Click here to switch to the map view.

The map label for this gene is cysK [H]

Identifier: 29347262

GI number: 29347262

Start: 2324172

End: 2325125

Strand: Reverse

Name: cysK [H]

Synonym: BT_1852

Alternate gene names: 29347262

Gene position: 2325125-2324172 (Counterclockwise)

Preceding gene: 29347266

Following gene: 29347261

Centisome position: 37.14

GC content: 50.63

Gene sequence:

>954_bases
ATGATGGCAAAAATTGCGAATAAATTAACAGATTTAGTGGGTAATACACCTCTAATGGAACTTTCCGGATATAGTGGAAA
GTATGGTCTTAATCAGAATATTATCGCTAAACTGGAGGCTTTTAATCCGGCGGGAAGCGTAAAAGACAGAGTCGCTCTTT
CTATGATTGAAGATGCGGAGGCCCGCGGGGCACTGAAACCCGGTGCCACCATTATCGAACCGACCAGCGGAAATACGGGA
GTCGGACTGGCGATGGTAGCTACCATTAAAGGGTATCACTTGATATTGACTATGCCCGAAACGATGAGCTTGGAACGGCG
AAACCTGTTGAAAGCACTGGGGGCGCAGATTGTGCTAACAGATGGCTTGGGCGGGATGGCTGCTTCTATTGCCAAGGCGC
AGGAGTTGCGCGATAGTATCCCCGGATCTGTGATCCTGCAACAGTTTGAAAATCCTTCGAATGCTGCTGTCCATGAGCGG
ACAACAGGAGAGGAAATCTGGCGGGATACGGATGGTGAAGTGGCAGTGTTCGTTGCCGGAGTAGGAACCGGAGGTACGAT
TTGCGGCGTGGCACGCGCCTTAAAAAAACATAATCCGGATGTTCATATCGTTGCGGTTGAACCTGCGTCGTCTCCCATAT
TGGCAGGAGGTCAGGCAGCATCACACCGCATACAGGGAATCGGGGCGAATTTTATTCCGAAACTTTATGATGCTTCTGTA
GTGGATGAAGTGATAGGTGTGCCCGATGACGAAGCCATTCGTGCGGGGCGAGAGCTGGCTGCGACAGAAGGTTTGCTGGC
CGGAATCTCTTCGGGAGCGGCCGTATATGCAGCCCGACAGTTGGCACAGCGTCCGGAGTTCAGGAACAAAAAAATCGTTG
CGTTGTTACCTGATACGGGAGAACGCTATCTGTCGACCGAGTTGTTCGCTTTTGATGCCTATCCTTTGGATTAA

Upstream 100 bases:

>100_bases
GATTCTCTTTTTCATATCTATAAACTTAAAAATATTGAACGGAGACAAAGGTACAATTCTTTTCTGTATCTTTGCGCATC
AACCTGATAAAACGAGGTGA

Downstream 100 bases:

>100_bases
TATTTGAATCATGATAAGAATTATTTTAATTTTATTTCTTAGCTTTCCGGTTGCCCTGTGTGCGCAGACCTATCAGCAAT
TATCAGAAAAGGCAATTGAA

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MMAKIANKLTDLVGNTPLMELSGYSGKYGLNQNIIAKLEAFNPAGSVKDRVALSMIEDAEARGALKPGATIIEPTSGNTG
VGLAMVATIKGYHLILTMPETMSLERRNLLKALGAQIVLTDGLGGMAASIAKAQELRDSIPGSVILQQFENPSNAAVHER
TTGEEIWRDTDGEVAVFVAGVGTGGTICGVARALKKHNPDVHIVAVEPASSPILAGGQAASHRIQGIGANFIPKLYDASV
VDEVIGVPDDEAIRAGRELAATEGLLAGISSGAAVYAARQLAQRPEFRNKKIVALLPDTGERYLSTELFAFDAYPLD

Sequences:

>Translated_317_residues
MMAKIANKLTDLVGNTPLMELSGYSGKYGLNQNIIAKLEAFNPAGSVKDRVALSMIEDAEARGALKPGATIIEPTSGNTG
VGLAMVATIKGYHLILTMPETMSLERRNLLKALGAQIVLTDGLGGMAASIAKAQELRDSIPGSVILQQFENPSNAAVHER
TTGEEIWRDTDGEVAVFVAGVGTGGTICGVARALKKHNPDVHIVAVEPASSPILAGGQAASHRIQGIGANFIPKLYDASV
VDEVIGVPDDEAIRAGRELAATEGLLAGISSGAAVYAARQLAQRPEFRNKKIVALLPDTGERYLSTELFAFDAYPLD
>Mature_317_residues
MMAKIANKLTDLVGNTPLMELSGYSGKYGLNQNIIAKLEAFNPAGSVKDRVALSMIEDAEARGALKPGATIIEPTSGNTG
VGLAMVATIKGYHLILTMPETMSLERRNLLKALGAQIVLTDGLGGMAASIAKAQELRDSIPGSVILQQFENPSNAAVHER
TTGEEIWRDTDGEVAVFVAGVGTGGTICGVARALKKHNPDVHIVAVEPASSPILAGGQAASHRIQGIGANFIPKLYDASV
VDEVIGVPDDEAIRAGRELAATEGLLAGISSGAAVYAARQLAQRPEFRNKKIVALLPDTGERYLSTELFAFDAYPLD

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=321, Percent_Identity=42.3676012461059, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI295821200, Length=321, Percent_Identity=42.3676012461059, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI4557415, Length=321, Percent_Identity=42.3676012461059, Blast_Score=245, Evalue=4e-65,
Organism=Escherichia coli, GI1788754, Length=318, Percent_Identity=54.7169811320755, Blast_Score=296, Evalue=9e-82,
Organism=Escherichia coli, GI2367138, Length=307, Percent_Identity=41.6938110749186, Blast_Score=222, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17535051, Length=301, Percent_Identity=48.8372093023256, Blast_Score=287, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI115535073, Length=299, Percent_Identity=52.8428093645485, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17562970, Length=301, Percent_Identity=46.5116279069767, Blast_Score=273, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=46.7796610169492, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17561720, Length=301, Percent_Identity=45.1827242524917, Blast_Score=233, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=46.0093896713615, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=319, Percent_Identity=41.0658307210031, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI25147552, Length=315, Percent_Identity=36.5079365079365, Blast_Score=173, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=42.0168067226891, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321594, Length=312, Percent_Identity=41.025641025641, Blast_Score=223, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321449, Length=341, Percent_Identity=36.6568914956012, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24643623, Length=322, Percent_Identity=38.5093167701863, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI20129101, Length=322, Percent_Identity=38.5093167701863, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33270; Mature: 33270

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKIANKLTDLVGNTPLMELSGYSGKYGLNQNIIAKLEAFNPAGSVKDRVALSMIEDAE
CHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
ARGALKPGATIIEPTSGNTGVGLAMVATIKGYHLILTMPETMSLERRNLLKALGAQIVLT
HCCCCCCCCEEEECCCCCCCCHHHEEEECCCEEEEEECCCHHHHHHHHHHHHCCCEEEEE
DGLGGMAASIAKAQELRDSIPGSVILQQFENPSNAAVHERTTGEEIWRDTDGEVAVFVAG
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCHHHHHCCCCCCEEEEEEE
VGTGGTICGVARALKKHNPDVHIVAVEPASSPILAGGQAASHRIQGIGANFIPKLYDASV
CCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHHHHHHCCCCHHHHHHHHHHH
VDEVIGVPDDEAIRAGRELAATEGLLAGISSGAAVYAARQLAQRPEFRNKKIVALLPDTG
HHHHHCCCCHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
ERYLSTELFAFDAYPLD
CHHHHHEEEEEECCCCC
>Mature Secondary Structure
MMAKIANKLTDLVGNTPLMELSGYSGKYGLNQNIIAKLEAFNPAGSVKDRVALSMIEDAE
CHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
ARGALKPGATIIEPTSGNTGVGLAMVATIKGYHLILTMPETMSLERRNLLKALGAQIVLT
HCCCCCCCCEEEECCCCCCCCHHHEEEECCCEEEEEECCCHHHHHHHHHHHHCCCEEEEE
DGLGGMAASIAKAQELRDSIPGSVILQQFENPSNAAVHERTTGEEIWRDTDGEVAVFVAG
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCHHHHHCCCCCCEEEEEEE
VGTGGTICGVARALKKHNPDVHIVAVEPASSPILAGGQAASHRIQGIGANFIPKLYDASV
CCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHHHHHHCCCCHHHHHHHHHHH
VDEVIGVPDDEAIRAGRELAATEGLLAGISSGAAVYAARQLAQRPEFRNKKIVALLPDTG
HHHHHCCCCHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
ERYLSTELFAFDAYPLD
CHHHHHEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]