Click here to switch to the map view.

The map label for this gene is ispD [H]

Identifier: 29347061

GI number: 29347061

Start: 2040648

End: 2042009

Strand: Reverse

Name: ispD [H]

Synonym: BT_1651

Alternate gene names: 29347061

Gene position: 2042009-2040648 (Counterclockwise)

Preceding gene: 29347062

Following gene: 29347060

Centisome position: 32.62

GC content: 34.65

Gene sequence:

>1362_bases
ATGAGAAATATAGCAGTAATTCTAGCCGGTGGTTCCGGACAACGATTGGGTGAGAATATACCTAAACAGTTTTTAAAAAT
AGCAGGTAAAAAAGTCATTGAACATACTATTACTGTTTTTCAAAATCATTCTTTGATAGATGAAATTGTAGTTGTAGTCC
ATCCAGACTATATAAGGGATGTAGAAGATATTTCGTTGAGGAATTCTTTCAATAAACTAAAAAAAATATTATTAGGGGGA
AAGGAAAGATACCACTCTAGTTTGGCAGCTATCAATGCTTATGATGAAGAAGTGAACTTATTATTTCACGATTCAGTTCG
TCCTCTTGTCAATGAGCGGATTATAAACGATTGCATCCGTGCATTGTTGACTTACAATGCCGTTGATGTAGCAATTCCTA
CAACGGATACTATTATTCAAGTAGATGATAATAATGAGATTGTCAAAATACCCTCACGCATATTATTGCGTAATGGACAA
ACACCTCAGGCATTTAAACGTTCTGTTATAAAAGCCGCTTATGAAAAAGCTTTGCTTGATCCCTTGTTTAAAACAACTGA
TGACTGTGGTGTAGTTTTAAAATATTTGCCTGATGAATCTATTTATGTAGTCAATGGAGAGCAATTTAATATGAAGTTGA
CATATAAAGAAGATATTTTTCTTTTAGATAAACTTTTTCAATTGCGTAGCATTAGGAACCAGCCCACTTTATTGGAAAAA
TCGGAAATGGAGGAACTACAAGGAAAGGTAGTTGTAGTTTTTGGGGGTACGTATGGTATCGGACAAGATATTTGTGAAAT
TTGTCGGGAATATAAAACGTCGTTGTATGTATACAGTCGTTCTACAGGAGTGGATGTGTCGAATAGTCAAAGTGTAAGGA
GAGTTTTATCTGAAATATTTGATAAGGAAGGACGGATTGACTGGATCGTTAATACCGCTGGTATATTGGACAAACAGCCT
TTGGCGAATATGAGTTATGAAGCGGTACATCAAAGCATTGATGTAAATTATTTAGGTAATATTATTGTTGCAAAAGAATC
TTTTTTTTATTTGAAAGAAAGTAAAGGATCGTTGTTGTTTTTTACTTCTAGCTCTTATACACGCGGAAGGGCGTTGTATA
GTTTATATTCATCGTCTAAAGCTGCTATTGTAAACTTTGTACAAGCTTTGAGTGAAGAGTGGTTTGAATATGGTATTCGT
GTGAATTGCATCAACCCAGAACGTACTAAGACCCCTATGCGTATTCAGAATTTTGGAAATGAACCGGAAGATACTTTATT
AGAACCGAAAGTGGTAGCAATCGTATCAGTAAATACTTTGCTATCGGAAATGACAGGTGAGGTTGTTGATGTAAAAAGAT
AA

Upstream 100 bases:

>100_bases
TGTGGTCTATTATTTGGTAGTTTCCAAGATATTCAAGTTTGAGGAGATGTGTTTTTTGCAAAATATGATTGTGAGATTGA
AAAATAAGTAATATTTTATA

Downstream 100 bases:

>100_bases
AATTGTATGATGTTGGTTATCAAACACTTATTAGGATACTTTATTTTACTTGTATCTTATGTTTTTCCCCGTATTTCAAC
CATTTGGGTCTTTGGAGGAG

Product: pyrophosphorylase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MRNIAVILAGGSGQRLGENIPKQFLKIAGKKVIEHTITVFQNHSLIDEIVVVVHPDYIRDVEDISLRNSFNKLKKILLGG
KERYHSSLAAINAYDEEVNLLFHDSVRPLVNERIINDCIRALLTYNAVDVAIPTTDTIIQVDDNNEIVKIPSRILLRNGQ
TPQAFKRSVIKAAYEKALLDPLFKTTDDCGVVLKYLPDESIYVVNGEQFNMKLTYKEDIFLLDKLFQLRSIRNQPTLLEK
SEMEELQGKVVVVFGGTYGIGQDICEICREYKTSLYVYSRSTGVDVSNSQSVRRVLSEIFDKEGRIDWIVNTAGILDKQP
LANMSYEAVHQSIDVNYLGNIIVAKESFFYLKESKGSLLFFTSSSYTRGRALYSLYSSSKAAIVNFVQALSEEWFEYGIR
VNCINPERTKTPMRIQNFGNEPEDTLLEPKVVAIVSVNTLLSEMTGEVVDVKR

Sequences:

