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The map label for this gene is proS [H]

Identifier: 29346339

GI number: 29346339

Start: 1142831

End: 1144324

Strand: Reverse

Name: proS [H]

Synonym: BT_0929

Alternate gene names: 29346339

Gene position: 1144324-1142831 (Counterclockwise)

Preceding gene: 29346341

Following gene: 29346338

Centisome position: 18.28

GC content: 43.64

Gene sequence:

>1494_bases
ATGGCAAAAGAACTGAAAGACCTCACCAAACGTAGCGAAAACTACTCACAGTGGTACAACGATTTGGTGGTAAAAGCTGA
TTTGGCAGAACAGTCTGCCGTACGCGGATGTATGGTGATTAAGCCTTACGGATACGCAATTTGGGAAAAGATGCAACGTC
AACTAGACGATATGTTCAAGGAAACAGGACACGTGAATGCTTATTTCCCATTATTAATTCCAAAATCATTCCTGAGCCGC
GAAGCAGAACACGTAGAAGGTTTCGCTAAAGAATGTGCCGTAGTGACTCATTACCGTTTGAAAAATGCAGAAGACGGCTC
CGGCGTAGTAGTAGACCCTGCTGCCAAATTGGAAGAGGAACTGATTATTCGCCCTACATCAGAAACAATCATCTGGAATA
CATATAAAAACTGGATTCAGTCTTATCGTGACCTGCCTATTCTTTGTAATCAGTGGGCAAACGTTTTCCGTTGGGAAATG
CGTACCCGTCTTTTCCTGCGTACTGCCGAATTCCTGTGGCAGGAAGGACACACCGCTCATGCCACACGCGAAGAAGCAGA
GGAAGAAGCGATCAGAATGCTGAATGTATACGGCGAATTTGCTGAGAAATACATGGCTGTTCCGGTAGTGAAAGGTGTAA
AATCCGCTAACGAACGTTTTGCCGGTGCACTTGATACCTATACCATCGAAGCTATGATGCAGGATGGAAAAGCATTGCAA
AGCGGTACATCTCATTTCCTTGGACAGAACTTTGCCAAGGCATTTGATGTTCAGTTTGTGAATAAGGAAAATAAGATGGA
ATATGTATGGGCAACTTCATGGGGTGTTTCTACCCGTTTGATGGGTGCTCTGATTATGACTCATTCCGATGATAACGGTC
TGGTATTACCTCCACATCTGGCTCCGATTCAGGTAGTAATCGTTCCTATCTATAAGAACGACGAGCAACTGAAACAAATC
GACGCGAAAGTGGAAGGCATTGTTGCCAAACTGAAAGCACTGGGCATTTCTGTGAAATATGACAATGCGGACAACAAACG
TCCGGGCTTCAAATTCGCAGATTATGAATTGAAAGGTGTCCCTGTTCGTCTGGTGATGGGAGGACGTGACCTCGAAAACA
ATACAATGGAAGTAATGCGCCGCGATACACTGGAAAAAGAAACTGTGACTTGCGATGGCATCGAAACATATGTTCAGAAA
CTGCTCGAAGAAATTCAAGCAAATATCTACAAAAAAGCATTGGATTACCGCAATTCTAAAATCACGACAGTAGATACTTA
CGAAGAATTTAAAGAGAAAATCGAAGAAGGCGGCTTTATCCTAGCTCATTGGGACGGAACAACCGAGACTGAAGAAAAGA
TTAAAGAGGATACAAAAGCAACGATTCGTTGTATCCCATTCGATTCGTACGTTGAAGGTGATAAAGAACCGGGAAAATGT
ATGGTAACGGGCAAACCTTCTGCCTGCCGCGTCGTTTTTGCACGTTCTTATTAA

Upstream 100 bases:

>100_bases
ATATAAAGAAAATAATGCGAGAAGAATAGGGCAGTTCGTTCCTTTTTCGTATTTTTGCACCCTGTTTTAACATCCAAGAA
AACTTAAATAGAAAAATATA

Downstream 100 bases:

>100_bases
AAATATCTATTTCAATAGTAAAAGAGTCAGATTTTTAAAATTGAGAATCTGACTCTTTTTTTATCCATTTCAGTTTTATT
TCAGAAATATCTCTTTTCTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MAKELKDLTKRSENYSQWYNDLVVKADLAEQSAVRGCMVIKPYGYAIWEKMQRQLDDMFKETGHVNAYFPLLIPKSFLSR
EAEHVEGFAKECAVVTHYRLKNAEDGSGVVVDPAAKLEEELIIRPTSETIIWNTYKNWIQSYRDLPILCNQWANVFRWEM
RTRLFLRTAEFLWQEGHTAHATREEAEEEAIRMLNVYGEFAEKYMAVPVVKGVKSANERFAGALDTYTIEAMMQDGKALQ
SGTSHFLGQNFAKAFDVQFVNKENKMEYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHLAPIQVVIVPIYKNDEQLKQI
DAKVEGIVAKLKALGISVKYDNADNKRPGFKFADYELKGVPVRLVMGGRDLENNTMEVMRRDTLEKETVTCDGIETYVQK
LLEEIQANIYKKALDYRNSKITTVDTYEEFKEKIEEGGFILAHWDGTTETEEKIKEDTKATIRCIPFDSYVEGDKEPGKC
MVTGKPSACRVVFARSY

Sequences:

>Translated_497_residues
MAKELKDLTKRSENYSQWYNDLVVKADLAEQSAVRGCMVIKPYGYAIWEKMQRQLDDMFKETGHVNAYFPLLIPKSFLSR
EAEHVEGFAKECAVVTHYRLKNAEDGSGVVVDPAAKLEEELIIRPTSETIIWNTYKNWIQSYRDLPILCNQWANVFRWEM
RTRLFLRTAEFLWQEGHTAHATREEAEEEAIRMLNVYGEFAEKYMAVPVVKGVKSANERFAGALDTYTIEAMMQDGKALQ
SGTSHFLGQNFAKAFDVQFVNKENKMEYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHLAPIQVVIVPIYKNDEQLKQI
DAKVEGIVAKLKALGISVKYDNADNKRPGFKFADYELKGVPVRLVMGGRDLENNTMEVMRRDTLEKETVTCDGIETYVQK
LLEEIQANIYKKALDYRNSKITTVDTYEEFKEKIEEGGFILAHWDGTTETEEKIKEDTKATIRCIPFDSYVEGDKEPGKC
MVTGKPSACRVVFARSY
>Mature_496_residues
AKELKDLTKRSENYSQWYNDLVVKADLAEQSAVRGCMVIKPYGYAIWEKMQRQLDDMFKETGHVNAYFPLLIPKSFLSRE
AEHVEGFAKECAVVTHYRLKNAEDGSGVVVDPAAKLEEELIIRPTSETIIWNTYKNWIQSYRDLPILCNQWANVFRWEMR
TRLFLRTAEFLWQEGHTAHATREEAEEEAIRMLNVYGEFAEKYMAVPVVKGVKSANERFAGALDTYTIEAMMQDGKALQS
GTSHFLGQNFAKAFDVQFVNKENKMEYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHLAPIQVVIVPIYKNDEQLKQID
AKVEGIVAKLKALGISVKYDNADNKRPGFKFADYELKGVPVRLVMGGRDLENNTMEVMRRDTLEKETVTCDGIETYVQKL
LEEIQANIYKKALDYRNSKITTVDTYEEFKEKIEEGGFILAHWDGTTETEEKIKEDTKATIRCIPFDSYVEGDKEPGKCM
VTGKPSACRVVFARSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=510, Percent_Identity=39.2156862745098, Blast_Score=367, Evalue=1e-101,
Organism=Escherichia coli, GI1786392, Length=165, Percent_Identity=29.6969696969697, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71991342, Length=506, Percent_Identity=39.7233201581028, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71991346, Length=371, Percent_Identity=43.9353099730458, Blast_Score=326, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6321808, Length=523, Percent_Identity=38.4321223709369, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24649466, Length=511, Percent_Identity=37.9647749510763, Blast_Score=346, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24649468, Length=511, Percent_Identity=37.9647749510763, Blast_Score=346, Evalue=3e-95,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 56848; Mature: 56717

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKELKDLTKRSENYSQWYNDLVVKADLAEQSAVRGCMVIKPYGYAIWEKMQRQLDDMFK
CCHHHHHHHHCCCHHHHHHHHHEEEECHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHH
ETGHVNAYFPLLIPKSFLSREAEHVEGFAKECAVVTHYRLKNAEDGSGVVVDPAAKLEEE
HCCCCCEEEHHCCCHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCHHHCCCC
LIIRPTSETIIWNTYKNWIQSYRDLPILCNQWANVFRWEMRTRLFLRTAEFLWQEGHTAH
EEEECCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ATREEAEEEAIRMLNVYGEFAEKYMAVPVVKGVKSANERFAGALDTYTIEAMMQDGKALQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHH
SGTSHFLGQNFAKAFDVQFVNKENKMEYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHL
HCHHHHHHHHHHHHHCEEEECCCCCEEEEEEECCCHHHHHHHEEEEEECCCCCEEECCCC
APIQVVIVPIYKNDEQLKQIDAKVEGIVAKLKALGISVKYDNADNKRPGFKFADYELKGV
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEECEEECCC
PVRLVMGGRDLENNTMEVMRRDTLEKETVTCDGIETYVQKLLEEIQANIYKKALDYRNSK
CEEEEECCCCCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ITTVDTYEEFKEKIEEGGFILAHWDGTTETEEKIKEDTKATIRCIPFDSYVEGDKEPGKC
EEEEHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCEEEEECCHHHHCCCCCCCEE
MVTGKPSACRVVFARSY
EEECCCCCEEEEEEECC
>Mature Secondary Structure 
AKELKDLTKRSENYSQWYNDLVVKADLAEQSAVRGCMVIKPYGYAIWEKMQRQLDDMFK
CHHHHHHHHCCCHHHHHHHHHEEEECHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHH
ETGHVNAYFPLLIPKSFLSREAEHVEGFAKECAVVTHYRLKNAEDGSGVVVDPAAKLEEE
HCCCCCEEEHHCCCHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCHHHCCCC
LIIRPTSETIIWNTYKNWIQSYRDLPILCNQWANVFRWEMRTRLFLRTAEFLWQEGHTAH
EEEECCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ATREEAEEEAIRMLNVYGEFAEKYMAVPVVKGVKSANERFAGALDTYTIEAMMQDGKALQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHH
SGTSHFLGQNFAKAFDVQFVNKENKMEYVWATSWGVSTRLMGALIMTHSDDNGLVLPPHL
HCHHHHHHHHHHHHHCEEEECCCCCEEEEEEECCCHHHHHHHEEEEEECCCCCEEECCCC
APIQVVIVPIYKNDEQLKQIDAKVEGIVAKLKALGISVKYDNADNKRPGFKFADYELKGV
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEECEEECCC
PVRLVMGGRDLENNTMEVMRRDTLEKETVTCDGIETYVQKLLEEIQANIYKKALDYRNSK
CEEEEECCCCCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ITTVDTYEEFKEKIEEGGFILAHWDGTTETEEKIKEDTKATIRCIPFDSYVEGDKEPGKC
EEEEHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCEEEEECCHHHHCCCCCCCEE
MVTGKPSACRVVFARSY
EEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA