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The map label for this gene is opuAB [H]

Identifier: 29347160

GI number: 29347160

Start: 2160624

End: 2161454

Strand: Reverse

Name: opuAB [H]

Synonym: BT_1750

Alternate gene names: 29347160

Gene position: 2161454-2160624 (Counterclockwise)

Preceding gene: 29347161

Following gene: 29347159

Centisome position: 34.53

GC content: 46.57

Gene sequence:

>831_bases
ATGATAAATATCGGACAATATATAGAGACTGCCATTAATTGGCTCACAGAACATTTTGCTTCCTTTTTTGATGCTCTCAG
CATGGGGATCGGAGGATTCATAGACGGTTTTCAGCACGTACTGTTTGGAATACCTTTTTATATCACAATTGCAGTGCTGG
CTGCGCTCGCCTGGTTTAAGTCGGGTAAGGGGACAGCCGTATTTACCCTCTTGGGTTTATTGCTTATTTACGGCATGGGC
TTTTGGGAAGAAACGATGCAGACATTGGCATTGGTACTTTCTTCCACTTGTCTGGCATTGCTTTTAGGCGTTCCGTTGGG
AATCTGGACTGCCAACAGTGATCGTTGTAACAAGATAATGCGCCCTGTCCTCGACTTTATGCAGACAATGCCTGCCTTTG
TCTATCTGATTCCTGCCGTTCTATTCTTCGGATTGGGAACTGTGCCGGGAGCTTTTGCCACCATCATCTTTGCAATGCCT
CCTGTGGTTCGTCTGACCGGTCTGGGCATTCGTCAGGTTCCTAAAAACGTAGTAGAAGCCTCCCGCTCTTTTGGAGCTAC
TCCCTGGCAGCTTCTTTATAAGGTGCAATTGCCTTTGGCGTTGCCTACTATTCTGACGGGAATCAACCAGACGATCATGA
TGTCACTTTCGATGGTAGTGATTGCTGCCATGATTTCTGCCGGAGGATTGGGAGAAATCGTGCTGAAAGGTATCACCCAA
ATGAAAATCGGACTCGGATTTGAAGGCGGTATCGCAGTCGTGATATTGGCGATCGTGCTGGACCGCATCACTCAGGGTAT
GGCAGATAACAGAAAAAGTAAAAAGAAATAA

Upstream 100 bases:

>100_bases
GGGAGTTTGAAGGCGTAGTGCCACTTTCCTCATTGATCATCGAAGTAACGGGAAAAGATAAAGAAGAAATTAATGAAATC
ATTCAAAACGCAATAGAATT

Downstream 100 bases:

>100_bases
GAATCACACCAAAGAAAGGAATAATCAATGAATAAGATAAAAATGATTGCGGCAATGTTTATTGCCGGCATACTCTTAAC
CTTCGTTTCCTGCGGAGACA

Product: glycine betaine/L-proline transport system permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MINIGQYIETAINWLTEHFASFFDALSMGIGGFIDGFQHVLFGIPFYITIAVLAALAWFKSGKGTAVFTLLGLLLIYGMG
FWEETMQTLALVLSSTCLALLLGVPLGIWTANSDRCNKIMRPVLDFMQTMPAFVYLIPAVLFFGLGTVPGAFATIIFAMP
PVVRLTGLGIRQVPKNVVEASRSFGATPWQLLYKVQLPLALPTILTGINQTIMMSLSMVVIAAMISAGGLGEIVLKGITQ
MKIGLGFEGGIAVVILAIVLDRITQGMADNRKSKKK

Sequences:

>Translated_276_residues
MINIGQYIETAINWLTEHFASFFDALSMGIGGFIDGFQHVLFGIPFYITIAVLAALAWFKSGKGTAVFTLLGLLLIYGMG
FWEETMQTLALVLSSTCLALLLGVPLGIWTANSDRCNKIMRPVLDFMQTMPAFVYLIPAVLFFGLGTVPGAFATIIFAMP
PVVRLTGLGIRQVPKNVVEASRSFGATPWQLLYKVQLPLALPTILTGINQTIMMSLSMVVIAAMISAGGLGEIVLKGITQ
MKIGLGFEGGIAVVILAIVLDRITQGMADNRKSKKK
>Mature_276_residues
MINIGQYIETAINWLTEHFASFFDALSMGIGGFIDGFQHVLFGIPFYITIAVLAALAWFKSGKGTAVFTLLGLLLIYGMG
FWEETMQTLALVLSSTCLALLLGVPLGIWTANSDRCNKIMRPVLDFMQTMPAFVYLIPAVLFFGLGTVPGAFATIIFAMP
PVVRLTGLGIRQVPKNVVEASRSFGATPWQLLYKVQLPLALPTILTGINQTIMMSLSMVVIAAMISAGGLGEIVLKGITQ
MKIGLGFEGGIAVVILAIVLDRITQGMADNRKSKKK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=274, Percent_Identity=51.0948905109489, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1788451, Length=212, Percent_Identity=32.0754716981132, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788449, Length=205, Percent_Identity=26.8292682926829, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=30.3225806451613, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29796; Mature: 29796

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]