>Translated_453_residues
MRNIAVILAGGSGQRLGENIPKQFLKIAGKKVIEHTITVFQNHSLIDEIVVVVHPDYIRDVEDISLRNSFNKLKKILLGG
KERYHSSLAAINAYDEEVNLLFHDSVRPLVNERIINDCIRALLTYNAVDVAIPTTDTIIQVDDNNEIVKIPSRILLRNGQ
TPQAFKRSVIKAAYEKALLDPLFKTTDDCGVVLKYLPDESIYVVNGEQFNMKLTYKEDIFLLDKLFQLRSIRNQPTLLEK
SEMEELQGKVVVVFGGTYGIGQDICEICREYKTSLYVYSRSTGVDVSNSQSVRRVLSEIFDKEGRIDWIVNTAGILDKQP
LANMSYEAVHQSIDVNYLGNIIVAKESFFYLKESKGSLLFFTSSSYTRGRALYSLYSSSKAAIVNFVQALSEEWFEYGIR
VNCINPERTKTPMRIQNFGNEPEDTLLEPKVVAIVSVNTLLSEMTGEVVDVKR
>Mature_453_residues
MRNIAVILAGGSGQRLGENIPKQFLKIAGKKVIEHTITVFQNHSLIDEIVVVVHPDYIRDVEDISLRNSFNKLKKILLGG
KERYHSSLAAINAYDEEVNLLFHDSVRPLVNERIINDCIRALLTYNAVDVAIPTTDTIIQVDDNNEIVKIPSRILLRNGQ
TPQAFKRSVIKAAYEKALLDPLFKTTDDCGVVLKYLPDESIYVVNGEQFNMKLTYKEDIFLLDKLFQLRSIRNQPTLLEK
SEMEELQGKVVVVFGGTYGIGQDICEICREYKTSLYVYSRSTGVDVSNSQSVRRVLSEIFDKEGRIDWIVNTAGILDKQP
LANMSYEAVHQSIDVNYLGNIIVAKESFFYLKESKGSLLFFTSSSYTRGRALYSLYSSSKAAIVNFVQALSEEWFEYGIR
VNCINPERTKTPMRIQNFGNEPEDTLLEPKVVAIVSVNTLLSEMTGEVVDVKR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=234, Percent_Identity=26.4957264957265, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI40254992, Length=183, Percent_Identity=27.3224043715847, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI59889578, Length=131, Percent_Identity=31.2977099236641, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1789104, Length=238, Percent_Identity=27.3109243697479, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787526, Length=216, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082100, Length=214, Percent_Identity=29.4392523364486, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=201, Percent_Identity=26.865671641791, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324126, Length=123, Percent_Identity=32.520325203252, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI23397609, Length=178, Percent_Identity=29.2134831460674, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 51246; Mature: 51246

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIAVILAGGSGQRLGENIPKQFLKIAGKKVIEHTITVFQNHSLIDEIVVVVHPDYIRD
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCHHHCC
VEDISLRNSFNKLKKILLGGKERYHSSLAAINAYDEEVNLLFHDSVRPLVNERIINDCIR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHH
ALLTYNAVDVAIPTTDTIIQVDDNNEIVKIPSRILLRNGQTPQAFKRSVIKAAYEKALLD
HHHHCCCEEEEECCCCEEEEECCCCCEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
PLFKTTDDCGVVLKYLPDESIYVVNGEQFNMKLTYKEDIFLLDKLFQLRSIRNQPTLLEK
HHHHCCCCCCHHEEECCCCCEEEEECCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCH
SEMEELQGKVVVVFGGTYGIGQDICEICREYKTSLYVYSRSTGVDVSNSQSVRRVLSEIF
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHH
DKEGRIDWIVNTAGILDKQPLANMSYEAVHQSIDVNYLGNIIVAKESFFYLKESKGSLLF
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHCCEEEEECCEEEEEECCCCEEE
FTSSSYTRGRALYSLYSSSKAAIVNFVQALSEEWFEYGIRVNCINPERTKTPMRIQNFGN
EECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCC
EPEDTLLEPKVVAIVSVNTLLSEMTGEVVDVKR
CCHHHCCCCCEEEEEEHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MRNIAVILAGGSGQRLGENIPKQFLKIAGKKVIEHTITVFQNHSLIDEIVVVVHPDYIRD
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCHHHCC
VEDISLRNSFNKLKKILLGGKERYHSSLAAINAYDEEVNLLFHDSVRPLVNERIINDCIR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHH
ALLTYNAVDVAIPTTDTIIQVDDNNEIVKIPSRILLRNGQTPQAFKRSVIKAAYEKALLD
HHHHCCCEEEEECCCCEEEEECCCCCEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
PLFKTTDDCGVVLKYLPDESIYVVNGEQFNMKLTYKEDIFLLDKLFQLRSIRNQPTLLEK
HHHHCCCCCCHHEEECCCCCEEEEECCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCH
SEMEELQGKVVVVFGGTYGIGQDICEICREYKTSLYVYSRSTGVDVSNSQSVRRVLSEIF
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHH
DKEGRIDWIVNTAGILDKQPLANMSYEAVHQSIDVNYLGNIIVAKESFFYLKESKGSLLF
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHCCEEEEECCEEEEEECCCCEEE
FTSSSYTRGRALYSLYSSSKAAIVNFVQALSEEWFEYGIRVNCINPERTKTPMRIQNFGN
EECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCC
EPEDTLLEPKVVAIVSVNTLLSEMTGEVVDVKR
CCHHHCCCCCEEEEEEHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA