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The map label for this gene is wcaL [C]

Identifier: 29347122

GI number: 29347122

Start: 2108693

End: 2109871

Strand: Reverse

Name: wcaL [C]

Synonym: BT_1712

Alternate gene names: 29347122

Gene position: 2109871-2108693 (Counterclockwise)

Preceding gene: 29347123

Following gene: 29347121

Centisome position: 33.7

GC content: 32.23

Gene sequence:

>1179_bases
ATGAAAAAGGTAGATATAATTCATGGTGGATGGCTTAAGGCTCCTAATGGGGCTAGTTCTGTTCTTAGGACTTTAGCTGA
ATCTACTTCAAAATTTGAAGAATACGGTATTCAGCTTTCGGTTTATTCAATGGATTTGATTGTGTTAAAGTCATTTGAAA
ATATAGCAGCTATAAATCAACGGAAAGGATTACGGTACTTTATTAAAAAGAATGCTAATGCAAATTCTATACTAGCATTC
ATTTCTATTTATGCAATATATTTGCGGCATGCTCGTAAGATTGTTAAATATTATTTTGATCAAGGGCTTGAGGAATCGAG
AATCTTGTTTATACATGATATTTTTACATGTTATTACTATTTGAAGTATCGATCTAGAAGACAAAAAACCGTGTTGGTAT
TACACAATAATGGTGATACTTTTAATATGCTTAGACAATATTATCCTGTGCTTAATAAATCAAGGCTTTATAGTATATTA
TTATACATTGAAAGGAAGGTTTTATCTGAAGTGGATAAGGTGTTGTTTGTTGCTGAGAATCCTAAAGATACTTTTGTACA
ATTACATCCAGATATTCCTATTGAAAAAGTAGGATTTGTTTATAATGGCATTTTGAGCAAAGAAATGCATATATGTCCGG
AAAAACATCTAGGGCCACTTGAAATCTGCTGTGTGGGATCTGTTTCAAAACGTAAAGGGCAAGATATGATTGTTGAAGCT
CTTGTGAAGATGTCTCCTGTTCAAAGAGAGAAAGTGCATTTTACCATTGTAGGTGATGGTACATTAAGGGGGGAATTAGA
AAAGTTGTGTTTTGAGAAAGGAATCTCTAAATATATAGATTTTATAGGTGTTTCGAATCAAGTAGAAAATTATTTGTTGA
GGAGTGATATTTTTATGTTACCTAGTAGAGATGAAGGATTCCCTATTTCTATTTTGGAGGCTATGCGAGCAGGACTTCCT
ATTATATCTACTAATATTGCAGGTATTCCGGAAATGGTTTTTTCCGGGATCAATGGAATTGTAATTTCGCCATGTCTTGA
AGATATATATGATATCTTGTGTCATATTGAATCTTATAATTGGAGTGCAATGGGTAAACTCTCTTATGAACTATATCAAC
AGAAATTTACGCTTGATTCAATGATTGAATCTTATGCTAATATTTTGAACGCTATATGA

Upstream 100 bases:

>100_bases
AAAAAGAGGTGGTGAAGGTGTTTTTAGAGAATTTGTTGAAAAAGTTCTTGGTATAAATTTGGAAGATTTTATTGCTGTGA
TTCAATAGTTGTTGTAAAAG

Downstream 100 bases:

>100_bases
AATATGTCTTTTGGATACATAGTAGTACTTCGTATTATATGGCAAAAGCTATTATAGCAAAAGAAAATTTAGATTGCCAT
AATGTTGTTTATTTGATTGA

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKKVDIIHGGWLKAPNGASSVLRTLAESTSKFEEYGIQLSVYSMDLIVLKSFENIAAINQRKGLRYFIKKNANANSILAF
ISIYAIYLRHARKIVKYYFDQGLEESRILFIHDIFTCYYYLKYRSRRQKTVLVLHNNGDTFNMLRQYYPVLNKSRLYSIL
LYIERKVLSEVDKVLFVAENPKDTFVQLHPDIPIEKVGFVYNGILSKEMHICPEKHLGPLEICCVGSVSKRKGQDMIVEA
LVKMSPVQREKVHFTIVGDGTLRGELEKLCFEKGISKYIDFIGVSNQVENYLLRSDIFMLPSRDEGFPISILEAMRAGLP
IISTNIAGIPEMVFSGINGIVISPCLEDIYDILCHIESYNWSAMGKLSYELYQQKFTLDSMIESYANILNAI

Sequences:

>Translated_392_residues
MKKVDIIHGGWLKAPNGASSVLRTLAESTSKFEEYGIQLSVYSMDLIVLKSFENIAAINQRKGLRYFIKKNANANSILAF
ISIYAIYLRHARKIVKYYFDQGLEESRILFIHDIFTCYYYLKYRSRRQKTVLVLHNNGDTFNMLRQYYPVLNKSRLYSIL
LYIERKVLSEVDKVLFVAENPKDTFVQLHPDIPIEKVGFVYNGILSKEMHICPEKHLGPLEICCVGSVSKRKGQDMIVEA
LVKMSPVQREKVHFTIVGDGTLRGELEKLCFEKGISKYIDFIGVSNQVENYLLRSDIFMLPSRDEGFPISILEAMRAGLP
IISTNIAGIPEMVFSGINGIVISPCLEDIYDILCHIESYNWSAMGKLSYELYQQKFTLDSMIESYANILNAI
>Mature_392_residues
MKKVDIIHGGWLKAPNGASSVLRTLAESTSKFEEYGIQLSVYSMDLIVLKSFENIAAINQRKGLRYFIKKNANANSILAF
ISIYAIYLRHARKIVKYYFDQGLEESRILFIHDIFTCYYYLKYRSRRQKTVLVLHNNGDTFNMLRQYYPVLNKSRLYSIL
LYIERKVLSEVDKVLFVAENPKDTFVQLHPDIPIEKVGFVYNGILSKEMHICPEKHLGPLEICCVGSVSKRKGQDMIVEA
LVKMSPVQREKVHFTIVGDGTLRGELEKLCFEKGISKYIDFIGVSNQVENYLLRSDIFMLPSRDEGFPISILEAMRAGLP
IISTNIAGIPEMVFSGINGIVISPCLEDIYDILCHIESYNWSAMGKLSYELYQQKFTLDSMIESYANILNAI

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=159, Percent_Identity=28.9308176100629, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17532709, Length=191, Percent_Identity=29.8429319371728, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 44903; Mature: 44903

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVDIIHGGWLKAPNGASSVLRTLAESTSKFEEYGIQLSVYSMDLIVLKSFENIAAINQ
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHH
RKGLRYFIKKNANANSILAFISIYAIYLRHARKIVKYYFDQGLEESRILFIHDIFTCYYY
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
LKYRSRRQKTVLVLHNNGDTFNMLRQYYPVLNKSRLYSILLYIERKVLSEVDKVLFVAEN
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEEECC
PKDTFVQLHPDIPIEKVGFVYNGILSKEMHICPEKHLGPLEICCVGSVSKRKGQDMIVEA
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHCCHHHHHHH
LVKMSPVQREKVHFTIVGDGTLRGELEKLCFEKGISKYIDFIGVSNQVENYLLRSDIFML
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEE
PSRDEGFPISILEAMRAGLPIISTNIAGIPEMVFSGINGIVISPCLEDIYDILCHIESYN
CCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCC
WSAMGKLSYELYQQKFTLDSMIESYANILNAI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVDIIHGGWLKAPNGASSVLRTLAESTSKFEEYGIQLSVYSMDLIVLKSFENIAAINQ
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHH
RKGLRYFIKKNANANSILAFISIYAIYLRHARKIVKYYFDQGLEESRILFIHDIFTCYYY
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
LKYRSRRQKTVLVLHNNGDTFNMLRQYYPVLNKSRLYSILLYIERKVLSEVDKVLFVAEN
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEEECC
PKDTFVQLHPDIPIEKVGFVYNGILSKEMHICPEKHLGPLEICCVGSVSKRKGQDMIVEA
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHCCHHHHHHH
LVKMSPVQREKVHFTIVGDGTLRGELEKLCFEKGISKYIDFIGVSNQVENYLLRSDIFML
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEE
PSRDEGFPISILEAMRAGLPIISTNIAGIPEMVFSGINGIVISPCLEDIYDILCHIESYN
CCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCC
WSAMGKLSYELYQQKFTLDSMIESYANILNAI
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]