAKNSLLAAKAQLAQAKAQE
VSARNNLSYTVVKSPADGVVGTLPYRVGALVSSSIPEPLTTVSDNSDMYVYFSMTENQLLGLIRQYGSKDEALKSMPAID
LQLNDKSAYPEQGQIESISGVIDRSTGTVSLRAVFPNKDGLLHSGGAGNVVIPVQKTGALVIPQGATFEIQDKRYVYKVV
DGKAQSSQVQVTRVNGGREFIVDEGLSPGDVIVAEGVGLLREGTPIKAKTAQASTTTTEN

Sequences:

>Translated_380_residues
MSKKVCKMKQGLLLLGCLVAAAGCKQAPPTQMETGYEVMTVSPTDRMISSAYSATIRGRQDIDIYPQVGGTLTKVSVTEG
QRVKSGQTLFIIDQVPYEAALQTAVANVESAKASLATAQLTYDSKEELYKENVVSAFDLSTAKNSLLAAKAQLAQAKAQE
VSARNNLSYTVVKSPADGVVGTLPYRVGALVSSSIPEPLTTVSDNSDMYVYFSMTENQLLGLIRQYGSKDEALKSMPAID
LQLNDKSAYPEQGQIESISGVIDRSTGTVSLRAVFPNKDGLLHSGGAGNVVIPVQKTGALVIPQGATFEIQDKRYVYKVV
DGKAQSSQVQVTRVNGGREFIVDEGLSPGDVIVAEGVGLLREGTPIKAKTAQASTTTTEN
>Mature_379_residues
SKKVCKMKQGLLLLGCLVAAAGCKQAPPTQMETGYEVMTVSPTDRMISSAYSATIRGRQDIDIYPQVGGTLTKVSVTEGQ
RVKSGQTLFIIDQVPYEAALQTAVANVESAKASLATAQLTYDSKEELYKENVVSAFDLSTAKNSLLAAKAQLAQAKAQEV
SARNNLSYTVVKSPADGVVGTLPYRVGALVSSSIPEPLTTVSDNSDMYVYFSMTENQLLGLIRQYGSKDEALKSMPAIDL
QLNDKSAYPEQGQIESISGVIDRSTGTVSLRAVFPNKDGLLHSGGAGNVVIPVQKTGALVIPQGATFEIQDKRYVYKVVD
GKAQSSQVQVTRVNGGREFIVDEGLSPGDVIVAEGVGLLREGTPIKAKTAQASTTTTEN

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=373, Percent_Identity=28.686327077748, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1786668, Length=377, Percent_Identity=28.3819628647215, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082036, Length=336, Percent_Identity=26.1904761904762, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789665, Length=313, Percent_Identity=29.7124600638978, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081796, Length=338, Percent_Identity=28.698224852071, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789637, Length=189, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081951, Length=163, Percent_Identity=30.6748466257669, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40384; Mature: 40253

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVCKMKQGLLLLGCLVAAAGCKQAPPTQMETGYEVMTVSPTDRMISSAYSATIRGRQ
CCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHEECCCC
DIDIYPQVGGTLTKVSVTEGQRVKSGQTLFIIDQVPYEAALQTAVANVESAKASLATAQL
CCEEECCCCCEEEEEEECCCCEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEE
TYDSKEELYKENVVSAFDLSTAKNSLLAAKAQLAQAKAQEVSARNNLSYTVVKSPADGVV
ECCCHHHHHHHHCHHHEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
GTLPYRVGALVSSSIPEPLTTVSDNSDMYVYFSMTENQLLGLIRQYGSKDEALKSMPAID
ECCCHHHHHHHHCCCCCCCEEECCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCEE
LQLNDKSAYPEQGQIESISGVIDRSTGTVSLRAVFPNKDGLLHSGGAGNVVIPVQKTGAL
EEECCCCCCCCCCCCCHHHHEEECCCCEEEEEEECCCCCCEEECCCCCCEEEEEECCCEE
VIPQGATFEIQDKRYVYKVVDGKAQSSQVQVTRVNGGREFIVDEGLSPGDVIVAEGVGLL
EECCCCEEEECCCEEEEEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCEEEECCCCCC
REGTPIKAKTAQASTTTTEN
CCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
SKKVCKMKQGLLLLGCLVAAAGCKQAPPTQMETGYEVMTVSPTDRMISSAYSATIRGRQ
CCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHEECCCC
DIDIYPQVGGTLTKVSVTEGQRVKSGQTLFIIDQVPYEAALQTAVANVESAKASLATAQL
CCEEECCCCCEEEEEEECCCCEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEE
TYDSKEELYKENVVSAFDLSTAKNSLLAAKAQLAQAKAQEVSARNNLSYTVVKSPADGVV
ECCCHHHHHHHHCHHHEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
GTLPYRVGALVSSSIPEPLTTVSDNSDMYVYFSMTENQLLGLIRQYGSKDEALKSMPAID
ECCCHHHHHHHHCCCCCCCEEECCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCEE
LQLNDKSAYPEQGQIESISGVIDRSTGTVSLRAVFPNKDGLLHSGGAGNVVIPVQKTGAL
EEECCCCCCCCCCCCCHHHHEEECCCCEEEEEEECCCCCCEEECCCCCCEEEEEECCCEE
VIPQGATFEIQDKRYVYKVVDGKAQSSQVQVTRVNGGREFIVDEGLSPGDVIVAEGVGLL
EECCCCEEEECCCEEEEEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCEEEECCCCCC
REGTPIKAKTAQASTTTTEN
CCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]