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The map label for this gene is wcaJ [H]

Identifier: 29347054

GI number: 29347054

Start: 2034033

End: 2034659

Strand: Reverse

Name: wcaJ [H]

Synonym: BT_1644

Alternate gene names: 29347054

Gene position: 2034659-2034033 (Counterclockwise)

Preceding gene: 29347055

Following gene: 29347053

Centisome position: 32.5

GC content: 39.55

Gene sequence:

>627_bases
ATGGAACAACAAACAACTTTCATTCCTGACGGAATGAATGCTTTTGAGCGCAATGTAAAACGGGTGGGGGATTGCATGAT
TGCGGGAATATTAATGATTATTTTTTCTCCATTATTTTTAGCCTGCTATATAGCTGTGAAACGGGAAGATGGTGGTCCGG
CTATCTTCAGGCAGGAACGTATCGGACGTTTCGGACGTCCGTTTTATATTTATAAGTTCCGTAGTATGCGTTTGGATGCG
GAAAAAATGGGTCCTGCGCTATATAAGGGTGGGAAAGATGAACGATTGACTAAAGTCGGTAAGTTTTTGCGTGAACATCA
TTTAGATGAATTGCCGCAACTTTGGAATGTCTTTTGTGGACATATGGCTTTTATTGGTCCTCGTCCCGAACGGAAATATT
TCATAGATCAGATAATGGAACACGATGCTCGTTACGTATTTTTGTATCAGATACGACCGGGCGTAACTTCGTATGCTACG
CTATACAATGGGTACACAGATACGATGGATAAAATGTTACGTCGTCTCCGATATGATTTGTTTTATTTGCAACATCGCTC
ATGGTGGTTTGATTTTAAGATTTTGGTGAAGACATTTATAAATATTTGTTTTGGCAAGAAGTTTTGA

Upstream 100 bases:

>100_bases
ATACGGGTGATTACCAGGATGATGCCCAATTCTTTACGGGCTATCTTGTATAAAAAAGCTTTGAGAAAATAATCTTTAAC
CAAACTATAATTAATAATTT

Downstream 100 bases:

>100_bases
GTAGTGAAATTTGAGACTTTTATCTCTTTGATTTTAGTAGATAAAAAAGTTGTTTCAACAGAAGATGAGTTGAATATTAC
TGATCCTCCTTATAAAATAG

Product: putative CPS biosynthesis glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MEQQTTFIPDGMNAFERNVKRVGDCMIAGILMIIFSPLFLACYIAVKREDGGPAIFRQERIGRFGRPFYIYKFRSMRLDA
EKMGPALYKGGKDERLTKVGKFLREHHLDELPQLWNVFCGHMAFIGPRPERKYFIDQIMEHDARYVFLYQIRPGVTSYAT
LYNGYTDTMDKMLRRLRYDLFYLQHRSWWFDFKILVKTFINICFGKKF

Sequences:

>Translated_208_residues
MEQQTTFIPDGMNAFERNVKRVGDCMIAGILMIIFSPLFLACYIAVKREDGGPAIFRQERIGRFGRPFYIYKFRSMRLDA
EKMGPALYKGGKDERLTKVGKFLREHHLDELPQLWNVFCGHMAFIGPRPERKYFIDQIMEHDARYVFLYQIRPGVTSYAT
LYNGYTDTMDKMLRRLRYDLFYLQHRSWWFDFKILVKTFINICFGKKF
>Mature_208_residues
MEQQTTFIPDGMNAFERNVKRVGDCMIAGILMIIFSPLFLACYIAVKREDGGPAIFRQERIGRFGRPFYIYKFRSMRLDA
EKMGPALYKGGKDERLTKVGKFLREHHLDELPQLWNVFCGHMAFIGPRPERKYFIDQIMEHDARYVFLYQIRPGVTSYAT
LYNGYTDTMDKMLRRLRYDLFYLQHRSWWFDFKILVKTFINICFGKKF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=196, Percent_Identity=37.7551020408163, Blast_Score=141, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 24825; Mature: 24825

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQTTFIPDGMNAFERNVKRVGDCMIAGILMIIFSPLFLACYIAVKREDGGPAIFRQER
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHH
IGRFGRPFYIYKFRSMRLDAEKMGPALYKGGKDERLTKVGKFLREHHLDELPQLWNVFCG
HCCCCCCEEEEEHHHHCCCHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMAFIGPRPERKYFIDQIMEHDARYVFLYQIRPGVTSYATLYNGYTDTMDKMLRRLRYDL
HHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHH
FYLQHRSWWFDFKILVKTFINICFGKKF
HHHHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQQTTFIPDGMNAFERNVKRVGDCMIAGILMIIFSPLFLACYIAVKREDGGPAIFRQER
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHH
IGRFGRPFYIYKFRSMRLDAEKMGPALYKGGKDERLTKVGKFLREHHLDELPQLWNVFCG
HCCCCCCEEEEEHHHHCCCHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMAFIGPRPERKYFIDQIMEHDARYVFLYQIRPGVTSYATLYNGYTDTMDKMLRRLRYDL
HHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHH
FYLQHRSWWFDFKILVKTFINICFGKKF
HHHHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]