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The map label for this gene is sigW [H]

Identifier: 29347227

GI number: 29347227

Start: 2279420

End: 2279968

Strand: Reverse

Name: sigW [H]

Synonym: BT_1817

Alternate gene names: 29347227

Gene position: 2279968-2279420 (Counterclockwise)

Preceding gene: 29347229

Following gene: 29347226

Centisome position: 36.42

GC content: 42.08

Gene sequence:

>549_bases
ATGGAACAGAAAGACGAATCATACTATATCGAACGCATACTGGATGGAGAGACGGAATACTTTTCCGTATTTCTTGACCG
CTACAGTCGTCCGTTGTATACGTTGGTAGTGCAGATCGTCGGTTGTCCCGAAGATGCGGAGGAACTACTTCAGGATATTT
TTCTGAAAGCTTTCCGCAACTTGAATCGTTATAAAGGCGAGTGCCGTTTTTCTACATGGATTTATCGCATCGCTTACAAT
GCCGCTATCTCGGCTACCCGGAAAAAGAAACAGGAGTTTCTTTATATAGAAGAGAATACGATTAATAATGTACCCGATGA
GATGGCGGACAATGTGCTTGCTCCGGCAGAAACCGAAGAACAACTGGAGCGTTTGGAGATGGCGATAGATCAGTTGAGCG
GAGAAGAGAAAGCATTGATTACTTTGTTTTATTATGAAGAAAAGTCAATGGAAGAGATCGGTGAGGTGCTGAAACTTTCG
ATCAGTAATGTGAAAGTACGGCTTCATCGTACCCGGAAAAAGATTTGTGTATTGATGAATAATAAATAG

Upstream 100 bases:

>100_bases
ACGGAAAAAGGTTACACTGAAAAGCGGATAATTTATAATCCTGTATTTTTTCTTTCCCGGATTGTAACCTTTTGTATCGG
GCAGCGTCTAAAGAGTCAAG

Downstream 100 bases:

>100_bases
AAGAGAGGATATGGAAGAGGAAAGAAAGATAAACGAGAATACGGGCAATATGCTGAAACAAGCATTGGAACGGAGGCAAA
CGGAACGACTGTCTTCCAAT

Product: RNA polymerase ECF-type sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MEQKDESYYIERILDGETEYFSVFLDRYSRPLYTLVVQIVGCPEDAEELLQDIFLKAFRNLNRYKGECRFSTWIYRIAYN
AAISATRKKKQEFLYIEENTINNVPDEMADNVLAPAETEEQLERLEMAIDQLSGEEKALITLFYYEEKSMEEIGEVLKLS
ISNVKVRLHRTRKKICVLMNNK

Sequences:

>Translated_182_residues
MEQKDESYYIERILDGETEYFSVFLDRYSRPLYTLVVQIVGCPEDAEELLQDIFLKAFRNLNRYKGECRFSTWIYRIAYN
AAISATRKKKQEFLYIEENTINNVPDEMADNVLAPAETEEQLERLEMAIDQLSGEEKALITLFYYEEKSMEEIGEVLKLS
ISNVKVRLHRTRKKICVLMNNK
>Mature_182_residues
MEQKDESYYIERILDGETEYFSVFLDRYSRPLYTLVVQIVGCPEDAEELLQDIFLKAFRNLNRYKGECRFSTWIYRIAYN
AAISATRKKKQEFLYIEENTINNVPDEMADNVLAPAETEEQLERLEMAIDQLSGEEKALITLFYYEEKSMEEIGEVLKLS
ISNVKVRLHRTRKKICVLMNNK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21505; Mature: 21505

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKDESYYIERILDGETEYFSVFLDRYSRPLYTLVVQIVGCPEDAEELLQDIFLKAFRN
CCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LNRYKGECRFSTWIYRIAYNAAISATRKKKQEFLYIEENTINNVPDEMADNVLAPAETEE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHCCCCCCCCHHH
QLERLEMAIDQLSGEEKALITLFYYEEKSMEEIGEVLKLSISNVKVRLHRTRKKICVLMN
HHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
NK
CC
>Mature Secondary Structure
MEQKDESYYIERILDGETEYFSVFLDRYSRPLYTLVVQIVGCPEDAEELLQDIFLKAFRN
CCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LNRYKGECRFSTWIYRIAYNAAISATRKKKQEFLYIEENTINNVPDEMADNVLAPAETEE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHCCCCCCCCHHH
QLERLEMAIDQLSGEEKALITLFYYEEKSMEEIGEVLKLSISNVKVRLHRTRKKICVLMN
HHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]