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The map label for this gene is napF [H]

Identifier: 29346185

GI number: 29346185

Start: 965896

End: 967461

Strand: Reverse

Name: napF [H]

Synonym: BT_0775

Alternate gene names: 29346185

Gene position: 967461-965896 (Counterclockwise)

Preceding gene: 29346186

Following gene: 29346184

Centisome position: 15.45

GC content: 46.74

Gene sequence:

>1566_bases
ATGTTAAGAAAGATACGTATCGGAATATCAGTCGTCCTTTTTGCACTGATTACTTTTTATTTTCTCGATTTTGCTAATAT
TCTGCCGAACAGTTTTCATCGATTGGCACATATACAGTTTGTCCCTGCCGTGCTATCTCTCAGCATAGGCATACTGATTG
TACTGATTGCGTTAACCCTGTTGTTCGGACGTATCTATTGCTCTACCATCTGTCCGATGGGAATATGGCAGGATGTTATA
GCACGCATTTCAAAGTCTGTGGGAAAGAAGAAAAAAAGATATCGGTACAGTCCTGCAAAGAATATGCTGAGATGGACCGT
GCTCGGAGTGACTGTAATTGCCTCCGTATGCGGCTTCTCAGTGGTATTGGGATTGCTGGACCCGTATAGCGCATACGGAC
GCATTGTAGTACATATCTTCAAACCGGTGTATATGCTGGGAAACAACCTGCTGGAATCCGTATTTTCCAGATTCGACAAC
TACACGTTTTATCAGGTAGACACCTCGGTATTAAGCCTTTCTTCCCTTCTTATAGCGATACTGACCTTTGCCGCCATTCT
TATATTAGCATGGAAACATGGACGCACGTGGTGTAATACCATTTGTCCGGTAGGTACGGTATTAGGATTTTTGAGCCGTT
ACTCTTTGTTCAAAGTCCGTATCGATACCGCAAAATGTAATGGTTGCGGACTTTGCGCTACCAAATGCAAAGCTGCCTGC
ATCCACAGCAAAGAACACGCTATTGACTATAGCCGCTGTGTAGACTGTTTCGACTGTCTCGGTGCCTGCAAACAGAAAGC
GCTGGTTTACGCCCCTGCTTCAAAGGGACAACAACGTTCTTCCGCTACAGAAAAACAACCGGTGGCAACAGAATCATCTT
CCGTTGCTGCAGATTCTTCCAAACGTCGTTTTCTGCTTGCCGGACTGGCTACTGCCGGAGCAGCTCCCACGCTTCTTACA
AAGGCGCAAGAATCCATTGCGACGATGGAAGGCAAAAAGGCATACAAAAAAGAAAATCCGATTACTCCTCCGGGATCTGT
CAGCCAGAAGCGTTTCCAGCAACATTGTACATCATGCCATCTTTGTGTCAGCAAATGCCCGTCGCATGTACTGAAACCCG
CTTTCATGGAATACGGTCTGGGAGGTGTGATGCAGCCAACGGTCAGTTTCGAAAAAGGATTCTGTAATTTCGACTGTACC
GTATGCAGCGATGTCTGTCCCAACGGTGCCATCCCTCCGTTGACGGTAGAACAGAAACATCTCACTCAGATGGGCTATGT
CGTCTTTATAGAAGAAAATTGCATTGTCCTCACTGACGGAACCAGTTGCGGAGCCTGTTCAGAGCATTGTCCTACGCAAG
CCATTGCTATGGTACCCTACAAAGACGGACTGACTCTTCCCCACGTCAATACAGAGATCTGCGTCGGATGTGGCGGCTGC
GAATACGTCTGCCCTGCCCGCCCGTTCCGTGCCGTATATATCGAAGGAAACCCTGTGCAGAAAGAAGCGAAACCTTTCAA
AGAATCAGAAGAACATAAAGTTGAGATAGACGATTTCGGATTCTAG

Upstream 100 bases:

>100_bases
GCTGGACACCGTAGGTCACCTTGCCAAAGGAGAAGCACTGAAAGTAGGAACGATGGACATCGACAAACTGAATGTCAAGC
GAATTAAGATGTAAACCACC

Downstream 100 bases:

>100_bases
CAGCATCAGCCCCAAATATGGATAGCTTTCAGAGGATTGTATACAATTCAAAAAAACTATCCATTATATTCCTCCTACTT
TCAATAAATTCACTAAATTC

Product: ferredoxin-type protein

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MLRKIRIGISVVLFALITFYFLDFANILPNSFHRLAHIQFVPAVLSLSIGILIVLIALTLLFGRIYCSTICPMGIWQDVI
ARISKSVGKKKKRYRYSPAKNMLRWTVLGVTVIASVCGFSVVLGLLDPYSAYGRIVVHIFKPVYMLGNNLLESVFSRFDN
YTFYQVDTSVLSLSSLLIAILTFAAILILAWKHGRTWCNTICPVGTVLGFLSRYSLFKVRIDTAKCNGCGLCATKCKAAC
IHSKEHAIDYSRCVDCFDCLGACKQKALVYAPASKGQQRSSATEKQPVATESSSVAADSSKRRFLLAGLATAGAAPTLLT
KAQESIATMEGKKAYKKENPITPPGSVSQKRFQQHCTSCHLCVSKCPSHVLKPAFMEYGLGGVMQPTVSFEKGFCNFDCT
VCSDVCPNGAIPPLTVEQKHLTQMGYVVFIEENCIVLTDGTSCGACSEHCPTQAIAMVPYKDGLTLPHVNTEICVGCGGC
EYVCPARPFRAVYIEGNPVQKEAKPFKESEEHKVEIDDFGF

Sequences:

>Translated_521_residues
MLRKIRIGISVVLFALITFYFLDFANILPNSFHRLAHIQFVPAVLSLSIGILIVLIALTLLFGRIYCSTICPMGIWQDVI
ARISKSVGKKKKRYRYSPAKNMLRWTVLGVTVIASVCGFSVVLGLLDPYSAYGRIVVHIFKPVYMLGNNLLESVFSRFDN
YTFYQVDTSVLSLSSLLIAILTFAAILILAWKHGRTWCNTICPVGTVLGFLSRYSLFKVRIDTAKCNGCGLCATKCKAAC
IHSKEHAIDYSRCVDCFDCLGACKQKALVYAPASKGQQRSSATEKQPVATESSSVAADSSKRRFLLAGLATAGAAPTLLT
KAQESIATMEGKKAYKKENPITPPGSVSQKRFQQHCTSCHLCVSKCPSHVLKPAFMEYGLGGVMQPTVSFEKGFCNFDCT
VCSDVCPNGAIPPLTVEQKHLTQMGYVVFIEENCIVLTDGTSCGACSEHCPTQAIAMVPYKDGLTLPHVNTEICVGCGGC
EYVCPARPFRAVYIEGNPVQKEAKPFKESEEHKVEIDDFGF
>Mature_521_residues
MLRKIRIGISVVLFALITFYFLDFANILPNSFHRLAHIQFVPAVLSLSIGILIVLIALTLLFGRIYCSTICPMGIWQDVI
ARISKSVGKKKKRYRYSPAKNMLRWTVLGVTVIASVCGFSVVLGLLDPYSAYGRIVVHIFKPVYMLGNNLLESVFSRFDN
YTFYQVDTSVLSLSSLLIAILTFAAILILAWKHGRTWCNTICPVGTVLGFLSRYSLFKVRIDTAKCNGCGLCATKCKAAC
IHSKEHAIDYSRCVDCFDCLGACKQKALVYAPASKGQQRSSATEKQPVATESSSVAADSSKRRFLLAGLATAGAAPTLLT
KAQESIATMEGKKAYKKENPITPPGSVSQKRFQQHCTSCHLCVSKCPSHVLKPAFMEYGLGGVMQPTVSFEKGFCNFDCT
VCSDVCPNGAIPPLTVEQKHLTQMGYVVFIEENCIVLTDGTSCGACSEHCPTQAIAMVPYKDGLTLPHVNTEICVGCGGC
EYVCPARPFRAVYIEGNPVQKEAKPFKESEEHKVEIDDFGF

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=162, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788533, Length=209, Percent_Identity=30.622009569378, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787226, Length=228, Percent_Identity=23.6842105263158, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 57078; Mature: 57078

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKIRIGISVVLFALITFYFLDFANILPNSFHRLAHIQFVPAVLSLSIGILIVLIALTL
CCCEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGRIYCSTICPMGIWQDVIARISKSVGKKKKRYRYSPAKNMLRWTVLGVTVIASVCGFS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
VVLGLLDPYSAYGRIVVHIFKPVYMLGNNLLESVFSRFDNYTFYQVDTSVLSLSSLLIAI
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHH
LTFAAILILAWKHGRTWCNTICPVGTVLGFLSRYSLFKVRIDTAKCNGCGLCATKCKAAC
HHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHH
IHSKEHAIDYSRCVDCFDCLGACKQKALVYAPASKGQQRSSATEKQPVATESSSVAADSS
HCCHHHCCCHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
KRRFLLAGLATAGAAPTLLTKAQESIATMEGKKAYKKENPITPPGSVSQKRFQQHCTSCH
CCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LCVSKCPSHVLKPAFMEYGLGGVMQPTVSFEKGFCNFDCTVCSDVCPNGAIPPLTVEQKH
HHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCCHHH
LTQMGYVVFIEENCIVLTDGTSCGACSEHCPTQAIAMVPYKDGLTLPHVNTEICVGCGGC
HHHCCEEEEEECCEEEEECCCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCEEEEECCCC
EYVCPARPFRAVYIEGNPVQKEAKPFKESEEHKVEIDDFGF
CCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MLRKIRIGISVVLFALITFYFLDFANILPNSFHRLAHIQFVPAVLSLSIGILIVLIALTL
CCCEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGRIYCSTICPMGIWQDVIARISKSVGKKKKRYRYSPAKNMLRWTVLGVTVIASVCGFS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
VVLGLLDPYSAYGRIVVHIFKPVYMLGNNLLESVFSRFDNYTFYQVDTSVLSLSSLLIAI
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHH
LTFAAILILAWKHGRTWCNTICPVGTVLGFLSRYSLFKVRIDTAKCNGCGLCATKCKAAC
HHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHH
IHSKEHAIDYSRCVDCFDCLGACKQKALVYAPASKGQQRSSATEKQPVATESSSVAADSS
HCCHHHCCCHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
KRRFLLAGLATAGAAPTLLTKAQESIATMEGKKAYKKENPITPPGSVSQKRFQQHCTSCH
CCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LCVSKCPSHVLKPAFMEYGLGGVMQPTVSFEKGFCNFDCTVCSDVCPNGAIPPLTVEQKH
HHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCCHHH
LTQMGYVVFIEENCIVLTDGTSCGACSEHCPTQAIAMVPYKDGLTLPHVNTEICVGCGGC
HHHCCEEEEEECCEEEEECCCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCEEEEECCCC
EYVCPARPFRAVYIEGNPVQKEAKPFKESEEHKVEIDDFGF
CCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]