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The map label for this gene is ispE

Identifier: 29346034

GI number: 29346034

Start: 768864

End: 769688

Strand: Reverse

Name: ispE

Synonym: BT_0624

Alternate gene names: 29346034

Gene position: 769688-768864 (Counterclockwise)

Preceding gene: 29346047

Following gene: 29346004

Centisome position: 12.29

GC content: 46.55

Gene sequence:

>825_bases
ATGATCACTTTCCCAAACGCAAAAATAAACCTGGGACTCTCTATTACCGAGAAGCGTCCGGACGGATATCACAACCTCGA
AACCGTATTCTACCCCGTCGCTCTCGAAGATGCGCTGGAAATCCGGACCAGCCCCGAAGCGGACAAAAAGTTCTCCCTGC
ATCAGTACGGAATGGAGATATCCGGTAATCCGGAAGACAATTTAGTGGTGAAAGCATATCTGCTATTAGATAAAGAGTTT
CACCTCTGTCCTATCGAGATCCATCTGTACAAACACATTCCTTCCGGTGCCGGACTGGGAGGAGGCTCATCAGACGCGGC
TTTCATGCTGAAATTACTCAACGAACACTTTCAGCTGAATTTATCCGAAGATCAGCTGGAAATATATGCCGCAACTTTGG
GAGCAGACTGCGCTTTCTTTATCAGAAACGCCCCGACCTTTGCCGAAGGAGTCGGTAACATATTCTCCCCCATTCCACTA
TCCTTAAAAGGATATCAGATTCTGATCATCAAACCGGATGTATTCGTATCAACGCGGGAAGCTTTCGCCAATATTCACCC
TCATCATCCCGAATACTCGATCAAAGAAGCGATCAAACGCCCGGTAAATGAATGGAAAGAGATATTAATCAATGATTTTG
AAGACAGCGTATTCCCCCAGCATCCGGTGATCGGAGAAATTAAAGCCGAGCTATACAGGCAAGGGGCTGTTTATGCCTCT
ATGAGCGGTTCCGGTTCTTCCGTATATGGATTGTTCGAGCCGGAAGGGACTTTACCTGAAACTGATTGGGGAACTAACGT
ATTCTGTTTTAAAGGCAGGCTGTAA

Upstream 100 bases:

>100_bases
GAAAAGATAAGGAAAAATGAAGATTTTAGTAACTTTGTCACCAATAGCATAAATTCGCAACCCATAATTTAAAATTCAAA
AATAATAATTCAGAACGAAT

Downstream 100 bases:

>100_bases
AAGAGTAACAAAAAACGGTACCACGACTCCCGTCGTAGTACCGTCACAACACAAACACAAAATAAAACACGACAAAACTA
CTATGCAATCATACCTGTCT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MITFPNAKINLGLSITEKRPDGYHNLETVFYPVALEDALEIRTSPEADKKFSLHQYGMEISGNPEDNLVVKAYLLLDKEF
HLCPIEIHLYKHIPSGAGLGGGSSDAAFMLKLLNEHFQLNLSEDQLEIYAATLGADCAFFIRNAPTFAEGVGNIFSPIPL
SLKGYQILIIKPDVFVSTREAFANIHPHHPEYSIKEAIKRPVNEWKEILINDFEDSVFPQHPVIGEIKAELYRQGAVYAS
MSGSGSSVYGLFEPEGTLPETDWGTNVFCFKGRL

Sequences:

>Translated_274_residues
MITFPNAKINLGLSITEKRPDGYHNLETVFYPVALEDALEIRTSPEADKKFSLHQYGMEISGNPEDNLVVKAYLLLDKEF
HLCPIEIHLYKHIPSGAGLGGGSSDAAFMLKLLNEHFQLNLSEDQLEIYAATLGADCAFFIRNAPTFAEGVGNIFSPIPL
SLKGYQILIIKPDVFVSTREAFANIHPHHPEYSIKEAIKRPVNEWKEILINDFEDSVFPQHPVIGEIKAELYRQGAVYAS
MSGSGSSVYGLFEPEGTLPETDWGTNVFCFKGRL
>Mature_274_residues
MITFPNAKINLGLSITEKRPDGYHNLETVFYPVALEDALEIRTSPEADKKFSLHQYGMEISGNPEDNLVVKAYLLLDKEF
HLCPIEIHLYKHIPSGAGLGGGSSDAAFMLKLLNEHFQLNLSEDQLEIYAATLGADCAFFIRNAPTFAEGVGNIFSPIPL
SLKGYQILIIKPDVFVSTREAFANIHPHHPEYSIKEAIKRPVNEWKEILINDFEDSVFPQHPVIGEIKAELYRQGAVYAS
MSGSGSSVYGLFEPEGTLPETDWGTNVFCFKGRL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=256, Percent_Identity=33.984375, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_BACTN (Q8AA41)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809537.1
- ProteinModelPortal:   Q8AA41
- SMR:   Q8AA41
- GeneID:   1074354
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0624
- NMPDR:   fig|226186.1.peg.624
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- PhylomeDB:   Q8AA41
- ProtClustDB:   PRK14609
- BioCyc:   BTHE226186:BT_0624-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30534; Mature: 30534

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITFPNAKINLGLSITEKRPDGYHNLETVFYPVALEDALEIRTSPEADKKFSLHQYGMEI
CCCCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCCHHHCCCCCCCCCCEEHHHCCEEE
SGNPEDNLVVKAYLLLDKEFHLCPIEIHLYKHIPSGAGLGGGSSDAAFMLKLLNEHFQLN
CCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHEEC
LSEDQLEIYAATLGADCAFFIRNAPTFAEGVGNIFSPIPLSLKGYQILIIKPDVFVSTRE
CCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCEECCEEEEEECCCCEEECHH
AFANIHPHHPEYSIKEAIKRPVNEWKEILINDFEDSVFPQHPVIGEIKAELYRQGAVYAS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEE
MSGSGSSVYGLFEPEGTLPETDWGTNVFCFKGRL
ECCCCCEEEEEECCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MITFPNAKINLGLSITEKRPDGYHNLETVFYPVALEDALEIRTSPEADKKFSLHQYGMEI
CCCCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCCHHHCCCCCCCCCCEEHHHCCEEE
SGNPEDNLVVKAYLLLDKEFHLCPIEIHLYKHIPSGAGLGGGSSDAAFMLKLLNEHFQLN
CCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHEEC
LSEDQLEIYAATLGADCAFFIRNAPTFAEGVGNIFSPIPLSLKGYQILIIKPDVFVSTRE
CCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCEECCEEEEEECCCCEEECHH
AFANIHPHHPEYSIKEAIKRPVNEWKEILINDFEDSVFPQHPVIGEIKAELYRQGAVYAS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEE
MSGSGSSVYGLFEPEGTLPETDWGTNVFCFKGRL
ECCCCCEEEEEECCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928