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The map label for this gene is glyA [H]

Identifier: 29346148

GI number: 29346148

Start: 916121

End: 917401

Strand: Reverse

Name: glyA [H]

Synonym: BT_0738

Alternate gene names: 29346148

Gene position: 917401-916121 (Counterclockwise)

Preceding gene: 29346149

Following gene: 29346146

Centisome position: 14.65

GC content: 47.15

Gene sequence:

>1281_bases
ATGAAAAGAGACGACTTAATTTTCGACATCATCGAAAAAGAGCACCAACGTCAGCTGAAAGGTATCGAGCTGATTGCATC
TGAAAACTTTGTAAGTGACCAGGTAATGCAGGCAATGGGTTCTTGCCTGACTAACAAGTACGCAGAAGGTTATCCGGGCA
AACGTTACTACGGAGGTTGTGAAGTAGTTGACCAAAGCGAACAAATCGCTATCGACCGCCTGAAAGAAATCTTCGGTGCC
GAATGGGCAAACGTACAACCGCATTCAGGAGCACAAGCCAATGCAGCCGTATTTCTGGCTGTTTTGAATCCAGGCGACAA
GTTTATGGGATTAAACCTCGCACACGGCGGACACCTTTCTCACGGTTCATTAGTAAACACTTCCGGCATTATCTACACTC
CTTGCGAATACAATCTGAATCAGGAAACCGGCCGTGTAGACTACGACCAGATGGAAGAAGTTGCTTTGCGCGAAAAGCCG
AAAATGATTATCGGCGGCGGCTCTGCTTATTCCCGCGAATGGGACTACAAACGTATGCGTGAAATCGCAGATAAAGTAGG
CGCTATCCTGATGATCGACATGGCTCACCCTGCGGGTCTGATCGCTGCCGGACTGTTGGAGAACCCGGTTAAATATGCAC
ACATCGTCACTTCGACTACACATAAAACACTTCGCGGACCTCGTGGTGGGGTCATCATGATGGGAAAAGATTTCCCTAAT
CCTTGGGGAAAGAAAACTCCGAAGGGTGAAATCAAAATGATGTCTCAATTACTGGATTCAGCTGTATTCCCAGGTGTACA
GGGCGGACCGTTGGAACACGTAATCGCTGCCAAAGCCGTAGCTTTCGGTGAAATCCTGCAACCGGAATACAAGGAATATG
CAAAACAGGTTCAGAAGAATGCTGCCATACTGGCACAGGCCTTGATAGACCGTGGTTTTACAATCGTTTCCGGTGGTACG
GACAATCACTCCATGCTGGTTGACCTGCGTAGCAAATACCCTGATCTGACAGGTAAGGTAGCAGAAAAAGCATTAGTTTC
TGCTGACATTACCGTGAATAAGAACATGGTTCCGTTTGACAGCCGTTCTGCTTTCCAAACTTCCGGTATCCGTCTGGGTA
CTCCTGCCATCACAACCCGTGGTGCGAAAGAAGACCTGATGATCGAAATTGCCGAAATGATCGAAACAGTTCTGTCTAAC
GTGGAAAATGAAGAGGTGATCGCACAAGTACGTGCACGTGTCAATGAGACAATGAAGAAATATCCTCTTTTTGCTGATTA
A

Upstream 100 bases:

>100_bases
ATTTTTCTTATAGTTCCTTCGTTCTTTATTTATTTTCACTAACTTTGTGCGCTTAAAATAGGTATCTCGAATTAAAAATT
TAATATTACTTATAAAAAGA

Downstream 100 bases:

>100_bases
GATTTCTTTCTTTGAGTAACCAAATGCCGGTAATGTGAATTAGCAGCATTTGTGTTCAACATTTCATAAAGAATTTCTTT
TGAATTTTATAAGCCTGCTT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKRDDLIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRLKEIFGA
EWANVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLSHGSLVNTSGIIYTPCEYNLNQETGRVDYDQMEEVALREKP
KMIIGGGSAYSREWDYKRMREIADKVGAILMIDMAHPAGLIAAGLLENPVKYAHIVTSTTHKTLRGPRGGVIMMGKDFPN
PWGKKTPKGEIKMMSQLLDSAVFPGVQGGPLEHVIAAKAVAFGEILQPEYKEYAKQVQKNAAILAQALIDRGFTIVSGGT
DNHSMLVDLRSKYPDLTGKVAEKALVSADITVNKNMVPFDSRSAFQTSGIRLGTPAITTRGAKEDLMIEIAEMIETVLSN
VENEEVIAQVRARVNETMKKYPLFAD

Sequences:

>Translated_426_residues
MKRDDLIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRLKEIFGA
EWANVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLSHGSLVNTSGIIYTPCEYNLNQETGRVDYDQMEEVALREKP
KMIIGGGSAYSREWDYKRMREIADKVGAILMIDMAHPAGLIAAGLLENPVKYAHIVTSTTHKTLRGPRGGVIMMGKDFPN
PWGKKTPKGEIKMMSQLLDSAVFPGVQGGPLEHVIAAKAVAFGEILQPEYKEYAKQVQKNAAILAQALIDRGFTIVSGGT
DNHSMLVDLRSKYPDLTGKVAEKALVSADITVNKNMVPFDSRSAFQTSGIRLGTPAITTRGAKEDLMIEIAEMIETVLSN
VENEEVIAQVRARVNETMKKYPLFAD
>Mature_426_residues
MKRDDLIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRLKEIFGA
EWANVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLSHGSLVNTSGIIYTPCEYNLNQETGRVDYDQMEEVALREKP
KMIIGGGSAYSREWDYKRMREIADKVGAILMIDMAHPAGLIAAGLLENPVKYAHIVTSTTHKTLRGPRGGVIMMGKDFPN
PWGKKTPKGEIKMMSQLLDSAVFPGVQGGPLEHVIAAKAVAFGEILQPEYKEYAKQVQKNAAILAQALIDRGFTIVSGGT
DNHSMLVDLRSKYPDLTGKVAEKALVSADITVNKNMVPFDSRSAFQTSGIRLGTPAITTRGAKEDLMIEIAEMIETVLSN
VENEEVIAQVRARVNETMKKYPLFAD

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=450, Percent_Identity=44, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI261862350, Length=450, Percent_Identity=44, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI261862348, Length=450, Percent_Identity=44, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI19923315, Length=450, Percent_Identity=44, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI261862346, Length=450, Percent_Identity=42.8888888888889, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI22547186, Length=426, Percent_Identity=46.0093896713615, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI22547189, Length=426, Percent_Identity=42.4882629107981, Blast_Score=295, Evalue=6e-80,
Organism=Escherichia coli, GI1788902, Length=422, Percent_Identity=56.1611374407583, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25144732, Length=434, Percent_Identity=43.778801843318, Blast_Score=350, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI25144729, Length=434, Percent_Identity=43.778801843318, Blast_Score=349, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6319739, Length=433, Percent_Identity=42.7251732101617, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=46.0199004975124, Blast_Score=323, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24640005, Length=456, Percent_Identity=43.859649122807, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=456, Percent_Identity=43.859649122807, Blast_Score=363, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46776; Mature: 46776

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDDLIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGC
CCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EVVDQSEQIAIDRLKEIFGAEWANVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLS
CEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
HGSLVNTSGIIYTPCEYNLNQETGRVDYDQMEEVALREKPKMIIGGGSAYSREWDYKRMR
CCCEEECCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
EIADKVGAILMIDMAHPAGLIAAGLLENPVKYAHIVTSTTHKTLRGPRGGVIMMGKDFPN
HHHHHCCEEEEEECCCCHHHHHHHHHHCCHHHEEEEECCHHHHHCCCCCCEEEECCCCCC
PWGKKTPKGEIKMMSQLLDSAVFPGVQGGPLEHVIAAKAVAFGEILQPEYKEYAKQVQKN
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AAILAQALIDRGFTIVSGGTDNHSMLVDLRSKYPDLTGKVAEKALVSADITVNKNMVPFD
HHHHHHHHHHCCCEEEECCCCCCEEEEEEHHHCCCHHHHHHHHHHHHCCEEECCCCCCCC
SRSAFQTSGIRLGTPAITTRGAKEDLMIEIAEMIETVLSNVENEEVIAQVRARVNETMKK
CCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YPLFAD
CCCCCC
>Mature Secondary Structure
MKRDDLIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGC
CCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
EVVDQSEQIAIDRLKEIFGAEWANVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLS
CEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
HGSLVNTSGIIYTPCEYNLNQETGRVDYDQMEEVALREKPKMIIGGGSAYSREWDYKRMR
CCCEEECCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
EIADKVGAILMIDMAHPAGLIAAGLLENPVKYAHIVTSTTHKTLRGPRGGVIMMGKDFPN
HHHHHCCEEEEEECCCCHHHHHHHHHHCCHHHEEEEECCHHHHHCCCCCCEEEECCCCCC
PWGKKTPKGEIKMMSQLLDSAVFPGVQGGPLEHVIAAKAVAFGEILQPEYKEYAKQVQKN
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AAILAQALIDRGFTIVSGGTDNHSMLVDLRSKYPDLTGKVAEKALVSADITVNKNMVPFD
HHHHHHHHHHCCCEEEECCCCCCEEEEEEHHHCCCHHHHHHHHHHHHCCEEECCCCCCCC
SRSAFQTSGIRLGTPAITTRGAKEDLMIEIAEMIETVLSNVENEEVIAQVRARVNETMKK
CCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YPLFAD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA