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The map label for this gene is nrfA

Identifier: 29346827

GI number: 29346827

Start: 1750325

End: 1751806

Strand: Reverse

Name: nrfA

Synonym: BT_1417

Alternate gene names: 29346827

Gene position: 1751806-1750325 (Counterclockwise)

Preceding gene: 29346828

Following gene: 29346826

Centisome position: 27.98

GC content: 46.42

Gene sequence:

>1482_bases
ATGGAAAAGAAATTAAAATCATGGCAAGGATGGCTGTTGTCCGGAGGTTCAATGGTCGTTGTTTTTGTTTTAGGATTATG
TGTTTCTGCGTTGATGGAGAGGAGGGCGGAAGTCGCCAGTATCTTTAATAACCGCAAAACTGTCATTAAAGGTATTGAAG
CACGCAACGAACTGTTTAAAGATGATTTTCCCCGCGAATATCAGACTTGGACGGAAACGGCAAAGACTGATTTTGAAAGC
GAGTTTAATGGCAATGTCGCTGTTGATGCTTTGGAAAAACGTCCGGAAATGGTTATTCTGTGGGCAGGATATGCATTTTC
AAAAGACTATTCCACTCCGCGCGGACATATGCATGCTATCGAGGATATTACGGCGTCACTTCGTACCGGTTCTCCTGCAG
GTCCGCACGATGGCCCTCAGCCTTCTACTTGCTGGACTTGTAAGAGTCCGGATGTTCCCCGCATGATGGAAGCATTGGGA
GTCGATTCTTTCTATAATAACAAATGGGCTGCCTTTGGTGATGAAATCGTGAATCCGATCGGCTGTTCCGACTGCCACGA
TCCGGAAACAATGAATCTTCATATCAGCCGTCCGGCATTGATTGAAGCTTTCCAGCGTCAGGGAAAAGATATCACAAAAG
CTACTCCACAGGAAATGCGTTCATTGGTCTGTGCGCAGTGCCACGTAGAATATTACTTTAAGGGTGACGGTAAATATTTG
ACTTTCCCGTGGGATAAAGGTTTCACTGTAGAAGACATGGAAGCATATTATGATGAGGCAGGTTTCTATGATTACATTCA
TAAGTTAAGCCGTACTCCGATCTTGAAAGCCCAGCATCCCGACTATGAAATAGCTCAGATGGGTATTCACGGGCAAAGGG
GCGTTTCTTGTGCCGATTGTCATATGCCTTATAAGAGTGAAGGGGGCGTGAAATTCAGTGACCATCATATTCAGAGCCCA
TTGGCAATGATTGACCGCACCTGTCAGACTTGTCATCGTGAGAGTGAAGAAACATTACGTAACAATGTGTACGAGCGTCA
GCGCAAGGCAAATGAAATCCGTAACCGTCTGGAACAGGAACTGGCAAAGGCGCATATCGAAGCAAAATTTGCCTGGGATA
AGGGTGCTACAGAAGATCAGATGAAAGATGTGCTTGCATTGATTCGTCAGGCACAATGGCGTTGGGACTTCGGTGTGGCA
TCACATGGAGGTTCTTTCCATGCACCGCAGGAGATTCAGAGAATTCTTTCTCATGGTCTGGACCGTGCTATGCAGGCTCG
TCTGGCTGTATCCAAAGTTTTAGCGAAGCATGGGTATACGGAAGACGTTCCGATGCCGGACATTTCAACGAAGGCCAAAG
CACAGGAATATATCGGTCTTGATATGGATGCTGAAAGAGCTGCTAAGGAGAAATTCCTGAAAACGACCGTTCCGGCATGG
CTGGAGAAGGCAAAGGCAAACGGGCGTCTGGCACAAAAGTAA

Upstream 100 bases:

>100_bases
CTTCCCGAATCGCCTGTACCGGAATGGCTCCGGAAGATGGTTAATAATAAGGAATAAGCATATACATAACTACGTAATAA
AATAATAAAACCGATATACA

Downstream 100 bases:

>100_bases
AATAGGGATATATGTGGAGCAAACCGTGGAGTTATAAAGAAGGGCTGGTGATTGGTGCGGGATTACTGGTGATAGGTATT
TTGCTGCAAATGGCAGTGGG

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MEKKLKSWQGWLLSGGSMVVVFVLGLCVSALMERRAEVASIFNNRKTVIKGIEARNELFKDDFPREYQTWTETAKTDFES
EFNGNVAVDALEKRPEMVILWAGYAFSKDYSTPRGHMHAIEDITASLRTGSPAGPHDGPQPSTCWTCKSPDVPRMMEALG
VDSFYNNKWAAFGDEIVNPIGCSDCHDPETMNLHISRPALIEAFQRQGKDITKATPQEMRSLVCAQCHVEYYFKGDGKYL
TFPWDKGFTVEDMEAYYDEAGFYDYIHKLSRTPILKAQHPDYEIAQMGIHGQRGVSCADCHMPYKSEGGVKFSDHHIQSP
LAMIDRTCQTCHRESEETLRNNVYERQRKANEIRNRLEQELAKAHIEAKFAWDKGATEDQMKDVLALIRQAQWRWDFGVA
SHGGSFHAPQEIQRILSHGLDRAMQARLAVSKVLAKHGYTEDVPMPDISTKAKAQEYIGLDMDAERAAKEKFLKTTVPAW
LEKAKANGRLAQK

Sequences:

>Translated_493_residues
MEKKLKSWQGWLLSGGSMVVVFVLGLCVSALMERRAEVASIFNNRKTVIKGIEARNELFKDDFPREYQTWTETAKTDFES
EFNGNVAVDALEKRPEMVILWAGYAFSKDYSTPRGHMHAIEDITASLRTGSPAGPHDGPQPSTCWTCKSPDVPRMMEALG
VDSFYNNKWAAFGDEIVNPIGCSDCHDPETMNLHISRPALIEAFQRQGKDITKATPQEMRSLVCAQCHVEYYFKGDGKYL
TFPWDKGFTVEDMEAYYDEAGFYDYIHKLSRTPILKAQHPDYEIAQMGIHGQRGVSCADCHMPYKSEGGVKFSDHHIQSP
LAMIDRTCQTCHRESEETLRNNVYERQRKANEIRNRLEQELAKAHIEAKFAWDKGATEDQMKDVLALIRQAQWRWDFGVA
SHGGSFHAPQEIQRILSHGLDRAMQARLAVSKVLAKHGYTEDVPMPDISTKAKAQEYIGLDMDAERAAKEKFLKTTVPAW
LEKAKANGRLAQK
>Mature_493_residues
MEKKLKSWQGWLLSGGSMVVVFVLGLCVSALMERRAEVASIFNNRKTVIKGIEARNELFKDDFPREYQTWTETAKTDFES
EFNGNVAVDALEKRPEMVILWAGYAFSKDYSTPRGHMHAIEDITASLRTGSPAGPHDGPQPSTCWTCKSPDVPRMMEALG
VDSFYNNKWAAFGDEIVNPIGCSDCHDPETMNLHISRPALIEAFQRQGKDITKATPQEMRSLVCAQCHVEYYFKGDGKYL
TFPWDKGFTVEDMEAYYDEAGFYDYIHKLSRTPILKAQHPDYEIAQMGIHGQRGVSCADCHMPYKSEGGVKFSDHHIQSP
LAMIDRTCQTCHRESEETLRNNVYERQRKANEIRNRLEQELAKAHIEAKFAWDKGATEDQMKDVLALIRQAQWRWDFGVA
SHGGSFHAPQEIQRILSHGLDRAMQARLAVSKVLAKHGYTEDVPMPDISTKAKAQEYIGLDMDAERAAKEKFLKTTVPAW
LEKAKANGRLAQK

Specific function: Plays a role in nitrite reduction

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family

Homologues:

Organism=Escherichia coli, GI1790506, Length=449, Percent_Identity=46.3251670378619, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFA_BACTN (Q8A7V7)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_810330.1
- HSSP:   Q9S1E5
- ProteinModelPortal:   Q8A7V7
- SMR:   Q8A7V7
- GeneID:   1071634
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_1417
- NMPDR:   fig|226186.1.peg.1417
- HOGENOM:   HBG488281
- OMA:   PWDMGTT
- PhylomeDB:   Q8A7V7
- ProtClustDB:   PRK11125
- BioCyc:   BTHE226186:BT_1417-MONOMER
- BRENDA:   1.7.2.2
- GO:   GO:0006810
- HAMAP:   MF_01182
- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031
- PIRSF:   PIRSF000243

Pfam domain/function: PF02335 Cytochrom_C552

EC number: =1.7.2.2

Molecular weight: Translated: 55911; Mature: 55911

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 144-144 BINDING 147-147 BINDING 182-182 BINDING 185-185 BINDING 224-224 BINDING 227-227 BINDING 231-231 BINDING 279-279 BINDING 297-297 BINDING 300-300 BINDING 328-328 BINDING 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKLKSWQGWLLSGGSMVVVFVLGLCVSALMERRAEVASIFNNRKTVIKGIEARNELFK
CCCHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDFPREYQTWTETAKTDFESEFNGNVAVDALEKRPEMVILWAGYAFSKDYSTPRGHMHAI
CCCCHHHHHHHHHHHHCCHHHCCCCEEEEEHHCCCCEEEEEECCEECCCCCCCCCHHHHH
EDITASLRTGSPAGPHDGPQPSTCWTCKSPDVPRMMEALGVDSFYNNKWAAFGDEIVNPI
HHHHHHHHCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCHHHCCCHHHHHHHHCCCC
GCSDCHDPETMNLHISRPALIEAFQRQGKDITKATPQEMRSLVCAQCHVEYYFKGDGKYL
CCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCEE
TFPWDKGFTVEDMEAYYDEAGFYDYIHKLSRTPILKAQHPDYEIAQMGIHGQRGVSCADC
EECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCCCHHHC
HMPYKSEGGVKFSDHHIQSPLAMIDRTCQTCHRESEETLRNNVYERQRKANEIRNRLEQE
CCCCCCCCCCEECHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKAHIEAKFAWDKGATEDQMKDVLALIRQAQWRWDFGVASHGGSFHAPQEIQRILSHGL
HHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
DRAMQARLAVSKVLAKHGYTEDVPMPDISTKAKAQEYIGLDMDAERAAKEKFLKTTVPAW
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LEKAKANGRLAQK
HHHHHCCCCCCCC
>Mature Secondary Structure
MEKKLKSWQGWLLSGGSMVVVFVLGLCVSALMERRAEVASIFNNRKTVIKGIEARNELFK
CCCHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDFPREYQTWTETAKTDFESEFNGNVAVDALEKRPEMVILWAGYAFSKDYSTPRGHMHAI
CCCCHHHHHHHHHHHHCCHHHCCCCEEEEEHHCCCCEEEEEECCEECCCCCCCCCHHHHH
EDITASLRTGSPAGPHDGPQPSTCWTCKSPDVPRMMEALGVDSFYNNKWAAFGDEIVNPI
HHHHHHHHCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCHHHCCCHHHHHHHHCCCC
GCSDCHDPETMNLHISRPALIEAFQRQGKDITKATPQEMRSLVCAQCHVEYYFKGDGKYL
CCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCEE
TFPWDKGFTVEDMEAYYDEAGFYDYIHKLSRTPILKAQHPDYEIAQMGIHGQRGVSCADC
EECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCCCHHHC
HMPYKSEGGVKFSDHHIQSPLAMIDRTCQTCHRESEETLRNNVYERQRKANEIRNRLEQE
CCCCCCCCCCEECHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKAHIEAKFAWDKGATEDQMKDVLALIRQAQWRWDFGVASHGGSFHAPQEIQRILSHGL
HHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
DRAMQARLAVSKVLAKHGYTEDVPMPDISTKAKAQEYIGLDMDAERAAKEKFLKTTVPAW
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LEKAKANGRLAQK
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928